Lethathamo la Theko e tlase bakeng sa China RNA Isolation Kit le CE
Re tsitlallela ka khopolo ea kholo ea 'Phahamiso e phahameng ka ho fetisisa, Ts'ebetso, Botšepehi le mokhoa oa ho sebetsa oa fatše' ho u fa k'hamphani e kholo ea ho sebetsana le Lethathamo la Theko e Theko bakeng sa China RNA Isolation Kit with CE, Re ananela potso ea hau 'me ke tlotla ea rona ho sebetsa le motsoalle e mong le e mong lefatšeng ka bophara.
Re tsitlallela ka khopolo ea kholo ea 'Tlhaloso e phahameng ka ho fetisisa, Ts'ebetso, Botšepehi le mokhoa oa ho sebetsa oa fatše' ho u fa k'hamphani e kholo ea ho sebetsa.China RNA Extraction Kit, Rna Isolation Kit, Mehaho ea motheo e matla ke tlhoko ea mokhatlo ofe kapa ofe.Re tšehelitsoe ke setsi se matla sa meralo se re nolofalletsang ho etsa, ho boloka, ho hlahloba boleng le ho romella lihlahisoa tsa rona le litharollo lefatšeng ka bophara.Ho boloka tšebetso e tsamaea hantle, re arotse litšebeletso tsa rona ka mafapha a mangata.Mafapha ana kaofela a sebetsa ka lisebelisoa tsa morao-rao, mechini le lisebelisoa tsa sejoale-joale.Ka lebaka leo, re khona ho etsa tlhahiso e ngata ntle le ho sekisetsa boleng.
Litlhaloso tsa Kits
50 Preps, 200 Preps
Setsi sena se sebelisa "spin column le formula" e entsoeng ke k'hamphani ea rona, e ka ntšang RNA e hloekileng le ea boleng bo phahameng ka ho fetisisa ho tsoa ho mefuta e sa tšoaneng ea liphoofolo tse nang le bokhoni bo phahameng.E fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo le ho bapatsa DNA ea genomic ho tloha ho supernatant le lysate ea lisele, e bonolo le e bolokang nako;Kholomo ea RNA-feela e ka tlama RNA hantle 'me e ka sebetsoa ka nako e le 'ngoe ka foromo e ikhethang Mehlala e mengata.
Tsamaiso eohle ke RNase-Free, e le hore RNA e nkiloeng e se ke ea senyeha;Buffer RW1, Buffer RW2 buffer system ea ho hlatsoa, e le hore RNA e fumanoeng e se na protheine, DNA, ion, le tšilafalo ea motsoako oa organic.
Likarolo tsa Kit:
| Buffer RL1 , Buffer RL2 |
| Buffer RW1, Buffer RW2 |
| RNase-Free ddH2O, DNA-Cleaning Column |
| RNA-Feela Kholomo |
Likarolo le melemo
■ Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA;tsamaiso eohle ke RNase-Free
■ Tlosa ka katleho DNA-sebelisa DNA-Cleaning Column
■ Tlosa DNA ntle le ho eketsa DNase
■ Ts'ebetso e bonolo-tsohle e phethoa ka mocheso oa kamore
■ Ts'ebetso e potlakileng e ka phethoa ka mor'a metsotso e 30
■ Sireletsehile-ha ho na reagent ea tlhaho e hlokahalang
■ Bohloeki bo phahameng -OD260/280≈1.8-2.1
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa mefuteng e fapaneng ea lithane tsa liphoofolo tse foreshe kapa tse hatselitsoeng kapa lisele tse holileng.
Lihlahisoa tsa lihlahisoa
■ Lisebelisoa tse tlase: motsoako oa pele oa cDNA, RT-PCR, cloning ea molek'hule, Northern Blot, joalo-joalo.
■ Mehlala: lisele tsa liphoofolo, lisele tse hōlileng tsebong
■ Tekanyetso: Li-tissues 10-20mg, Lisele (2-5)×106
■ Boholo bo tlamang DNA ea kholomo ea tlhoekiso: 80 μg
■ Molumo oa elution: 50-200 μl
Phallo ea mosebetsi
Setšoantšo
Animal Total RNA Isolation Kit tšoaroa 20mg
Fresh mouse disampole, nka 5% hloekisitsoeng kakaretso RNA 1% agar
Glycogel electrophoresis
1: Lesapo la 2: Liphio
3: Sebete 4: Pelo
Polokelo le shelf bophelo
Thepa e ka bolokoa likhoeli tse 24 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele.Buffer RL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli ea 1 ka mor'a ho eketsa β- mercaptoethanol (ho ikhethela).
Re tsitlallela ka khopolo ea kholo ea 'Phahamiso e phahameng ka ho fetisisa, Performance, True and Down-to-earth working approach' ho u fa k'hamphani e kholo ea ho sebetsa bakeng sa theko e tlaase ea PriceList bakeng sa China 96t DNA Isolation Kit with CE le FDA, Re ananela potso ea hau 'me ke tlotla ea rona ho sebetsa le motsoalle e mong le e mong lefatšeng ka bophara.
Cheap PriceList bakeng sa China DNA Extraction Kit, DNA Isolation Kit, Meaho ea motheo e matla ke tlhoko ea mokhatlo ofe kapa ofe.Re tšehelitsoe ke setsi se matla sa meralo se re nolofalletsang ho etsa, ho boloka, ho hlahloba boleng le ho romella lihlahisoa tsa rona le litharollo lefatšeng ka bophara.Ho boloka tšebetso e tsamaea hantle, re arotse litšebeletso tsa rona ka mafapha a mangata.Mafapha ana kaofela a sebetsa ka lisebelisoa tsa morao-rao, mechini le lisebelisoa tsa sejoale-joale.Ka lebaka leo, re khona ho etsa tlhahiso e ngata ntle le ho sekisetsa boleng.
