Re ikemiselitse ho fana ka litšebeletso tse bonolo, tse bolokang nako le ho boloka chelete hang-hang bakeng sa bareki.Ho bonolo ho sebelisa CELL TOTAL RNA IsolATION KIT, Re amohela ka mofuthu bo-mphato ba likhoebo tse nyane ho tsoa mekhoeng eohle ea bophelo, re tšepa ho theha khoebo e nang le botsoalle le tšebelisano 'moho ho ikopanya le uena le ho fihlela sepheo sa katleho.
Re ikemiselitse ho fana ka litšebeletso tse bonolo, tse bolokang nako le ho boloka chelete hang-hang bakeng sa bareki.Ho bonolo ho sebelisa CELL TOTAL RNA IsolATION KIT, joale re lebelletse tšebelisano-'moho e kholoanyane le bareki ba mose ho maoatle ho ipapisitse le melemo e phelisanang.Re tla sebetsa ka pelo eohle ho ntlafatsa thepa le litšebeletso tsa rona.Hape re ts'episa ho sebetsa 'moho le balekane ba khoebo ho phahamisetsa tšebelisano ea rona boemong bo phahameng le ho arolelana katleho hammoho.Re u amohela ka mofuthu hore u etele fektheri ea rona ka tieo.

Litlhaloso

50 Preps, 200 Preps

Likarolo tsa Kit

Likarolo le melemo

■ Tshebetso eohle e sebetsa mocheso oa kamore (15-25 ℃), ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Thepa eohle ha e na RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa ka metsotso e 11.
■ Polokeho: Ha ho hlokahale reagent ea tlhaho.
■ Boleng bo phahameng: RNA e hloekisitsoeng e hloekile haholo, ha e na protheine le litšila tse ling, ’me e ka kopana le liteko tse sa tšoaneng tse latelang.

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa liseleng tse hlahisitsoeng ka har'a lipoleiti tse 96, 24, 12 le 6 tsa seliba.

Phallo ea mosebetsi

Setšoantšo

Sets'oants'o sa betri ea gel ea agarose ea Cell Total RNA Isolation Kit se tšoere linomoro tse kaholimo tse fapaneng tsa lisele, 20μl bophahamo ba modumo, nka 2μl e hloekisitsoeng RNA 1%.

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) le 2-8 ℃ nako e telele (likhoeli tse 24).

Buffer cRL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho eketsa 2-hydroxy-1-ethanethiol(optional).Re ikemiselitse ho fana ka litšebeletso tse bonolo, tse bolokang nako le ho boloka chelete e le 'ngoe ea bareki bakeng sa thekiso e chesang ea China Robenidine Hydrochloride Premix Molemo ka ho Fetisisa oa Selling Feed Ingredient, Re etsa metsoalle ka mofuthu ho tloha khoebong e nyenyane le ho theha mekhoa e metle ea bophelo. le ho fihlela sepheo sa win-win.
Thekiso e chesang ea China Feed Additive, Feed Grade, joale re lebelletse tšebelisano-'moho e kholoanyane le bareki ba mose ho maoatle ho latela melemo e tšoanang.Re tla sebetsa ka pelo eohle ho ntlafatsa thepa le litšebeletso tsa rona.Hape re ts'episa ho sebetsa 'moho le balekane ba khoebo ho phahamisetsa tšebelisano ea rona boemong bo phahameng le ho arolelana katleho hammoho.Re u amohela ka mofuthu hore u etele fektheri ea rona ka tieo.