Boleng bo ka tšeptjoang le boemo bo botle ba mokitlane ke melao-motheo ea rona, e tla re thusa ho ba maemong a holimo.Ho latela molao-motheo oa "boleng pele, moreki ea phahameng" bakeng sa theko e utloahalang China Hot Selling Good Quality Collection Kit Saliva Sample Collection Kits, Re tla fana ka thepa ea boleng bo holimo le litharollo tse ntle mefuteng ea litheko tsa tlholisano.Qala ho rua molemo litšebeletsong tsa rona tse felletseng ka ho ikopanya le rona kajeno.
Boleng bo ka tšeptjoang le boemo bo botle ba mokitlane ke melao-motheo ea rona, e tla re thusa ho ba maemong a holimo.Ho ikamahanya le maemo a "boleng pele, moreki a phahame" bakeng saChina Mathe Pokello Kit, Sekhahla sa Pokello ea Mathe se sa Hlaseheng, Khotsofalo ea bareki ke sepheo sa rona.Re labalabela ho sebelisana le uena le ho fana ka litšebeletso tsa rona tse molemohali bakeng sa hau.Re u amohela ka mofuthu hore u ikopanye le rona 'me ka kopo ikutloe u lokolohile ho ikopanya le rona.Batla kamore ea rona ea ho bonts'a marang-rang ho bona hore na re ka u etsetsa eng.Ebe u re romella lengolo-tsoibila mabapi le lintlha kapa lipotso tsa hau kajeno.

Litlhaloso

50 Preps, 200 Preps

Thepa e sebelisa "spin column le formula" e ntlafalitsoeng ke Foregene, e ka ntšang RNA ea boleng bo holimo le ea boleng bo holimo ho lithane tse fapaneng tsa semela tse nang le li-polysaccharides tse tlase le polyphenols.Bakeng sa lisampole tsa semela tse nang le li-polysaccharides tse phahameng kapa polyphenols, ho khothaletsoa ho sebelisa Plant Total RNA Isolation Plus Kit ho fumana liphetho tse ntle tsa ho ntšoa ha RNA.Kit e fana ka kholomo ea DNA-Cleaning e ka tlosang DNA ea genomic habonolo ho supernatant le lysate ea lisele.Kholomo ea RNA feela e ka tlama RNA ka nepo.Kit e ka sebetsana le palo e kholo ea lisampole ka nako e le 'ngoe.

Tsamaiso eohle ha e na RNase, kahoo RNA e hloekisitsoeng e ke ke ea senyeha.Buffer PRW1 le Buffer PRW2 e ka etsa bonnete ba hore RNA e fumanoeng ha e silafatsoe ke liprotheine, DNA, ions, le metsoako ea tlhaho.

Likarolo tsa Kit

Likarolo le melemo

■ Ts'ebetso ka mocheso oa kamore (15-25 ℃) ho pholletsa le ts'ebetso eohle, ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Complete Kit RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama ho DNA, e le hore kit e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa nakong ea metsotso e 30.
■ Tšireletseho: ha ho na reagent ea tlhaho e hlokahalang.
■ Boleng bo phahameng: Likaroloana tsa RNA tse hloekisitsoeng li hloekile haholo, ha li na protheine le litšila tse ling, 'me li ka kopana le mekhoa e fapaneng ea liteko tse tlase.

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha kakaretso ea RNA ho tloha lisampoleng tse foreshe kapa tse hatselitsoeng (haholo-holo mahlaku a mahlaku a semela) a nang le polysaccharide e tlase le polyphenol.

Phallo ea mosebetsi

Setšoantšo

Plant Total RNA Isolation Kit Plus e sebelitse 50mg ea makhasi a macha a polysaccharides le polyphenols, 'me 5% e hloekisitsoeng ea RNA e ile ea lekoa ke electrophoresis.
1: Banana
2: Ginkgo
3: K'hothone
4: Kharenate

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) sebakeng se omileng, le 2-8 ℃ nako e telele (likhoeli tse 24).

Buffer PSL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho eketsa 2-hydroxy-1-ethanethiol(optional).Boleng bo ka tšeptjoang le lintlha tse kholo tsa mokitlane ke melao-motheo ea rona, e tla re thusa boemong bo phahameng.Ho latela molao-motheo oa "boleng pele, moreki ea phahameng" bakeng sa theko e utloahalang China Hot Selling Good Quality Collection Kit Saliva Sample Collection Kits, Re tla fana ka thepa ea boleng bo holimo le litharollo tse ntle mefuteng ea litheko tsa tlholisano.Qala ho rua molemo litšebeletsong tsa rona tse felletseng ka ho ikopanya le rona kajeno.
Theko e utloahalangChina Mathe Pokello Kit, Sekhahla sa Pokello ea Mathe se sa Hlaseheng, Khotsofalo ea bareki ke sepheo sa rona.Re labalabela ho sebelisana le uena le ho fana ka litšebeletso tsa rona tse molemohali bakeng sa hau.Re u amohela ka mofuthu hore u ikopanye le rona 'me ka kopo ikutloe u lokolohile ho ikopanya le rona.Batla kamore ea rona ea ho bonts'a marang-rang ho bona hore na re ka u etsetsa eng.Ebe u re romella lengolo-tsoibila mabapi le lintlha kapa lipotso tsa hau kajeno.