Melemo ea rona ke litheko tse fokotsehileng, basebetsi ba thekiso ea lihlahisoa tse matla, QC e khethehileng, lifeme tse tiileng, lits'ebeletso tsa boleng bo holimo bakeng sa Low MOQ bakeng sa China Real-Time Quantitative PCR Machine Thermal Cycler PCR System PCR Tube Clinical Analytical Instruments, Re tlotla mosuoe oa rona oa mantlha oa Botšepehi khoebong, tse tlang pele ho fana ka litšebeletso tsa boleng bo holimo le barekisi ba rona ba tla fana ka litšebeletso tsa boleng bo holimo.
Melemo ea rona ke litheko tse fokotsehileng, basebetsi ba thekiso ea lihlahisoa tse matla, QC e khethehileng, lifeme tse tiileng, lits'ebeletso tsa boleng bo holimo bakeng saTlhahlobo e Ikemetseng ea Nucleic Acid, Lisebelisoa tsa ho Khetholla tsa China Rt PCR, Ka lebaka la boinehelo ba rona, thepa ea rona e tsebahala lefatšeng ka bophara mme palo ea rona ea thepa e romelloang e ntse e hola selemo le selemo.Re tla tsoela pele ho ikitlaelletsa ho sebetsa hantle ka ho fana ka lintho tsa boleng bo holimo tse tla feta tebello ea bareki ba rona.

Litlhaloso tsa Kits

50 Preps, 200 Preps

Setsi sena se sebelisa "spin column le formula" e entsoeng ke k'hamphani ea rona, e ka ntšang RNA e hloekileng le ea boleng bo phahameng ka ho fetisisa ho tsoa ho mefuta e sa tšoaneng ea liphoofolo tse nang le bokhoni bo phahameng.E fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo le ho bapatsa DNA ea genomic ho tloha ho supernatant le lysate ea lisele, e bonolo le e bolokang nako;Kholomo ea RNA-feela e ka tlama RNA hantle 'me e ka sebetsoa ka nako e le 'ngoe ka foromo e ikhethang Mehlala e mengata.

Tsamaiso eohle ke RNase-Free, e le hore RNA e nkiloeng e se ke ea senyeha;Buffer RW1, Buffer RW2 buffer system ea ho hlatsoa, ​​e le hore RNA e fumanoeng e se na protheine, DNA, ion, le tšilafalo ea motsoako oa organic.

Likarolo tsa Kit:

Likarolo le melemo

■ Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA;tsamaiso eohle ke RNase-Free
■ Tlosa ka katleho DNA-sebelisa DNA-Cleaning Column
■ Tlosa DNA ntle le ho eketsa DNase
■ Ts'ebetso e bonolo-tsohle e phethoa ka mocheso oa kamore
■ Ts'ebetso e potlakileng e ka phethoa ka mor'a metsotso e 30
■ Sireletsehile-ha ho na reagent ea tlhaho e hlokahalang
■ Bohloeki bo phahameng -OD260/280≈1.8-2.1

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa mefuteng e fapaneng ea lithane tsa liphoofolo tse foreshe kapa tse hatselitsoeng kapa lisele tse holileng.

Lihlahisoa tsa lihlahisoa

■ Lisebelisoa tse tlase: motsoako oa pele oa cDNA, RT-PCR, cloning ea molek'hule, Northern Blot, joalo-joalo.
■ Mehlala: lisele tsa liphoofolo, lisele tse hōlileng tsebong
■ Tekanyetso: Li-tissues 10-20mg, Lisele (2-5)×10⁶
■ Boholo bo tlamang DNA ea kholomo ea tlhoekiso: 80 μg
■ Molumo oa elution: 50-200 μl

Phallo ea mosebetsi

Setšoantšo

Animal Total RNA Isolation Kit tšoaroa 20mg
Fresh mouse disampole, nka 5% hloekisitsoeng kakaretso RNA 1% agar

Glycogel electrophoresis
1: Lesapo la 2: Liphio
3: Sebete 4: Pelo

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 24 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele.Buffer RL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli ea 1 ka mor'a ho eketsa β- mercaptoethanol (ho ikhethela).

Melemo ea rona ke litheko tse fokotsehileng, basebetsi ba thekiso ea lihlahisoa tse matla, QC e khethehileng, lifeme tse tiileng, lits'ebeletso tsa boleng bo holimo bakeng sa Low MOQ bakeng sa China Real-Time Quantitative PCR Machine Thermal Cycler PCR System PCR Tube Clinical Analytical Instruments, Re tlotla mosuoe oa rona oa mantlha oa Botšepehi khoebong, tse tlang pele ho fana ka litšebeletso tsa boleng bo holimo le barekisi ba rona ba tla fana ka litšebeletso tsa boleng bo holimo.
MOQ e tlase bakeng saLisebelisoa tsa ho Khetholla tsa China Rt PCR, Tlhahlobo e Ikemetseng ea Nucleic Acid, Ka lebaka la boinehelo ba rona, thepa ea rona e tsebahala lefatšeng ka bophara mme palo ea rona ea thepa e romelloang e ntse e hola selemo le selemo.Re tla tsoela pele ho ikitlaelletsa ho sebetsa hantle ka ho fana ka lintho tsa boleng bo holimo tse tla feta tebello ea bareki ba rona.