Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent(zebrafish)
Litlhaloso
Sehlahisoa sena se sebelisa mokhoa o ikhethileng oa lysis buffer system ho lokolla ka potlako DNA ea genomic ho liqoaha le lithane tse ling tsa tlhapi ea metsi a hloekileng, mapheoana a mohatla kapa lisampole tsa mahe a tlhapi bakeng sa karabelo ea PCR, ka hona e loketse haholo tlhahlobo e kholo ea liphatsa tsa lefutso.Mokhoa oa ho lokolla DNA ea genomic ho tswa ho lysis buffer e phethoa ka hare ho metsotso e 10-30 ho 65 ° C.Ha ho na mekhoa e meng e kang ho tlosoa ha protheine le RNA e hlokahalang, 'me DNA ea trace e lokolotsoeng e ka sebelisoa e le template bakeng sa karabelo ea PCR.
2 × PCR e bonoloTMMix e na le mamello e matla ho PCR reaction inhibitors, 'me e ka sebelisa lysate ea sampole ho lekoa e le template bakeng sa ho holisa ka nepo le ka nepo.Reagent ena e na le ForegeneD-Taq DNAPolymerase, dNTPs, MgCl.2, buffer ea karabelo, PCR optimizer le stabilizer.E sebelisoa hammoho le lysis buffer ka potlako le ha bonolo ho lemoha disampole, 'me e na le litšobotsi tse phahameng nahanela, ho khetheha e matla, le botsitso bo botle.
D-Taq DNA polymerase ke DNA polymerase e entsoeng ka ho khetheha ke Foregene bakeng sa karabelo e tobileng ea PCR.D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho holisa DNA ka mokhoa o nepahetseng lits'ebetsong tse fapaneng tse rarahaneng tsa karabelo, mme lebelo la amplification le ka fihla ho 2Kb/min, e loketseng ka ho khetheha bakeng sa karabelo ea Direct PCR.
Litlhaloso
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Likarolo tsa Kit
Karolo ea I | Buffer FP |
Foregene Protease | |
6× DNA Loading Buffer | |
Karolo ea II | 2 × PCR e bonoloTMKopanya |
Litaelo |
Likarolo le melemo
■Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.
■ Tlhokahalo ea sampole e nyane, joalo ka ha 1mg tlhapi ea caudal fin kapa mahe a 10 a tlhapi a ka lekoa.
■ Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, ’me ho buuoa ho bonolo.
■ Sistimi e ntlafalitsoeng ea PCR e etsa hore PCR e be le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
Litekanyetso tsa kit
Kopo: Litlhapi tsa Zebra le litlhapi tse ling tsa metsi a hloekileng.
DNA e lokollotsoeng ho tsoa ho sampole lysis: e sebelisoa feela joalo ka template ea PCR.
Thepa e ka sebelisoa bakeng sa merero e latelang: ho tsebahatsa transgenosis, genotyping ea liphoofolo, joalo-joalo.
Phallo ea mosebetsi
Storage le Shelf bophelo
Karolo ea I ea kit ena e lokela ho bolokoa ho 2-8 ℃.
Reagent Buffer FP e ka bolokoa likhoeli tse 12 tlas'a maemo a omileng;e ka bolokoa ho 2-8 ℃ bakeng sa polokelo e telele.
v Reagent Foregene Protease e na le foromo e ikhethang.Ho netefatsa ts'ebetso ea eona le botsitso, ka kopo e boloke ho 4 ℃ bakeng sa likhoeli tse 12.
vReagent 6 × DNA Loading Buffer e ka bolokoa ho 4 ℃ kapa -20 ℃ nako e telele.
Karolo ea II ea kit ena e lokela ho bolokoa ho -20 ℃.
vReagent 2 × PCR EasyTM Mix, haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4 ℃ bakeng sa polokelo ea nako e khutšoanyane (sebelisa ho fihlela matsatsing a 10).