Re bile moetsi ea nang le boiphihlelo.Ho hapa boholo ba litifikeiti tsa hau tsa bohlokoa tsa 'maraka oa Super Purchasing for China Real-Time PCR, Haeba u ka khahloa ke thepa efe kapa efe, hopola ho ikutloa u lokolohile ho ikopanya le rona bakeng sa lintlha tse ling kapa etsa bonnete ba hore o tla re romella lengolo-tsoibila hantle, re tla u araba ka mor'a lihora tse 24 feela.
Re bile moetsi ea nang le boiphihlelo.Ho hapa boholo ba litifikeiti tsa hau tsa bohlokoa tsa 'maraka oa eona bakeng saChina PCR, Nako ea 'Nete Pcr, Ka moea o makatsang oa" ts'ebetso e phahameng, bonolo, ts'ebetso le boqapi", 'me tumellanong le tataiso e joalo ea "boleng bo botle empa theko e betere," le "mokitlane oa lefats'e", re ikitlaelletsa ho sebelisana le lik'hamphani tsa likarolo tsa makoloi lefatšeng ka bophara ho etsa tšebelisano-'moho e atlehileng.

Litlhaloso

Setsi sena se sebelisa sistimi e ikhethileng ea lysis buffer e ka lokollang RNA kapele ho lisampole tsa lisele tse hlahisitsoeng bakeng sa karabelo ea RT-qPCR, ka hona e felisa ts'ebetso ea tlhoekiso ea RNA e jang nako le e boima.Setšoantšo sa RNA se ka fumanoa ka metsotso e 7 feela.The 5×Direct RT Mix le 2×Direct qPCR Mix-SYBR reagents e fanoeng ke kit e ka fumana kapele le ka nepo liphetho tsa nako ea nnete tsa PCR.

5 × Direct RT Mix le 2 × Direct qPCR Mix-SYBR li na le mamello e matla ea inhibitor, 'me lysate ea lisampole e ka sebelisoa e le template ea RT-qPCR ka ho toba.Setsi sena se na le RNA e phahameng ea kamano e phahameng ea Foregene reverse transcriptase, le Hot D-Taq DNA polymerase, dNTPs, MgCl.₂, buffer ea karabelo, PCR optimizer le stabilizer.

Litlhaloso

200×20μl Rxns, 1000×20μl Rxns

Likarolo tsa Kit

Likarolo le melemo

■ E bonolo ebile e sebetsa : ka theknoloji ea Cell Direct RT, lisampole tsa RNA li ka fumanoa ka metsotso e 7 feela.

■ Tlhokahalo ea sampole e nyane, e ka tlase ho lisele tse 10 li ka lekoa.

■ Phallo e phahameng: e khona ho lemoha ka potlako RNA liseleng tse entsoeng ka lipoleiti tse 384, 96, 24, 12, 6.

■ DNA Eraser e ka tlosa ka potlako liphatsa tsa lefutso tse lokollotsoeng, ea fokotsa haholo tšusumetso liphethong tse latelang tsa liteko.

■ Optimized RT le qPCR system e etsa hore mehato e 'meli ea RT-PCR reverse transcript e sebetse hantle le PCR e be e hlakileng haholoanyane, 'me e hanane le RT-qPCR reaction inhibitors.

Sesebelisoa sa Kit

Sekhahla sa ts'ebeliso: lisele tse tsoetseng pele.

- RNA e lokollotsoe ka mohlala oa lysis: e sebetsa feela ho template ea RT-qPCR ea kit ena.

- Thepa e ka sebelisoa bakeng sa merero e latelang: tlhahlobo ea polelo ea liphatsa tsa lefutso, netefatso ea siRNA-mediated gene silence effect, tlhahlobo ea lithethefatsi, joalo-joalo.

Setšoantšo

Storage le Shelf bophelo

Karolo ea I ea kit ena e lokela ho bolokoa ho 4 ℃;Karolo ea II e lokela ho bolokoa ho -20 ℃.

Foregene Protease Plus II e lokela ho bolokoa mochesong oa 4 ℃, e se ke ea hoama ho -20℃.

Reagent 2 × Direct qPCR Mix-SYBR e lokela ho bolokoa ho -20 ℃ lefifing;haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4 ℃ bakeng sa polokelo ea nako e khutšoanyane (sebelisa ka hare ho matsatsi a 10) Re bile moetsi ea nang le phihlelo.Ho hapa boholo ba litifikeiti tsa hau tsa bohlokoa tsa 'maraka oa Super Purchasing bakeng sa China Q160/Q320 Real-Time PCR, Haeba u ka khahloa ke thepa efe kapa efe, hopola ho ikutloa u lokolohile ka botlalo ho ikopanya le rona bakeng sa lintlha tse ling kapa etsa bonnete ba hore o re romelletse lengolo-tsoibila hantle, re tla u araba ka mor'a lihora tse 24 feela mme litefiso tse ntlehali li tla fanoa.
Super Purchasing bakeng saChina PCR, PCR ea Nako ea 'Nete, Ka moea o makatsang oa" ts'ebetso e phahameng, bonolo, ts'ebetso le boqapi", 'me tumellanong le tataiso e joalo ea "boleng bo botle empa theko e betere" le "mokitlane oa lefats'e", re ikitlaelletsa ho sebelisana le lik'hamphani tsa likarolo tsa makoloi lefats'eng lohle ho etsa tšebelisano-'moho e atlehileng.