Mohlala oa mahala bakeng sa China High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2
Tlhaloso ea Kit:
◮Kit ea mohato o le mong e etsa transcript le qPCR karabelo e 'meli ka har'a tube e le' ngoe, e hloka feela ho eketsa template ea RNA, li-primer tsa PCR tse ikhethileng le RNase-Free ddH.2O.
◮Thepa e ka hlahloba RNA ea vaerase kapele le ka mokhoa o nepahetseng kapa ea lata RNA.
◮Setsi sena se sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
◮Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
◮RT-qPCR e bonoloTM(Mohato o le mong) -SYBR Green I kit e tla le dae ea ka hare ea ROX, e ka sebelisetsoang ho tlosa semelo sa matšoao le liphoso tsa matšoao lipakeng tsa liliba, tse loketseng bareki ho li sebelisa mefuteng e fapaneng ea lisebelisoa tsa PCR tse ngata.
Re lumela ho: Boqapi ke moea le moea oa rona.Boemo bo phahameng ke bophelo ba rona.Moreki o hloka ho ba le Molimo oa rona bakeng sa sampole ea mahala bakeng sa China High Sensitivity DNA/Rna One-Step Probe Rt-QpcrKit V2, Ka har'a boikitlaetso ba rona, re se re na le mabenkele a mangata Chaena mme tharollo ea rona e hapile thoriso ho tsoa ho bareki lefatšeng ka bophara.Amohela bareki ba bacha le ba siiloeng ke nako ho ikopanya le rona bakeng sa likamano tsa nako e telele tsa khoebo.
Re lumela ho: Boqapi ke moea le moea oa rona.Boemo bo phahameng ke bophelo ba rona.Moreki lokela ho ba le ke Molimo oa rōna bakeng saChina Taq DNA Polymerase, Qpcr, Ho sebetsa ka thata ho tsoela pele ho hatela pele, ho nchafatsa indasteri, ho etsa boiteko bo bong le bo bong ho khoebo ea maemo a pele.Re leka ka hohle kamoo re ka khonang ho haha mohlala oa tsamaiso ea saense, ho ithuta tsebo e ngata ea setsebi, ho hlahisa lisebelisoa tse tsoetseng pele tsa tlhahiso le mokhoa oa tlhahiso , ho etsa lihlahisoa tsa boleng ba pele, theko e utloahalang , boleng bo phahameng ba tšebeletso , ho fana ka potlako , ho u fa boleng bo bocha.
Litlhaloso
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Likarolo tsa Kit
| Karolo ea I | Buffer P1 |
| Buffer P2 | |
| 6×DNA Loading Buffer | |
| Karolo ea II | 2 × Lekhasi la PCR le BonoloTMMotsoako(UNG) |
Likarolo le melemo
■ Ha ho tlhoekiso ea DNA e jang nako le e theko e boima
■ Boitsebiso bo fokolang
■ E bonolo-sampole e ka ba tlas'a PCR reaction kapa lysis reaction ntle le ho seha kapa ho sila
■ 2 × Mix, ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi
■ Tokisetso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa bonyane ka metsotso e 50.
■ Boitšoaro bo phahameng ba throughput-lysis bo ka phethoa ka poleiti ea PCR ea seliba sa 96
Litekanyetso tsa kit
Kopo: boitsebiso bo fetotsoeng ka liphatsa tsa lefutso, genotyping, joalo-joalo.
Mohlala: lekhasi la semela
Tekanyo: Diameter 2-3mm (mokhoa o tobileng), bophara ba 5-7mm (mokhoa oa lysis)
Sebaka sa ho lemoha: sekhechana sa sepheo ≤1kb
Phallo ea mosebetsi
Electropherogram
Libeke tse peli tsa ho lemoha liphetho tsa PCR Mix tlas'a boemo ba tšilafalo ea sehlahisoa sa PCR.Re lumela ho: Boqapi ke moea le moea oa rona.Boemo bo phahameng ke bophelo ba rona.Ho hlokahala hore bareki ba be le ke Molimo oa rona bakeng sa sampole ea Mahala bakeng sa China High Sensitivity DNA/Rna One-Step Probe Rt-Qpcr Kit V2, Ka har'a matsapa a rona, re se re na le mabenkele a mangata Chaena mme tharollo ea rona e hapile thoriso ho tsoa ho bareki lefatšeng ka bophara.Amohela bareki ba bacha le ba siiloeng ke nako ho ikopanya le rona bakeng sa likamano tsa nako e telele tsa khoebo.
Free sampole bakeng saChina Taq DNA Polymerase, Qpcr, Ho sebetsa ka thata ho tsoela pele ho hatela pele, ho nchafatsa indasteri, ho etsa boiteko bo bong le bo bong ho khoebo ea maemo a pele.Re leka ka hohle kamoo re ka khonang ho haha mohlala oa tsamaiso ea saense, ho ithuta tsebo e ngata ea setsebi, ho hlahisa lisebelisoa tse tsoetseng pele tsa tlhahiso le mokhoa oa tlhahiso , ho etsa lihlahisoa tsa boleng ba pele, theko e utloahalang , boleng bo phahameng ba tšebeletso , ho fana ka potlako , ho u fa boleng bo bocha.
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