ForeSNP Genotyping Kit
Tlhaloso ea Kit:
Ho nepahala ho phahameng: ho thaepa taolo e nepahetseng, sekhahla sa ho nepahala se feta 98%.
Theko e tlase: Ha ho na tlhoko ea ho kopanya palo e kholo ea li-probes tse turang tse nang le mabitso a mabeli.
Sistimi e ntlafalitsoeng ea PCR: botsitso bo botle ba sistimi le ho nepahala ho matla ho holisa.
Sistimi ea PCR e khahlanong le tšilafalo: E ka felisa ka katleho tšilafalo ea aerosol e bakoang ke lihlahisoa tsa PCR, ntle le ho tšoenyeha ka ho kena-kenana le tšilafalo ea tikoloho ka lets'oao la fluorescent la taolo e mpe, le ho netefatsa ho nepahala ha amplification le ho nepahala ha ho thaepa.
Litlhaloso
Theknoloji ea Competitive Allele Specific PCR (Competitive Allele Specific PCR) ke mofuta o mocha oa mokhoa oa ho thaepa oa allele.Mokhoa ona ha o hloke ho etsa li-probes tse khethehileng bakeng sa SNP le inDel ka 'ngoe, empa o hloka feela lipara tse peli tsa li-probes tse ikhethang tsa bokahohleng ho fihlela mofuta o nepahetseng oa lisampole tsa genomic DNA.Ka ho sekaseka matla le karo-karolelano ea lets'oao la ho qetela la fluorescence, genotype e ikemisetsoa ka bo eona, 'me phello ea ho kopanya e bonts'oa ka pono.Mokhoa ona o na le nako e khuts'oane ea ho lemoha, litšenyehelo tse tlase tsa reagent, ho nepahala ho holimo, 'me o ka sebelisoa bakeng sa ho ikatisa ka thuso ea limolek'hule, ho beha QTL, ho tsebahatsa matšoao a lefutso le liteko tse ling tsa baeloji ea limolek'hule tse nang le molumo o moholo oa sampole.
Litlhaloso
5ml, 50ml, 50ml×10, 500ml×20
Likarolo tsa Kit
| 2 × GT e BonoloTMKopanya |
| DNase-Free ddH2O |
| Litaelo |
Likarolo le melemo
■Ho nepahala ho phahameng: ho thaepa taolo e nepahetseng, sekhahla sa ho nepahala se feta 98%.
■ Theko e tlase: Ha ho hlokahale ho kopanya palo e kholo ea li-probe tse nang le mabitso a mabeli a theko e boima.
■ Sistimi ea PCR e ntlafalitsoeng: botsitso bo botle ba sistimi le ho nepahala ho matla ho holisa.
■ Sistimi ea PCR e khahlanong le tšilafalo: E ka felisa ka katleho tšilafalo ea aerosol e bakoang ke lihlahisoa tsa PCR, ntle le ho tšoenyeha ka ho kena-kenana le tšilafalo ea tikoloho ka lets'oao la fluorescent la taolo e mpe, le ho netefatsa ho nepahala ha amplification le ho nepahala ha ho thaepa.
Sesebelisoa sa kit
E reretsoe ho sebelisoa litekong tsa genotyping tsa disampole tse hloekisitsoeng tsa DNA.
Mohlala
Tekong ena, 200ng wheat genomic DNA e sebelisitsoe e le template ho lemoha ho thaepa ha mofuta oa TaGS-D1 o amanang le boima ba lijo-thollo tsa koro tlas'a Foresnp Genotyping Kit.Mohlala oa lisebelisoa ke BIO-RAD CFX connect;palo ea lisampole ke 21, palo ea li-NTC ke 8, 'me mofuta o mong le o mong oa taolo e ntle ke 1.
ForeSNP Genotyping Kit
