"Boleng ba pele, Botšepehi e le motheo, thuso ea 'nete le phaello e kopanetsoeng" ke mohopolo oa rona, e le mokhoa oa ho hlahisa le ho phehella bokhabane bakeng sa Theko ea thekiso ea thekiso ea theko e tlaase ea China Universal DNA Rna Isolation Kit Reagents, e susumetsoang ke meaho e potlakileng ea 'maraka oa lijo le lino tse potlakileng, Re batla lihlahisoa tse rekisoang ho feta tsohle. mmoho.
"Boleng ba pele, Botšepehi e le motheo, Thuso e tiileng le phaello e kopanetsoeng" ke mohopolo oa rona, e le mokhoa oa ho hlahisa le ho phehella bokhabane bakeng saHo hloekisoa ha Nucleic Acid ka Othomathike, China Universal Rna DNA Reagents, Khamphani ea rona hona joale e na le mafapha a mangata, 'me ho na le basebetsi ba fetang 20 k'hamphaning ea rona.Re thehile lebenkele la thekiso, kamore ea lipontšo, le polokelo ea lihlahisoa.Khabareng, re ile ra ingolisa lebitso la rona.Re tiisitse tlhahlobo ea boleng ba sehlahisoa.

Tlhaloso

50 Preps, 200 Preps

Setsi sena se sebelisa "spin column le formula" e ntlafalitsoeng ke Foregene, e ka ntšang RNA ea boleng bo holimo le ea boleng bo holimo liseleng tse entsoeng ka lipoleiti tse 96, 24, 12 le 6.

Thepa e fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo supernatant le cell lysate, ho tlama le ho tlosa DNA ea genomic.Opereishene e bonolo ebile e boloka nako.

Kholomo ea RNA-feela e ka tlama RNA hantle ka foromo e ikhethang.Palo e kholo ea disampole e ka sebetswa ka nako e le nngwe.

Likarolo tsa Kit

*Ka kopo, roala liatlana 'me u nke mehato ea ts'ireletso nakong ea ts'ebetso kaha Buffer cRL1 le Buffer RW1 li na le matsoai a chaotropic a halefisang.

Likarolo le melemo

■ Tshebetso eohle e sebetsa mocheso oa kamore (15-25 ℃), ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Thepa eohle ha e na RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa ka metsotso e 11.
■ Polokeho: Ha ho hlokahale reagent ea tlhaho.
■ Boleng bo phahameng: RNA e hloekisitsoeng e hloekile haholo, ha e na protheine le litšila tse ling, ’me e ka kopana le liteko tse sa tšoaneng tse latelang.

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa liseleng tse hlahisitsoeng ka har'a lipoleiti tse 96, 24, 12 le 6 tsa seliba.

Phallo ea mosebetsi

Setšoantšo

Sets'oants'o sa betri ea gel ea agarose ea Cell Total RNA Isolation Kit se tšoere linomoro tse kaholimo tse fapaneng tsa lisele, 20μl bophahamo ba modumo, nka 2μl e hloekisitsoeng RNA 1%.

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele (likhoeli tse 24).

Buffer cRL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho eketsa 2-hydroxy-1-ethanethiol(khetho)."Boleng ba pele, Botšepehi e le motheo, Thuso e tiileng le phaello e kopanetsoeng" ke mohopolo oa rona, e le mokhoa oa ho hlahisa le ho phehella bokhabane ba Theko ea Total China Cost-Effective Effective Building from the Universal Manufacturer Manufacturer DNA Manufacturer Renasfluent Manufacturer. ea lijo tse potlakileng le lino tse jeoang lefatšeng ka bophara , Re batla ho sebetsa le balekane / bareki bakeng sa ho etsa litholoana tse ntle hammoho.
Theko ea thekisoChina Universal Rna DNA Reagents, Ho hloekisoa ha Nucleic Acid ka Othomathike, Khamphani ea rona hona joale e na le mafapha a mangata, 'me ho na le basebetsi ba fetang 20 k'hamphaning ea rona.Re thehile lebenkele la thekiso, kamore ea lipontšo, le polokelo ea lihlahisoa.Khabareng, re ile ra ingolisa lebitso la rona.Re tiisitse tlhahlobo ea boleng ba sehlahisoa.