Ho theha boleng bo eketsehileng bakeng sa bareki ke filosofi ea rona ea khoebo;Ho hola ha bareki ke ts'ebetso ea rona ea ho lelekisa lisebelisoa tsa ho itšehla thajana tsa plasmid dna opereishene ea mocheso oa kamore ka potlako le ka mokhoa o bonolo, Re lumela hore sehlopha se chesehang, sa bofetoheli le se koetlisitsoeng hantle se lokela ho theha likamano tse ntle haholo tsa khoebo le uena haufinyane.Hopola hore ha u na litšenyehelo tsa ho ikopanya le rona bakeng sa lintlha tse ling.
Ho theha boleng bo eketsehileng bakeng sa bareki ke filosofi ea rona ea khoebo;ho hola ha bareki ke ts'ebetso ea rona ea ho lelekisaChina DNA Extract, Dna Ho Isolation, Ho tloha kamehla, re khomarela "ho buleha le ho hloka leeme, ho arolelana ho fumana, ho lelekisa bokhabane, le pōpo ea boleng", ho khomarela "botšepehi le mekhoa e metle, e sekametseng khoebong, tsela e molemo ka ho fetisisa, e ntle ka ho fetisisa ea valve" filosofi ea khoebo.Hammoho le rona lefats'eng lohle re na le makala le balekane ho nts'etsapele libaka tse ncha tsa khoebo, boleng bo phahameng ka ho fetesisa.Re amohela ka tieo 'me hammoho re arolelana lisebelisoa tsa lefats'e, ho bula mosebetsi o mocha hammoho le khaolo.

Litlhaloso tsa Kit

Lihlahisoa tsa FOREGENE'S nucleic acid extraction and purification series li ka ntšoa le ho hloekisoa ho li-nucleic acid tsa boleng bo holimo ho tsoa mehloling e fapaneng le lisampole tsa likarolo tse rarahaneng.Lihlahisoa tsa DNA Extractive li thehiloe holim'a DNA-Feela silica membrane purification columns le li-reagents tse ikhethang tse ka hloekisang DNA ea boleng bo phahameng ho tsoa mefuteng e fapaneng.

The General Plasmid Mini Kit e sebelisa theknoloji ea kholomo ea tlhoekiso ea DNA-Feela le mokhoa o sebetsang oa SDS lysis ho fumana plasmid ea boleng bo holimo ho libaktheria nakong ea metsotso e 20.DNA-Feela silica gel membrane e sebelisitsoeng kholomong ea spin ke ntho e ncha e ikhethang ea FOREGENE, e ka bapatsang DNA hantle le ka ho hlaka, le ho tlosa RNA, liprotheine tse sa hloekang, li-ion le metsoako e meng ea lintho tse phelang liseleng ho isa tekanyong e kholo.Boholo bo tlamang ba DNA ea plasmid ea kholomo ea tlhoekiso ea DNA-Feela ke 60μg DNA.Palo ea plasmid DNA e hlileng e nkiloeng e amana le boholo ba tharollo ea baktheria, palo ea likopi tsa plasmid (kopi e phahameng, kopi e tlaase), mathata, maemo a setso le lintlha tse ling.

Litlhaloso

Likarolo le melemo

- Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho plasmid DNA ntle le ho eketsa RNase nakong ea teko, ho qoba ho silafatsoa ke laboratori ka RNase e sa tloaelehang.

- Lebelo le potlakileng: Ho ntšoa ha Plasmid DNA ho ka phethoa nakong ea metsotso e 20.

- E Sireletsehile: Ha ho hlokahale ho ntšoa ha reagent ea organic.

- Boleng bo phahameng: Plasma e fumanoeng ka ho ntšoa ha e na RNA, ha e na RNase le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.

Sesebelisoa sa Kit

Ho ntšoa le ho hloekisa DNA ea Plasmid bakeng sa libaktheria tsa Gram-negative.

Phallo ea mosebetsi

Setšoantšo

Polokelo le shelf bophelo

Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ℃);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ka 2–8℃.

Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka bateng ea metsi ea 37 ℃ metsotso e 10 ho qhala metsi, 'me u e kopanye pele u e sebelisa.

Ho theha boleng bo eketsehileng bakeng sa bareki ke filosofi ea rona ea khoebo;ho hola ha bareki ke mosebetsi oa rona oa ho lelekisa Bahoebi ba China 96automated Nucleic Acid Extraction System for PCR Sampling Preparation, Re lumela hore sehlopha se chesehang, sa phetoho le se koetlisitsoeng hantle se ka khona ho theha likamano tsa khoebo tse ntle haholo le tse sebetsang hammoho le uena haufinyane.Hopola hore ha u na litšenyehelo tsa ho ikopanya le rona bakeng sa lintlha tse ling.
Bahoebi ba Kakaretso baChina DNA Extract, DNA Isolation, Ho tloha ka linako tsohle, re khomarela "ho buleha le ho hloka leeme, ho arolelana ho fumana, ho lelekisa bokhabane, le pōpo ea boleng", ho khomarela "botšepehi le boits'oaro bo botle, mokhoa oa khoebo, mokhoa o motle ka ho fetisisa , belofo e ntle ka ho fetisisa" filosofi ea khoebo.Hammoho le rona lefats'eng lohle re na le makala le balekane ho nts'etsapele libaka tse ncha tsa khoebo, boleng bo phahameng ka ho fetesisa.Re amohela ka tieo 'me hammoho re arolelana lisebelisoa tsa lefats'e, ho bula mosebetsi o mocha hammoho le khaolo.