E nang le maikutlo a matle le a tsoelo-pele ho khahleng ea bareki, khoebo ea rona e lula e ntlafatsa thepa ea rona ho khotsofatsa litakatso tsa bareki mme e shebane le polokeho, ts'epahalo, litlhoko tsa tikoloho, le boqapi ba Moetsi oa China Magnetic Beads Nucleic Acid PCR Extraction Kit e nang le CE bakeng sa Mochini oa Poct PCR, ka 'nete re hola ka ts'epo ea rona.
E nang le maikutlo a matle le a tsoelo-pele ho khahleng ea bareki, khoebo ea rona e lula e ntlafatsa thepa ea rona ho khotsofatsa litakatso tsa bareki mme e shebane le polokeho, ts'epahalo, litlhoko tsa tikoloho le boqapiChina DNA Extraction Kit, Nucleic Acid Extract Kit, Re khomarela moreki oa 1st, boleng bo holimo ba 1, ntlafatso e tsoelang pele, melemo e kopanetsoeng le melao-motheo ea win-win.Ha re sebelisana 'moho le moreki, re isetsa bareki ba nang le litšebeletso tsa boleng bo holimo.Re thehile likamano tse ntle tsa khoebo re sebelisa moreki oa Zimbabwe ka har'a khoebo, re iketselitse lebitso le botumo ba rona.Ka nako e ts'oanang, amohela ka pelo eohle litebello tse ncha le tsa khale k'hamphaning ea rona ea ho ea le ho buisana le likhoebo tse nyane.

Litlhaloso

Setsi sena se sebelisa sistimi e ikhethileng ea lysis buffer e ka lokollang RNA kapele ho lisampole tsa lisele tse hlahisitsoeng bakeng sa karabelo ea RT-qPCR, ka hona e felisa ts'ebetso ea tlhoekiso ea RNA e jang nako le e boima.Setšoantšo sa RNA se ka fumanoa ka metsotso e 7 feela.The 5×Direct RT Mix le 2×Direct qPCR Mix-SYBR reagents e fanoeng ke kit e ka fumana kapele le ka nepo liphetho tsa nako ea nnete tsa PCR.

5 × Direct RT Mix le 2 × Direct qPCR Mix-SYBR li na le mamello e matla ea inhibitor, 'me lysate ea lisampole e ka sebelisoa e le template ea RT-qPCR ka ho toba.Setsi sena se na le RNA e phahameng ea kamano e phahameng ea Foregene reverse transcriptase, le Hot D-Taq DNA polymerase, dNTPs, MgCl.₂, buffer ea karabelo, PCR optimizer le stabilizer.

Litlhaloso

200×20μl Rxns, 1000×20μl Rxns

Likarolo tsa Kit

Likarolo le melemo

■ E bonolo ebile e sebetsa : ka theknoloji ea Cell Direct RT, lisampole tsa RNA li ka fumanoa ka metsotso e 7 feela.

■ Tlhokahalo ea sampole e nyane, e ka tlase ho lisele tse 10 li ka lekoa.

■ Phallo e phahameng: e khona ho lemoha ka potlako RNA liseleng tse entsoeng ka lipoleiti tse 384, 96, 24, 12, 6.

■ DNA Eraser e ka tlosa ka potlako liphatsa tsa lefutso tse lokollotsoeng, ea fokotsa haholo tšusumetso liphethong tse latelang tsa liteko.

■ Optimized RT le qPCR system e etsa hore mehato e 'meli ea RT-PCR reverse transcript e sebetse hantle le PCR e be e hlakileng haholoanyane, 'me e hanane le RT-qPCR reaction inhibitors.

Sesebelisoa sa Kit

Sekhahla sa ts'ebeliso: lisele tse tsoetseng pele.

- RNA e lokollotsoe ka mohlala oa lysis: e sebetsa feela ho template ea RT-qPCR ea kit ena.

- Thepa e ka sebelisoa bakeng sa merero e latelang: tlhahlobo ea polelo ea liphatsa tsa lefutso, netefatso ea siRNA-mediated gene silence effect, tlhahlobo ea lithethefatsi, joalo-joalo.

Setšoantšo

Storage le Shelf bophelo

Karolo ea I ea kit ena e lokela ho bolokoa ho 4 ℃;Karolo ea II e lokela ho bolokoa ho -20 ℃.

Foregene Protease Plus II e lokela ho bolokoa mochesong oa 4 ℃, e se ke ea hoama ho -20℃.

Reagent 2 × Direct qPCR Mix-SYBR e lokela ho bolokoa ho -20 ℃ lefifing;haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4 ℃ bakeng sa polokelo ea nako e khutšoanyane (sebelisa ka hare ho matsatsi a 10) . E nang le boikutlo bo botle le bo tsoelang pele ho khahloa ke bareki, khoebo ea rona e lula e ntlafatsa ntho ea rona e babatsehang ho khotsofatsa litakatso tsa bareki 'me e shebane le ts'ireletso, botšepehi, litlhoko tsa tikoloho, le popontshwa ea Moetsi oa Acculation bakeng sa Mochini oa Mochine o motlakase oa China CER, China Nuclear Producer. Ke tšepa ka tieo hore re ntse re hola hammoho le litebello tsa rona tikolohong eohle.
Moetsi bakeng saChina DNA Extraction Kit, Nucleic Acid Extract Kit, Re khomarela moreki oa 1st, boleng bo holimo ba 1, ntlafatso e tsoelang pele, melemo e kopanetsoeng le melao-motheo ea win-win.Ha re sebelisana 'moho le moreki, re isetsa bareki ba nang le litšebeletso tsa boleng bo holimo.Re thehile likamano tse ntle tsa khoebo re sebelisa moreki oa Zimbabwe ka har'a khoebo, re iketselitse lebitso le botumo ba rona.Ka nako e ts'oanang, amohela ka pelo eohle litebello tse ncha le tsa khale k'hamphaning ea rona ea ho ea le ho buisana le likhoebo tse nyane.