Likhopolo tse potlakileng le tse ntle haholo, baeletsi ba nang le tsebo ba tla u thusa ho khetha sehlahisoa se nepahetseng se lumellanang le litlhoko tsohle tsa hau, nako e khuts'oane ea tlhahiso, taolo e ikarabellang ea boleng bo holimo le lits'ebeletso tse ikhethang bakeng sa ho lefa le ho tsamaisa litaba tsa thepa bakeng sa theko e qotsitsoeng ea China.Click-It Edu-647Cell Proliferation Detection Kit, Khoebo ea pele, rea utloisisana.Khoebo e eketsehileng, tšepo e ea fihla moo.Feme ea rona hangata e u sebeletsa ka nako efe kapa efe.
Litheko tse potlakileng le tse ntle haholo, baeletsi ba nang le tsebo ba tla u thusa ho khetha sehlahisoa se nepahetseng se lumellanang le litlhoko tsohle tsa hau, nako e khuts'oane ea tlhahiso, taolo ea boleng bo holimo le lits'ebeletso tse ikhethileng bakeng sa ho lefa le ho tsamaisa litaba tsa thepa.China Cell Proliferation Kit, Click-It Edu-647, Lihlahisoa tsa rona li tsejoa ka ho pharaletseng ebile li tšeptjoa ke basebelisi 'me li ka kopana le liphetoho tse tsoelang pele tsa litlhoko tsa moruo le tsa sechaba.Re amohela bareki ba bacha le ba khale ba tsoang mekhoeng eohle ea bophelo ho ikopanya le rona bakeng sa likamano tsa nako e tlang tsa khoebo le katleho e kopaneng!

Litlhaloso tsa Kits

50 Preps, 200 Preps

Setsi sena se sebelisa "spin column le formula" e entsoeng ke k'hamphani ea rona, e ka ntšang RNA e hloekileng le ea boleng bo phahameng ka ho fetisisa ho tsoa ho mefuta e sa tšoaneng ea liphoofolo tse nang le bokhoni bo phahameng.E fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo le ho bapatsa DNA ea genomic ho tloha ho supernatant le lysate ea lisele, e bonolo le e bolokang nako;Kholomo ea RNA-feela e ka tlama RNA hantle 'me e ka sebetsoa ka nako e le 'ngoe ka foromo e ikhethang Mehlala e mengata.

Tsamaiso eohle ke RNase-Free, e le hore RNA e nkiloeng e se ke ea senyeha;Buffer RW1, Buffer RW2 buffer system ea ho hlatsoa, ​​e le hore RNA e fumanoeng e se na protheine, DNA, ion, le tšilafalo ea motsoako oa organic.

Likarolo tsa Kit:

Likarolo le melemo

■ Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA;tsamaiso eohle ke RNase-Free
■ Tlosa ka katleho DNA-sebelisa DNA-Cleaning Column
■ Tlosa DNA ntle le ho eketsa DNase
■ Ts'ebetso e bonolo-tsohle e phethoa ka mocheso oa kamore
■ Ts'ebetso e potlakileng e ka phethoa ka mor'a metsotso e 30
■ Sireletsehile-ha ho na reagent ea tlhaho e hlokahalang
■ Bohloeki bo phahameng -OD260/280≈1.8-2.1

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa mefuteng e fapaneng ea lithane tsa liphoofolo tse foreshe kapa tse hatselitsoeng kapa lisele tse holileng.

Lihlahisoa tsa lihlahisoa

■ Lisebelisoa tse tlase: motsoako oa pele oa cDNA, RT-PCR, cloning ea molek'hule, Northern Blot, joalo-joalo.
■ Mehlala: lisele tsa liphoofolo, lisele tse hōlileng tsebong
■ Tekanyetso: Li-tissues 10-20mg, Lisele (2-5)×10⁶
■ Boholo bo tlamang DNA ea kholomo ea tlhoekiso: 80 μg
■ Molumo oa elution: 50-200 μl

Phallo ea mosebetsi

Setšoantšo

Animal Total RNA Isolation Kit tšoaroa 20mg
Fresh mouse disampole, nka 5% hloekisitsoeng kakaretso RNA 1% agar

Glycogel electrophoresis
1: Lesapo la 2: Liphio
3: Sebete 4: Pelo

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 24 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele.Buffer Rl1 e ka bolokoa ho 4 ℃ bakeng sa ho eketsa litaba tsa hau, ke litšoaneleho tse ntle tsa ho tsamaisa - ke li-baeletsi tsa pele.Khoebo e eketsehileng, tšepo e ea fihla moo.Feme ea rona hangata e u sebeletsa ka nako efe kapa efe.
Theko e qotsitsoeng bakeng saChina Cell Proliferation Kit, Click-It Edu-647, Lihlahisoa tsa rona li tsejoa ka ho pharaletseng ebile li tšeptjoa ke basebelisi 'me li ka kopana le liphetoho tse tsoelang pele tsa litlhoko tsa moruo le tsa sechaba.Re amohela bareki ba bacha le ba khale ba tsoang mekhoeng eohle ea bophelo ho ikopanya le rona bakeng sa likamano tsa nako e tlang tsa khoebo le katleho e kopaneng!