RT Easyᵀᴹ I Master Premix bakeng sa motsoako oa pele oa cDNA
Tlhaloso ea Kit:
- Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 25 feela ho phethela motsoako oa cDNA ea pele.
- Sistimi e phahameng ea sensitivity reverse transcript, litempele tsa boemo ba pg le tsona li ka fumana cDNA ea boleng bo holimo.
- Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso oa karabelo ea sistimi e ka ba holimo ho 50 ℃,'me e na le ts'ebetso e ntle ea ho fetolela ka morao.
- Esita le disampole tse sa hloekang tsa RNA (joala bo fihlang ho 45%, letsoai la guanidine ho fihla ho 750mM) li ka ba tlas'a karabelo e fapaneng ea transcript.
Litlhaloso tsa Kit
FOREGENE's 2× RT EasyTMMotsoako o ka kopanya cDNA ea strand ea pele mochesong o ka holimo ho 50 ° C, e leng se loketseng karabelo ea morao-rao ea litempele tse rarahaneng tsa RNA.
RNA e hloekisitsoeng ka laboratoring hangata e na le masala a joala le letsoai la guanidine, tse nang le thepa e matla ea ho thibela ho li-reverse transcriptases, e leng se bakang liphello tse sa thabiseng tsa ho ngola reverse kapa katleho e tlaase ea ho ngola.FOREGENE 2 × RT BonoloTMMotsoako o bontša mamello e phahameng haholo ea joala le letsoai la guanidine, mamello e phahameng ka ho fetisisa ea tai ho lisampole tsa RNA ke 45%, 'me mamello e phahameng ka ho fetisisa ea letsoai la guanidine ke 750mM.Le ha RNA e sa hloeka, karabelo ea reverse transcript e ka etsoa ka FORREGENE 2 × RT Mix.Sistimi e ikhethileng ea karabelo e etsa hore karabelo ea RT e be bonolo, e potlake hape e sebetse hantle, 'me motsoako oa cDNA oa pele o ka phethoa ka metsotso e 25.
Sehlahisoa sena se ka sebelisoa hammoho le sehlahisoa sa Foregene's fluorescent quantitative Real Time PCR Easy.TMho fumana liphetho tsa liteko tsa boleng bo holimo.
Litlhaloso
| RT BonoloTMI Master Premix bakeng sa motsoako oa pele oa cDNA | ||
| Likahare tsa Kit | RT-01011 | RT-01012 |
| makhetlo a 25 (20μl system) | makhetlo a 100 (20μl system) | |
| 2× RT EasyTM Mix | 0.25ml | 1ml |
| Primer e sa fetoheng (50μM) | 50μl | 200μl |
| Oligo(dT)18 Primer (50μM) | 50μl | 200μl |
| RNase-Free ddH2O | 1.7ml | 1.7ml |
| Bukana ea Litaelo | 1 sengoathoana | 1 sengoathoana |
Likarolo le melemo
- Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 25 feela ho phethela motsoako oa cDNA ea pele.
- Sistimi e phahameng ea sensitivity reverse transcript, litempele tsa boemo ba pg le tsona li ka fumana cDNA ea boleng bo holimo.
- Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso oa karabelo ea sistimi e ka ba holimo ho 50 ℃, 'me e na le ts'ebetso e ntle ea ho fetolela ka morao.
- Esita le disampole tse sa hloekang tsa RNA (joala bo fihlang ho 45%, letsoai la guanidine ho fihla ho 750mM) li ka ba tlas'a karabelo e fapaneng ea transcript.
Sesebelisoa sa Kit
- RT-PCR e tloaelehileng
- Synthesis ea cDNA bakeng sa lipatlisiso tsa cloning le polelo
- Tshekatsheko ya mokgwa wa katoloso ya pele ya sebaka sa qalo ya dingolwa
- RACE (katoloso e potlakileng ea cDNA e qetella)
- Matlafatso ea mohala oa RNA
- Letšoao la chip
Phallo ea mosebetsi
Polokelo le shelf bophelo
1.Maemo a lipalangoang
Ts'ebetso eohle ea lipalangoang tsa li-ice box tsa mocheso o tlase, ho netefatsa hore kit e boemong ba <4 ℃.
2. Maemo a polokelo
RT BonoloTMKe bolokoa ho -20°C.Boloka sehlahisoa ka sehatsetsing se tsitsitseng sa mocheso ho -20 ° C hang ka mor'a ho fumana.Haeba maemo a polokelo a loketse, sehlahisoa se ke ke sa nyenyefatsa ts'ebetso leha e le efe nakong ea selemo sa 1 sa ho netefatsa.
