Phaliso e Ncha bakeng sa China Cell Total Rna Isolation Kit Rnase-Free CE
Ka kakaretso re lumela hore sebopeho sa motho se etsa qeto ea boleng ba lihlahisoa tsa boleng bo holimo, lintlha li etsa qeto ea hore lihlahisoa tsa boleng bo holimo, hammoho le moea oa sehlopha sa REALISTIC, EFFICIENT AND INNOVATIVE bakeng sa New Delivery bakeng saChina Cell Total Rna Isolation KitRnase-Free CE, Theko e rekisoang e nang le boleng bo holimo le lits'ebeletso tse khotsofatsang li etsa hore re fumane bareki ba bangata haholo. re lakatsa ho sebetsa le uena le ho batla ntlafatso e tšoanang.
Ka kakaretso re lumela hore sebopeho sa motho se etsa qeto ea boleng ba lihlahisoa tsa boleng bo holimo, lintlha li etsa qeto ea hore lihlahisoa tsa boleng bo holimo, hammoho le moea oa sehlopha sa REALISTIC, SEBELISA LE HO HLAHALASele, China Cell Total Rna Isolation Kit, Khamphani ea rona e se e fetile maemo a ISO 'me re hlompha ka botlalo litokelo le litokelo tsa bareki ba rona.Haeba moreki a fana ka meralo ea hae, Re tla netefatsa hore mohlomong ke bona feela ba ka bang le thepa eo.Re tšepa hore ka lihlahisoa tsa rona tse ntle li ka tlisetsa bareki ba rona mahlohonolo a maholo.
Tlhaloso
50 Preps, 200 Preps
Setsi sena se sebelisa "spin column le formula" e ntlafalitsoeng ke Foregene, e ka ntšang RNA ea boleng bo holimo le ea boleng bo holimo liseleng tse entsoeng ka lipoleiti tse 96, 24, 12 le 6.
Thepa e fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo supernatant le cell lysate, ho tlama le ho tlosa DNA ea genomic.Opereishene e bonolo ebile e boloka nako.
Kholomo ea RNA-feela e ka tlama RNA hantle ka foromo e ikhethang.Palo e kholo ea disampole e ka sebetswa ka nako e le nngwe.
Likarolo tsa Kit
| Sebopeho sa Kit | RE-03111 | RE-03114 |
| 50 T | 200 T | |
| Buffer cRL1* | 25 ml | 100 ml |
| Buffer cRL2 | 15 ml | 60 ml |
| Buffer RW1* | 25 ml | 100 ml |
| Buffer RW2 | 24 ml | 96 ml |
| RNase-Free ddH2O | 10 ml | 40 ml |
| RNA-Feela Kholomo | 50 | 200 |
| DNA-Ho hloekisa Column | 50 | 200 |
| Taelo | 1 | 1 |
*Ka kopo, roala liatlana 'me u nke mehato ea ts'ireletso nakong ea ts'ebetso kaha Buffer cRL1 le Buffer RW1 li na le matsoai a chaotropic a halefisang.
Likarolo le melemo
■ Tshebetso eohle e sebetsa mocheso oa kamore (15-25 ℃), ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Thepa eohle ha e na RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa ka metsotso e 11.
■ Polokeho: Ha ho hlokahale reagent ea tlhaho.
■ Boleng bo phahameng: RNA e hloekisitsoeng e hloekile haholo, ha e na protheine le litšila tse ling, ’me e ka kopana le liteko tse sa tšoaneng tse latelang.
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa liseleng tse hlahisitsoeng ka har'a lipoleiti tse 96, 24, 12 le 6 tsa seliba.
Phallo ea mosebetsi
Setšoantšo
Setšoantšo sa betri ea gel ea agarose eaSeleKakaretso ea RNA Isolation Kit e tšoaroa ka holimo ho palo e fapaneng ea lisele, 20μl bophahamo ba modumo, nka 2μl hloekisitsoeng kakaretso RNA 1%.
Polokelo le shelf bophelo
Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele (likhoeli tse 24).
Buffer cRL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho eketsa 2-hydroxy-1-ethanethiol(optional).Ka kakaretso re lumela hore sebopeho sa motho se etsa qeto ea boleng ba lihlahisoa, lintlha li etsa qeto ea hore lihlahisoa' tsa boleng bo phahameng, hammoho le moea oa sehlopha sa REALISTIC, EFFICIENT AND INNOVATIVE bakeng sa New Delivery for China.SeleTotal Rna Isolation Kit Rnase-Free ISO FDA CE, Theko ea ho rekisa e mabifi e nang le boleng bo holimo le lits'ebeletso tse khotsofatsang li etsa hore re fumane chelete e ngata ho feta bareki.re lakatsa ho sebetsa le uena le ho batla ntlafatso e tloaelehileng.
Thekiso e Ncha ea China Cell Total Rna Isolation Kit, Cell, Khamphani ea rona e se e fetile maemo a ISO 'me re hlompha litokelo tsa molao tsa bareki ba rona ka botlalo.Haeba moreki a fana ka meralo ea hae, Re tla netefatsa hore mohlomong ke bona feela ba ka bang le thepa eo.Re tšepa hore ka lihlahisoa tsa rona tse ntle li ka tlisetsa bareki ba rona mahlohonolo a maholo.
