tlhahlobo ea asiti ea nucleic le kamore ea sampole
Sepheo sa rona e lokela ho ba ho kopanya le ho ntlafatsa boleng ba boleng bo holimo le tšebeletso ea thepa e teng, ka nako eo re atisa ho hlahisa lihlahisoa tse ncha le litharollo ho phethahatsa litlhoko tse ikhethang tsa bareki.tlhahlobo ea asiti ea nucleic le kamore ea sampole, Re ts'episa ho leka ka matla ho u fa litharollo tsa boleng bo holimo le tse sebetsang hantle.
Sepheo sa rona e lokela ho ba ho kopanya le ho ntlafatsa boleng ba boleng bo holimo le tšebeletso ea thepa e teng, ka nako eo re atisa ho hlahisa lihlahisoa tse ncha le litharollo ho phethahatsa litlhoko tse ikhethang tsa bareki.tlhahlobo ea asiti ea nucleic le kamore ea sampole, Ka lilemo tse fetang 9 tsa phihlelo le sehlopha sa litsebi, joale re rometse litharollo tsa rona linaheng tse ngata le libakeng tse ngata lefatšeng ka bophara.Re amohela bareki, mekhatlo ea likhoebo le metsoalle ho tsoa likarolong tsohle tsa lefats'e ho ikopanya le rona le ho batla tšebelisano-'moho molemong oa bobeli.
Litlhaloso
48 Rxns, 96 Rxns
Che. | Karolo | Chelete | Likarolo tse ka sehloohong | |||
48 rxn | 96 Rxns | |||||
1 | Moemeli oa ho lokolloa ha Nucleic acid | 1.4mL / tube | 2 li-tubes | 5.3 mL/botlolo | 1 botlolo | Surfactant |
2 | Tšireletso ea RNA | 27 μL / tube | 1 tube | 53 μL / tube | 1 tube | RNase inhibitor |
3 | Tharollo ea karabelo ea SARS-CoV-2 | 800 μL / tube | 1 tube | 1600 μL / tube | 1 tube | Primer, probe, buffer reaction, dNTP |
4 | Motsoako oa enzyme ea SARS-CoV-2 | 80 μL / tube | 1 tube | 160 μL / tube | 1 tube | Ho qala enzyme ea Taq, enzyme ea M-MLV |
5 | SARS-CoV-2 taolo e ntle | 100 μL / tube | 1 tube | 100 μL / tube | 1 tube | Recombinant plasmid e nang le sekhechana sa sepheo, RNA |
6 | SARS-CoV-2 taolo e mpe | 1200 μL / tube | 1 tube | 1200 μL / tube | 1 tube | TE buffer |
Likarolo le melemo
Ka potlako le bonolo.
Qoba ho lahleheloa ke nucleic acid.
Moeli o fokolang oa ho lemoha le kutloisiso e phahameng.
Tlhokahalo e tlase ea lisebelisoa le tšebeliso e tenyetsehang haholo.
Boikutlo ba ho lemoha bo ka fihla boemong bo tlaase ka ho fetisisa e le likopi tse 500/mL).
GCE/mL | Ct boleng | |
N gene | Mofuta oa ORF1ab | |
2.00E+08 | 16.43 | 15.16 |
2.00E+07 | 19.48 | 18.58 |
2.00E+06 | 22.71 | 21.86 |
2.00E+05 | 25.95 | 25.33 |
2.00E+04 | 29.30 | 28.49 |
2.00E+03 | 32.37 | 31.37 |
2.00E+02 | 35.32 | 34.47 |
2.00E+01 | 38.29 | 38.27 |
2.00E+00 | N/A | N/A |
2.00E-01 | N/A | N/A |
NC | N/A | N/A |
Linear amplification curve
Mokhoa oa ho sebelisa
1. Sesebelisoa se sebetsang
2. Mokhoa oa ho lemoha
Mohlala oa ts'ebetso: Naopharyngeal kapa oropharyngeal swab sampole (mohato o le mong feela oa ts'ebetso)(Emela metsotso e 10)
PCR amplificaton(<=55min)
Mokhoa oa ho sebetsa:
Polokelo
e tiisitsoe khanyeng 'me e bolokiloe ho -20±5℃;
Shelf bophelo:1 selemo
Sepheo sa rona e lokela ho ba ho kopanya le ho ntlafatsa boleng bo phahameng le tšebeletso ea thepa e teng, ka nako e ts'oanang re hlahisa lihlahisoa tse ncha le litharollo khafetsa ho phethahatsa litlhoko tse ikhethang tsa bareki bakeng sa fektheri e fanoeng China FDA Sterile Medical Nylon Flock Flocked Sampling Specimen Collection Throat
Fektheri e fanoeng ka China Swab, Injection Instrument, Ka lilemo tse fetang 9 tsa boiphihlelo le sehlopha sa litsebi, joale re rometse litharollo tsa rona linaheng tse ngata le libaka lefatšeng ka bophara.Re amohela bareki, mekhatlo ea likhoebo le metsoalle ho tsoa likarolong tsohle tsa lefats'e ho ikopanya le rona le ho batla tšebelisano-'moho molemong oa bobeli.