Re bile motlotlo ka khotsofalo ea hau ea bareki le kamohelo e pharaletseng ka lebaka la ho phehella ha rona ho fumana boleng bo holimo ka bobeli ka ho lekana tharollong le ts'ebeletso bakeng sa mokhoa o potlakileng oa ho itšehla thajana le ho hloekisoa ha RNA ho tsoa liseleng, Ho latela filosofi ea khoebo e nyane ea 'customer 1st forge ahead', re amohela ka tieo bareki ho tsoa ntlong ea hau le ho sebelisana le rona mose ho maoatle.
Re bile motlotlo ka khotsofalo ea hau ea bareki le kamohelo e pharaletseng ka lebaka la ho phehella ha rona ho fumana boleng bo holimo ka ho lekana mabapi le tharollo le tšebeletso eamokhoa o potlakileng oa ho itšehla thajana le ho hloekisoa ha RNA e felletseng ho tsoa liseleng tsa setso, Khamphani ea rona e sebetsa ho latela molao-motheo oa ts'ebetso ea "tšebelisano-'moho e thehiloeng ho botšepehi, tšebelisano-'moho, e shebaneng le batho, tšebelisano-'moho ea katleho".Re tšepa hore re ka ba le likamano tsa botsoalle le mohoebi ea tsoang lefats'eng lohle.

Litlhaloso

50 Preps, 200 Preps

Likarolo tsa Kit

Likarolo le melemo

■ Tshebetso eohle e sebetsa mocheso oa kamore (15-25 ℃), ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Thepa eohle ha e na RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa ka metsotso e 11.
■ Polokeho: Ha ho hlokahale reagent ea tlhaho.
■ Boleng bo phahameng: RNA e hloekisitsoeng e hloekile haholo, ha e na protheine le litšila tse ling, ’me e ka kopana le liteko tse sa tšoaneng tse latelang.

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa liseleng tse hlahisitsoeng ka har'a lipoleiti tse 96, 24, 12 le 6 tsa seliba.

Phallo ea mosebetsi

Setšoantšo

Sets'oants'o sa betri ea gel ea agarose ea Cell Total RNA Isolation Kit se tšoere linomoro tse kaholimo tse fapaneng tsa lisele, 20μl bophahamo ba modumo, nka 2μl e hloekisitsoeng RNA 1%.

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) le 2-8 ℃ nako e telele (likhoeli tse 24).

Buffer cRL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho eketsa 2-hydroxy-1-ethanethiol(optional).Re 'nile ra motlotlo ka khotsofalo ea hau ea moreki le kamohelo e pharaletseng ka lebaka la ho phehella ha rona ho phehella boleng ba boleng bo phahameng ka ho lekana ka tharollo le tšebeletso bakeng sa Nako e Khutšoanyane ea Nako bakeng sa China Lilemo tse 2 Warrantying Industrial Coating Ovenst Bus Adheraying Industrial Coating, Philosomer Bus. forge ahead', re amohela bareki ka tieo ho tsoa ntlong ea hau le mose ho maoatle ho sebelisana le rona.
Nako e Khutšoane ea ho etella pele bakeng sa China Powder Coating Booth, Industrial Coating, Khamphani ea rona e sebetsa ho latela molao-motheo oa ts'ebetso ea "botšepehi, tšebelisano-'moho e thehiloeng, e shebaneng le batho, tšebelisano-'moho ea katleho".Re tšepa hore re ka ba le likamano tsa botsoalle le mohoebi ea tsoang lefats'eng lohle.