Qalong ea koluoa ​​​​eo, ka lebaka la nts'etsopele e potlakileng, tlhahlobo e potlakileng ea bakuli ba belaelloang ke senotlolo sa ho thibela COVID-19.Li-reagents tse ling tse amohetsoeng tsa nucleic acid li na le nako e khuts'oane ea nts'etsopele, 'me ho na le mathata a kang netefatso ea ts'ebetso e potlakileng, ts'ebetso e sa lekaneng ea reagent, le phapang e kholo lipakeng tsa lihlopha;Mathata a lilaboratori tse fapaneng tsa kliniki likarolong tse fapaneng tsa ts'ebetso ea ho lemoha ha nucleic acid le 'ona a ka ama ho nepahala ha sephetho sa ho sibolla ha nucleic acid.Sengoliloeng sena se tla shebana le likhokahano tsa bohlokoa le lintlha tsa tlhahlobo ea morao-rao ea SARS-CoV-2 nucleic acid, le ho sekaseka mathata a tlhahlobo e fosahetseng le e ntle ea tlhahlobo ea laboratori ea nucleic acid le ho se lumellane ha kliniki.

Melao-motheo ea ho lemoha ha SARS-CoV-2 nucleic acid

SARS-CoV-2 ke vaerase ea RNA e nang le tatellano ea genome e ka bang 29 kb, e nang le liphatsa tsa lefutso tse 10, tse ka kenyelletsang liprotheine tse 10 ka nepo.Livaerase li entsoe ka RNA le protheine, 'me karolo e ka ntle ke lesela le ka ntle le entsoeng ka lipids le glycoprotein.Ka hare, protheine capsid e phuthela RNA ho eona, kahoo e sireletsa RNA (P1) e senyehang habonolo.

P1 Sebopeho sa SARS-COV-2

Livaerase li hlasela lisele ka li-receptor tse itseng ho baka tšoaetso, 'me li sebelisa lisele tse amohelang batho ho ikatisa.

Molao-motheo oa ho lemoha ha varal nucleic acid ke ho pepesa RNA ea kokoana-hloko ka cell lysate, ebe o sebelisa nako ea sebele ea fluorescent reverse transcription-polymerase chain reaction (RT-PCR) bakeng sa ho lemoha.

Senotlolo sa molao-motheo oa ho sibolla ke ho sebelisa li-primers le li-probes ho fihlela "tekanyo e reriloeng" ea tatellano ea nucleic acid, ke hore, ho fumana tatellano ea nucleic acid ea SARS-CoV-2 e fapaneng le livaerase tse ling ka metheo e ka bang 30,000 (ho tšoana ha nucleic acid ho livaerase tse ling) "Low" sebaka), li-primers le probes.

Li-primers le probes li tsamaellana haholo le sebaka se ikhethileng sa SARS-CoV-2 nucleic acid, ke hore, boits'oaro bo matla haholo.Hang ha sephetho sa nako ea nnete sa fluorescent RT-PCR sa sampole e tla hlahlojoa se le ntle, se paka hore SARS-CoV-2 e teng sampoleng.Sheba P2.

Mehato ea P2 ea boikemisetso ba SARS-CoV-2 nucleic acid (ka nako ea nnete ea fluorescent RT-PCR)

Maemo le litlhoko tsa laboratori bakeng sa ho lemoha ha SARS-CoV-2 nucleic acid

Lilaboratori tsa tlhahlobo ea Nucleic acid ke tsona tse loketseng maemo a khatello e mpe, 'me li lokela ho ela hloko khatello ea maikutlo, ho boloka moea o phalla le ho felisa li-aerosol.Basebeletsi ba tlhahlobo ea Nucleic acid ba tlameha ho ba le mangolo a thuto a lumellanang, ba fumane koetliso e nepahetseng ea polymerase chain reaction mme ba fetise tlhahlobo.Laboratori e lokela ho laoloa ka thata, e beheloe sebakeng, 'me basebetsi ba sa amehang ba thibetsoe ka thata ho kena.Sebaka se hlwekileng se lokela ho kenngwa moya le ho hlatswa dikokwana-hloko sebakeng.Lintho tse amehang li behoa libakeng, tse hloekileng le tse litšila lia aroloa, li nkeloa sebaka ka nako, 'me li silafalitsoe sebakeng seo.Ho bolaea likokoana-hloko ka tloaelo: Sebolaea-mafu se nang le klorine ke tharollo ea mantlha libakeng tse kholoanyane, 'me joala ba 75% bo ka sebelisoa libakeng tse nyane.Mokhoa o motle oa ho sebetsana le li-aerosol ke ho bula lifensetere bakeng sa moea, 'me ho bolaea likokoana-hloko moeeng ho ka boela ha etsoa ka mahlaseli a ultraviolet, filtration le moea o bolaeang likokoana-hloko.

Lihokelo tsa bohlokoa le liparamente tsa SARS-CoV-2 nucleic acid determination (real-time fluorescent RT-PCR)

Le hoja lilaboratori ka kakaretso li ela hloko "ho lemoha" ha nucleic acid, ha e le hantle, "ho ntšoa" ha "nucleic acid" hape ke e 'ngoe ea mehato ea bohlokoa ea ho lemoha ka katleho, e amanang haufi-ufi le ho bokella le ho boloka lisampole tsa kokoana-hloko.

Hona joale, lisebelisoa tsa phefumoloho tse sebelisoang ka ho fetisisa, tse kang nasopharyngeal swabs, li sebelisa mokhoa oa bobeli, e leng tharollo e sa sebetseng (ho boloka) e lokiselitsoeng ho latela nucleic acid extract le lysis solution.Ka lehlakoreng le leng, tharollo ena ea ho boloka kokoana-hloko e ka senya protheine ea kokoana-hloko, ea lahleheloa ke ts'ebetso ea eona 'me ha e sa hlola e tšoaetsanoa,' me ea ntlafatsa tšireletseho ea sethala sa lipalangoang le ho lemoha;ka lehlakoreng le leng, e ka pepesa kokoana-hloko ka ho toba ho lokolla nucleic acid, ea felisa enzyme e senyang ea nucleic, le ho thibela kokoana-hloko.RNA e fokotsehile.

Tharollo ea sampole ea kokoana-hloko e lokiselitsoeng motheong oa tharollo ea nucleic acid extract lysis.Likarolo tse ka sehloohong ke matsoai a leka-lekaneng, ethylenediaminetetraacetic acid chelating agent, letsoai la guanidine (guanidine isothiocyanate, guanidine hydrochloride, joalo-joalo), anionic surfactant (dodecane) sodium sulfate), cationic surfactant (tetradecyl methyloxalate, hydroxyline, hydrochloride, hydrochloride, hydrochloride), proteinase K le likarolo tse ling tse 'maloa kapa ho feta.Hona joale, ho na le mefuta e mengata ea lisebelisoa tsa ho ntša nucleic acid, 'me ho sebelisoa li-reagents tse fapaneng tsa nucleic acid le ho hloekisa.Esita le haeba ho sebelisoa nucleic acid e le 'ngoe le reagent ea ho hloekisa, mekhoa ea ho ntša sehlahisoa ka seng e fapane.

Hajoale, lihlahisoa tsa ho lemoha li-nucleic acid tse amohetsoeng ke Tsamaiso ea Naha ea Lihlahisoa tsa Bongaka li khethoa ho ipapisitse le liphatsa tsa lefutso tsa ORF1ab, E le N ho genome ea SARS-CoV-2.Melao-motheo ea ho lemoha lihlahisoa tse fapaneng ha e le hantle e tšoana, empa li-primers tsa tsona le meralo ea lipatlisiso li fapane.Ho na le likarolo tsa sepheo se le seng (ORF1ab), likarolo tsa sepheo se le seng (ORF1ab, N kapa E), le likarolo tse tharo (ORF1ab, N le E).Phapang pakeng tsa ho lemoha le ho hlalosa, nucleic acid extraction le real-time fluorescent RT-PCR reaction system e lokela ho bua ka litaelo tse loketseng tsa kit, 'me ho kgothaletswa hore basebelisi ba latele ka tieo mokhoa oa ho hlalosa o boletsoeng litaelong tsa kit bakeng sa tlhaloso.Libaka tse tloaelehileng, li-primers le tatellano ea lipatlisiso tse matlafalitsoeng ke RT-PCR ea nako ea nnete ea fluorescent li bonts'oa ho P3.

P3 Sebaka sa SARS-CoV-2 amplicon target ho genome le tatellano ea li-primers le probes.

Tlhaloso ea liphetho tsa qeto ea SARS-CoV-2 nucleic acid (Real-TIme fluorescent RT-PCR)

"Leano la Thibelo le Taolo ea Pneumonia bakeng sa Tšoaetso ea SARS-CoV-2 (Khatiso ea Bobeli)" ka lekhetlo la pele e hlakisitse mekhoa ea ho ahlola sephetho sa phatsa ea lefutso e le 'ngoe:

1. Ha ho Ct kapa Ct≥40 e mpe;

2. Ct <37 e ntle;

3. Theko ea Ct ea 37-40 ke sebaka sa grey-scale.Ho kgothaletswa ho pheta teko.Haeba sephetho sa ho pheta-pheta Ct <40 le curve ea amplification e na le litlhoro tse hlakileng, sampole e nkoa e le ntle, ho seng joalo e fosahetse. ”

Khatiso ea boraro ea tataiso le khatiso ea bone ea tataiso e ile ea tsoela pele ka mekhoa e ka holimo.Leha ho le joalo, ka lebaka la lipheo tse fapaneng tse sebelisoang ka lik'hamphani tsa khoebo, khatiso ea 3 e boletsoeng ka holimo ea tataiso ha ea ka ea fana ka litekanyetso tsa ho khetholla ho kopana ha lipakane, ho hatisa Litaelo tse fanoeng ke moetsi li tla ba teng.Ho qala ho tloha khatisong ea bohlano ea litataiso, lipakane tse peli li hlalositsoe, haholo-holo litekanyetso tsa kahlolo bakeng sa sepheo se le seng seo ho leng thata ho se ahlola.Ka mantsoe a mang, haeba laboratori e batla ho netefatsa hore nyeoe e nepahetse bakeng sa ho sibolloa ha nucleic acid ea SARS-CoV-2, tse latelang li hloka ho finyelloa 1 ho maemo a 2:

(1) Liphehelo tse peli tsa SARS-CoV-2 (ORF1ab, N) sampoleng e tšoanang li lekoa li le ntle ke RT-PCR ea nako ea nnete ea fluorescent.Haeba pakane e le 'ngoe e le ntle, ho hlokahala lisampole hape le ho hlahlojoa hape.Haeba sephetho sa liteko ke Haeba sepheo se le seng se ntse se le ntle, se nkoa e le ntle.

(2) Mehlala e 'meli ea nako ea sebele ea fluorescent RT-PCR e bontšitse sepheo se le seng se nepahetseng ka nako e le' ngoe kapa mehlala e 'meli ea mofuta o le mong e bontšitse sephetho se le seng se nepahetseng sa tlhahlobo, se ka nkoang se le ntle.Leha ho le joalo, litataiso li boetse li totobatsa hore liphetho tse mpe tsa tlhahlobo ea nucleic acid li ke ke tsa qhelela ka thoko tšoaetso ea SARS-CoV-2.Lintlha tse ka bakang mefokolo ea bohata li hloka ho qheleloa ka thoko, ho kenyelletsa boleng bo bobe ba sampole (sampole ea phefumoloho e tsoang ho oropharynx le likarolo tse ling), pokello ea sampole e sa le pele haholo kapa e se e le morao haholo, lisampole ha lia ka tsa bolokoa, tsa tsamaisoa le ho sebetsoa ka nepo, 'me theknoloji ka boeona e bile le mathata (ho fapana ha kokoana-hloko, PCR inhibition), joalo-joalo.

Lisosa tsa li-negative tsa bohata ho fumanoeng SARS-CoV-2

Mohopolo oa "mashano a fosahetseng" tlhahlobong ea nucleic acid e ntseng e ameha hajoale, hangata e bua ka "lintho tse fosahetseng" tseo ho tsona liphetho tsa tlhahlobo ea nucleic acid li sa lumellaneng le lipontšo tsa kliniki, ke hore, matšoao a kliniki le liphetho tsa litšoantšo li belaelloa haholo ke COVID-19, empa liteko tsa nucleic acid li lula li le "mpe" hangata.Setsi sa Clinical Laboratory Center sa Khomishene ea Naha ea Bophelo bo Botle se hlalositse tlhahlobo ea "SARS-CoV-2" ea bohata.

(1) Ho na le tekanyo e itseng ea kokoana-hloko liseleng tsa motho ea tšoaelitsoeng.Lintlha tse teng li bontša hore ka mor'a hore 'mele o tšoaelitsoe ke kokoana-hloko, kokoana-hloko e kena' metso ka nko le molomo, ebe e ea trachea le bronchi, ebe e fihla ho alveoli.Motho ea tšoaelitsoeng o tla ba le nako ea ho qhotsa, matšoao a bobebe, ebe joale ts'ebetso ea matšoao a matla, le mekhahlelo e fapaneng ea lefu lena.'Me palo ea kokoana-hloko e teng likarolong tse fapaneng tsa' mele e fapane.

Ho ea ka bongata ba kokoana-hloko ea mefuta ea lisele, lisele tsa alveolar epithelial (pampitšana e ka tlaase ea ho hema)> lisele tsa epithelial tsa moea (pampitšana e ka holimo ea ho hema)> fibroblasts, lisele tsa endothelial, le macrophages, joalo-joalo;ho tloha mofuteng oa sampole, mokelikeli oa alveolar lavage (o Khabane ka ho Fetisisa)>sekhohlela se tebileng se khohlela> nasopharyngeal swab>oropharyngeal swab>mali.Ho phaella moo, kokoana-hloko e ka boela ea fumanoa mantleng.Leha ho le joalo, ha ho nahanoa ka boiketlo ba ho buuoa le ho amoheloa ha bakuli, mohlala o sebelisoang hangata oa kliniki ke oropharyngeal swab>nasopharyngeal swab>bronchial lavage fluid (opereishene e rarahaneng) le sekhohlela se tebileng (hangata sekhohlela se omileng, seo ho leng thata ho se fumana).

Ka hona, palo ea kokoana-hloko liseleng tsa oropharynx kapa nasopharynx ea bakuli ba bang e nyenyane kapa e tlaase haholo.Haeba feela lisampole tsa oropharynx kapa nasopharynx li nkoa bakeng sa tlhahlobo, kokoana-hloko ea nucleic acid e ke ke ea fumanoa.

(2) Ha ho na lisele tse nang le kokoana-hloko tse ileng tsa bokelloa nakong ea pokello ea sampole, kapa viral nucleic acid ha ea ka ea bolokoa ka katleho.

[① Sebaka sa pokello e sa nepahalang, mohlala, ha ho bokelloa li-swabs tsa oropharyngeal, botebo ba pokello ha boa lekana, li-swabs tsa nasopharyngeal tse bokelitsoeng ha li bokelloe ka botebo ka har'a lesapo la nko, joalo-joalo.

②Sampling swabs e sebelisoa hampe.Mohlala, likhoele tsa maiketsetso tse kang PE fiber, polyester fiber le polypropylene fiber li khothaletsoa bakeng sa thepa ea hlooho ea swab.Likhoele tsa tlhaho tse kang k'hothone li sebelisoa ts'ebetsong ea sebele (ho kenngoa ka matla ha protheine 'me ha ho bonolo ho hlatsoa) Le likhoele tsa nylon (ho kenngoa ha metsi a fokolang, ho lebisang ho sampling e sa lekaneng ea molumo);

③ Tšebeliso e fosahetseng ea li-tubes tsa polokelo ea kokoana-hloko, joalo ka tšebeliso e mpe ea polypropylene kapa polyethylene polokelo ea polasetiki e bonolo ho monya nucleic acid (DNA/RNA), e leng se bakang ho fokotseha ha khatello ea nucleic acid ka har'a tharollo ea polokelo.Ha e le hantle, ho khothalletsoa ho sebelisa polasetiki ea polyethylene-propylene polymer le lijana tse ling tsa polasetiki tse entsoeng ka polypropylene ka ho khetheha ho boloka li-viral nucleic acid.]

[① Sebaka sa pokello e sa nepahalang, mohlala, ha ho bokelloa li-swabs tsa oropharyngeal, botebo ba pokello ha boa lekana, li-swabs tsa nasopharyngeal tse bokelitsoeng ha li bokelloe ka botebo ka har'a lesapo la nko, joalo-joalo.

②Sampling swabs e sebelisoa hampe.Mohlala, likhoele tsa maiketsetso tse kang PE fiber, polyester fiber le polypropylene fiber li khothaletsoa bakeng sa thepa ea hlooho ea swab.Likhoele tsa tlhaho tse kang k'hothone li sebelisoa ts'ebetsong ea sebele (ho kenngoa ka matla ha protheine 'me ha ho bonolo ho hlatsoa) Le likhoele tsa nylon (ho kenngoa ha metsi a fokolang, ho lebisang ho sampling e sa lekaneng ea molumo);

③ Tšebeliso e fosahetseng ea li-tubes tsa polokelo ea kokoana-hloko, joalo ka tšebeliso e mpe ea polypropylene kapa polyethylene polokelo ea polasetiki e bonolo ho monya nucleic acid (DNA/RNA), e leng se bakang ho fokotseha ha khatello ea nucleic acid ka har'a tharollo ea polokelo.Ha e le hantle, ho khothalletsoa ho sebelisa polasetiki ea polyethylene-propylene polymer le lijana tse ling tsa polasetiki tse entsoeng ka polypropylene ka ho khetheha ho boloka li-viral nucleic acid.]

(4) Ts'ebetso ea laboratori ea kliniki ha e lekane.Mehlala ea maemo a lipalangoang le a polokelo, ts'ebetso e tloaelehileng ea lilaboratori tsa bongaka, tlhaloso ea sephetho le taolo ea boleng ke lintlha tsa bohlokoa ho netefatsa bonnete le ts'epahalo ea liphetho tsa liteko.Ho ea ka liphetho tsa tlhahlobo ea boleng ba kantle e entsoeng ke Setsi sa Clinical Laboratory ea Komisi ea Naha ea Bophelo bo Botle ka la 16-24 Hlakubele 2020, ea lilaboratori tse 844 tse fumaneng liphetho tse nepahetseng, tse 701 (83.1%) li ne li tšoaneleha, 'me tse 143 (16.9%) li ne li sa tšoanelehe.Hoa tšoaneleha, maemo a tlhahlobo ea laboratori ka kakaretso a matle, empa lilaboratori tse fapaneng li ntse li na le phapang ea bokhoni ba ts'ebetso ea basebetsi, bokhoni bo le bong ba ho toloka mohlala o nepahetseng, le taolo ea boleng.

U ka fokotsa bobe ba bohata ba SARS-CoV-2 nucleic acid joang?

Ho fokotsa li-negative tsa bohata ho fumanoeng ha nucleic acid ho lokela ho ntlafatsoa ho tsoa likarolong tse 'nè tsa ho hlahisa li-negative tsa bohata.

(1)Ho na le tekanyo e itseng ea kokoana-hloko liseleng tsa motho ea tšoaelitsoeng.Boemo ba vaerase likarolong tse fapaneng tsa 'mele oa batho bao ho belaelloang hore ba tšoaelitsoe bo tla fapana ka linako tse fapaneng.Haeba ho se na pharynx, e ka 'na ea e-ba ka har'a mokelikeli oa bronchial lavage kapa mantle.Haeba mefuta e mengata ea lisampole e ka bokelloa ka nako e le 'ngoe kapa ka mekhahlelo e fapaneng ea tsoelo-pele ea lefu bakeng sa tlhahlobo, E tla thusa ho qoba liphoso tse fosahetseng.

(2) Lisele tse nang le kokoana-hloko li lokela ho bokelloa nakong ea ho bokella sampole.Bothata bona bo ka rarolloa haholo ka ho matlafatsa koetliso ea ba bokellang mehlala.

(3) Li-reagents tsa IVD tse ka tšeptjoang.Ka ho etsa lipatlisiso mabapi le tlhahlobo ea ts'ebetso ea ts'ebetso ea li-reagents boemong ba naha, le ho buisana ka mathata a teng, ts'ebetso ea ho lemoha ha li-reagents e ka ntlafatsoa le ho feta 'me kutloisiso ea tlhahlobo e ka ntlafatsoa.

(4) Ts'ebetso e tloaelehileng ea li-laboratories tsa kliniki.Ka ho matlafatsa koetliso ea basebetsi ba laboratori, ho tsoela pele ho ntlafatsa tsamaiso ea boleng ba laboratori, ho netefatsa likarohano tse utloahalang, le ho ntlafatsa bokhoni ba basebetsi ba ho lemoha, hoa khoneha ho fokotsa mefokolo ea bohata ka lebaka la ts'ebetso e sa lokelang ea laboratori.

Mabaka a tlhahlobo ea hape ea tlhahlobo ea SARS-CoV-2 nucleic acid ho bakuli ba hlaphohetsoeng le ba tsoileng sepetlele.

"COVID-19 Diagnosis and Treatment Plan (Trial Seventh Edition)" e hlalosa ka ho hlaka hore e 'ngoe ea mekhoa ea hore bakuli ba COVID-19 ba phekoloe le ho ntšoa sepetlele ke hore lisampole tse peli tse latellanang tsa phefumoloho li na le tlhahlobo e mpe ea nucleic acid (bonyane lihora tse 24 tse arohaneng), empa ho na le tse fokolang haholo.

(1)SARS-CoV-2 ke vaerase e ncha.Hoa hlokahala ho utloisisa ka ho eketsehileng mokhoa oa eona oa pathogenic, setšoantšo se feletseng sa lefu le bakoang le litšobotsi tsa lefu lena.Ka hona, ka lehlakoreng le leng, hoa hlokahala ho matlafatsa tsamaiso ea bakuli ba lokolotsoeng le ho etsa tlhahlobo ea bongaka ea matsatsi a 14.Etsa tatellano, tlhahlobo ea bophelo bo botle le tataiso ea bophelo bo botle ho tebisa kutloisiso ea ts'ebetso eohle ea ketsahalo, nts'etsopele le sephetho sa lefu lena.

(2)Mokuli a ka ’na a tšoaetsoa kokoana-hloko hape.Setsebi sa thuto Zhong Nanshan se itse: Hobane bakuli ba phekotsoeng ba na le li-antibodies, SARS-CoV-2 e ka felisoa ke li-antibodies ha li hlasela hape.Ho na le mabaka a mangata, e ka 'nang ea e-ba sesosa sa mokuli ea hlaphohileng, kapa e ka' na ea amana le phetoho ea kokoana-hloko, kapa sesosa sa tlhahlobo ea laboratori.Haeba e le vaerase ka boeona, phetoho ea SARS-CoV-2 e ka etsa hore antibody e hlahisoang ke mokuli ea hlaphohetsoeng e se ke ea sebetsa khahlano le vaerase e fetotsoeng.Haeba mokuli a tšoaelitsoe ke kokoana-hloko e fetotsoeng hape, tlhahlobo ea nucleic acid e ka boela ea e-ba ntle hape.

(3) Mabapi le mekhoa ea tlhahlobo ea laboratori, mokhoa o mong le o mong oa tlhahlobo o na le meeli ea ona.Ho sibolloa ha SARS-CoV-2 nucleic acid ho bakoa ke khetho ea tatellano ea liphatsa tsa lefutso, sebopeho sa li-reagents, kutloisiso ea mokhoa le mabaka a mang, a lebisang ho lisebelisoa tse teng li na le meeli ea tsona e tlase ea ho lemoha.Ka mor’a hore mokuli a phekoloe, kokoana-hloko e ’meleng ea fokotseha.Ha bongata ba kokoana-hloko sampuling e lokelang ho lekoa bo le ka tlase ho moeli o tlaase oa ho fumanoa, ho tla hlaha sephetho se "negative".Leha ho le joalo, sephetho sena ha se bolele hore kokoana-hloko e 'meleng e nyametse ka ho feletseng.Kokoana-hloko e ka ba ka mor'a hore phekolo e emisoe.Resurgence", tsoela pele ho kopitsa.Ka hona, ho kgothaletswa ho hlahloba hang ka beke nakong ea libeke tse 2 ho isa ho tse 4 ka mor'a ho tsoa.

(4) Nucleic acid ke liphatsa tsa lefutso tsa kokoana-hloko.Kokoana-hloko e bolaoa ka mor'a hore mokuli a fumane phekolo e thibelang likokoana-hloko, empa likaroloana tse setseng tsa RNA tsa kokoana-hloko li ntse li bolokiloe 'meleng oa motho,' me ha li tsoe ka ho feletseng 'meleng.Ka linako tse ling, tlas'a maemo a itseng, e ka bolokoa haholoanyane.Nako e telele, 'me ka nako ena tlhahlobo ea nucleic acid e tla ba "ea nakoana" e ntle.Ka ho atolosoa ha nako ea ho hlaphoheloa ha mokuli, ka mor'a hore likaroloana tse setseng tsa RNA 'meleng li fele butle-butle, phello ea tlhahlobo ea nucleic acid e ka fetoha e mpe.

(5) Sephetho sa tlhahlobo ea nucleic acid ea SARS-CoV-2 se paka feela boteng kapa ho ba sieo ha vaerase ea RNA, 'me e ke ke ea paka tšebetso ea vaerase le hore na vaerase ea fetisoa.Hoa hlokahala ho paka hore na mokuli ea nang le tlhahlobo e ntle ea nucleic acid hape o tla fetoha mohloli oa tšoaetso hape.Hoa hlokahala ho etsa moetlo oa vaerase ho lisampole tsa kliniki le ho hlaolela vaerase e "phelang" ho paka hore ea tšoaetsanoa.

Kakaretso

Ka bokhutšoanyane, tlhahlobo ea SARS-CoV-2 nucleic acid e etsa liteko tse fosahetseng, tse ntle hape, le maemo a mang a sa lumellaneng le lipontšo tsa kliniki a ke ke a qojoa ka botlalo.Tlhahlobong ea 'nete le litekong, ho khothaletsoa ho kopanya matšoao a kliniki, litlhahlobo tsa litšoantšo (CT) le liteko tsa laboratori (teko ea nucleic acid + tlhahlobo ea antibody e khethehileng) bakeng sa tlhahlobo e felletseng ho thibela tlhahlobo e fosahetseng le tlhahlobo e fosahetseng.Haeba liphetho tsa liteko li fumanoa li sa lumellane le lipontšo tsa kliniki, ho kgothaletswa ho etsa tlhahlobo e felletseng ea sehokelo sa tlhahlobo (mohlala oa pokello, lihokelo tsa ho potoloha le ho sebetsa) ho qhelela ka thoko tšoaetso ea pele ea vaerase ea SARS-CoV-2, ts'oaetso ea khafetsa kapa e kopantsoe le mafu a mang a kokoana-hloko ea phefumoloho, joalo-joalo.Haeba maemo a lumela, ho khothaletsoa ho bokella lisampole tse hlokolosi haholoanyane joalo ka sekhohlela kapa mokelikeli oa alveolar lavage bakeng sa tlhahlobo hape.

Lihlahisoa tse Amanang:

SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)