Dumelang, metswalle!
Ka mor'a ho etsa nucleic acid extract ka lilemo tse ngata, motho e mong le e mong o tseba hantle hore boleng ba nucleic acid extract ke senotlolo sa katleho le ho nepahala ha liteko tse latelang.
Hangata, re tsotella feela hore na karolelano ea OD260/OD280 e ka har'a mefuta e nepahetseng.Joalo ka sena…
Joalokaha polelo e bolela, u ke ke ua bua haholo, 'me u ke ke ua thaba pele ho nako.
Mamela pele Mohlophisi a u phetela palenyana!
Litšōmo tse joalo tse monate hangata li etsahala lilaboratoring tse ntšang nucleic acid.
U ne u nahana hore u nkile RNA feela, Tšoarelo!
Ha e le hantle, ke feela hore ha u tsebe hore Mong Protein o ipatile ka sekhukhu.
Na u nahana hore u batla ho etsa hore Mong Protein a hlahe ka OD260/OD280 e le 'ngoe feela?
Ke monyane haholo!Ho bonolo haholo!
Ha re sebeliseng mokhoa o bonolo ka ho fetesisa oa silevera ho o thusa ho bona liprotheine tse patiloeng ho RNA!
| Sehlahisoa se sebelisitsoeng | OD260/280 | Msesebedisoa sa ho bebofatsa |
| Foregene | 2.09 | Thermo ScientificTMNanoDropTME 'ngoe Ultra-trace UV-Vis Spectrophotometer |
| Trizol | 1.91 |
Khale khale, protheine le RNA e ne e le metsoalle e haufi.
Mong Protein o lihlong ho feta.
Kahoo haesale e le RNA e mafolofolo le e thabileng ho bapala ka eona.
Letsatsi le le leng.
RNA e ile ea bolella Monghali Protein hore e tlil'o amoheloa ke ba bang, 'me Mong Protein o ne a utloile bohloko haholo.
RNA e boetse e tsilatsila ho Mong protheine, mme a re: "Ho ka ba joang ha nka u nyenyelepa, u ipata liaparong tsa ka, u leka hore u se ke ua bonoa ke ba bang, ho lokile?"
Monghali Protein o ile a bososela ’me a lumela ka thabo.
Ka letsatsi la ho adoptha, Mong Protein o ne a hlile a ipata diaparong tsa RNA mme ha a ka a fumanwa.O ile a latela motho eo ea mo hōlisitseng ho khutlela hae ’me a phela bophelo bo thabileng ho tloha ka nako eo.
Papiso ea tlhahiso ea RNA le ForegeneSeleKakaretsoRNA Extraction Kit
le T Company's Trizol Reagent
Re ka bona ka ho hlaka lebanta la lane 4 (Trizol), ha lane 3 (Foregene) e batla e hloekile.Liphello li netefalitse hore litšila tsa protheine ea RNA tse nkiloeng ka mokhoa oa Trizol e ne e ntse e le marotholi a mangata!'Me lane 3 (Forgene) ha e na litšila tse batlang li se na protheine hobane motho ea lekang o sebelisitse lisebelisoa tsa Foregene RNA extraction!(Papatso e tlile ka tšohanyetso)
Lintlha tse ling:
https://www.foreivd.com/cell-total-rna-isolation-kit-product/
Nako ea poso: Feb-10-2022
