Theko e tlaase ka ho Fetisisa Dnasecure Plant Kit bakeng sa DNA Extraction Nucleic Acid Purification
Tlhaloso ea Kit:
Ka potlako hloekisa DNA ea boleng bo phahameng ba genomic maling a anticoagulated (<1ml).
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
Basebetsi ba rona ka koetliso e nang le boiphihlelo.Tsebo e nang le litsebo, maikutlo a tiileng a mofani oa thepa, ho khotsofatsa litlhoko tsa bareki bakeng sa Super Lowest Price Dnasecure Plant Kit bakeng sa DNA Extraction Nucleic Acid Purification, Re lakatsa ho khetha monyetla ona oa ho netefatsa likamano tsa nako e telele tsa likhoebo tse nyane le bareki ho tsoa lefats'eng lohle.
Basebetsi ba rona ka koetliso e nang le boiphihlelo.Tsebo ea litsebo tse nang le tsebo, kutloisiso e tiileng ea mofani, ho khotsofatsa litlhoko tsa tšehetso tsa bareki bakeng saChina Nucleic Acid Extraction Reagent Kit le Kit Nucleic Acid Extraction Reagent Kit, Lihlahisoa tsa rona li tsejoa ka ho pharaletseng ebile li tšeptjoa ke basebelisi 'me li ka kopana le litlhoko tse tsoelang pele tsa moruo le tsa sechaba.Re amohela bareki ba bacha le ba khale ba tsoang mekhoeng eohle ea bophelo ho ikopanya le rona bakeng sa likamano tsa nako e tlang tsa khoebo le ho fihlela katleho e tšoanang!
Litlhaloso tsa Kit
Setsi sena se nka Foregene Protease Plus e ncha le lisebelisoa tse ikhethang tsa BL1 le BL2, tse ka khonang ho cheka lisampole tsa mali tsa anticoagulant ka nako e khuts'oane, kahoo ho qoba ho senyeha ha DNA ho isa tekanyong e kholo le ho fumana boholo ba DNA ea genomic.Sistimi e potlakileng ea ts'ebetso ea mali le ts'ebetso e bonolo ea DNA-Feela e nolofatsa haholo ho ntšoa ha genome ea mali, ho etsa hore DNA ea genomic e ka fumanoa ka boleng bo holimo le bohloeki bo phahameng nakong ea metsotso e 40.
DNA-feela silica gel membrane e sebelisitsoeng ka har'a kholomo ea centrifuge ea kit ena ke mofuta o mocha oa thepa e ikhethang ho k'hamphani ea rona, e ka tlamang DNA ka katleho le ka ho khetheha, 'me e ka tlosa RNA, litšila, liprotheine, li-ion le metsoako e meng ea lintho tse phelang liseleng ho isa tekanyong e phahameng.Likaroloana tse fumanoeng tsa DNA li kholo, bohloeki bo phahameng, bo tsitsitse ebile bo tšepahala.Bophahamo bo boholo ba kholomo ea DNA-Feela ke 80 μg DNA.DNA e fumanoeng e ka sebelisoa litekong tsa baeloji ea limolek'hule tse kang tšilo ea enzyme, PCR, hybridization ea Boroa le kaho ea laeborari.
Thepa e ka sebetsa ho fihlela ho 1ml ea mali ka nako ho fumana palo e lekaneng ea DNA ea genomic e hloekileng.
Litlhaloso
| Mali a DNA Mini Kit | |||
| Likahare tsa Kit | DE-05111 | DE-05112 | DE-05113 |
| 50 T | 100 T | 250 T | |
| Buffer BL1 | 15 ml | 30 ml | 75 ml |
| Buffer BL2 * | 15 ml | 30 ml | 75 ml |
| Buffer DC | 100 ml | 200 ml | 500 ml |
| Buffer PW * | 25 ml | 50 ml | 125 ml |
| Sekhahla sa WB1 | 15 ml | 30 ml | 75 ml |
| Buffer EB | 10 ml | 20 ml | 50 ml |
| Foregene Protease Plus | 1.25 mL | 2.5 ml | 6.5 ml |
| DNA-Feela Kholomo | 50 | 100 | 250 |
| Bukana | 1 | 1 | 1 |
*: Buffer BL2 le Buffer PW e na le letsoai la metsi le hlabang.Ka kopo, roala liatlana 'me u nke mehato e nepahetseng ea ts'ireletso ha u sebetsa.
Likarolo le melemo
-Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho qoba laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
-Lebelo le potlakileng: Foregene Protease Plus e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso e 40.
-Bonolo: The centrifugation e etsoa mocheso oa kamore, 4 ° C centrifugation kapa ethanol precipitation ea DNA ha e hlokehe.
-Tshireletso: Ha ho qotsulo ea organic reagent e sebelisoang.
-Boleng bo phahameng: Likaroloana tse nkiloeng tsa genomic DNA li kholo, ha ho na RNA, ha ho RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse fapa-fapaneng.
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisa DNA ea genomic maling a foreshe kapa a leqhoa a anticoagulated.
Phallo ea mosebetsi
Polokelo le shelf bophelo
Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho 2-8°C.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.
Tharollo ea Foregene Protease Plus e na le foromo e ikhethang, e sebetsang ha e bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);e tla ba e sebetsang haholoanyane le e tsitsitseng ha e bolokiloe ho 4 ° C, kahoo ho kgothaletswa ho e boloka ho 4 ° C, hopola hore u se ke ua e boloka ho -20 ° C.
Basebetsi ba rona ka koetliso e nang le boiphihlelo.Tsebo e nang le litsebo, maikutlo a tiileng a mofani oa thepa, ho khotsofatsa litlhoko tsa bareki bakeng sa Super Lowest Price Dnasecure Plant Kit bakeng sa DNA Extraction Nucleic Acid Purification, Re lakatsa ho khetha monyetla ona oa ho netefatsa likamano tsa nako e telele tsa likhoebo tse nyane le bareki ho tsoa lefats'eng lohle.
Theko e tlaase ka ho FetisisaChina Nucleic Acid Extraction Reagent Kit le Kit Nucleic Acid Extraction Reagent Kit, Lihlahisoa tsa rona li tsejoa ka ho pharaletseng ebile li tšeptjoa ke basebelisi 'me li ka kopana le litlhoko tse tsoelang pele tsa moruo le tsa sechaba.Re amohela bareki ba bacha le ba khale ba tsoang mekhoeng eohle ea bophelo ho ikopanya le rona bakeng sa likamano tsa nako e tlang tsa khoebo le ho fihlela katleho e tšoanang!
