Boleng bo phahameng ba RNA
Ka ho sebelisa lenaneo le felletseng la taolo ea boleng bo holimo ba mahlale, boleng bo holimo bo holimo le tumelo e phahameng, re fumana botumo bo botle mme ra lula indastering ena bakeng saBoleng bo phahameng ba RNA, Molao-motheo oa rona ke "Litheko tse loketseng, nako ea tlhahiso ea moruo le tšebeletso e molemo ka ho fetisisa" Re tšepa ho sebelisana le bareki ba bangata bakeng sa ho matlafatsana le melemo.
Ka ho sebelisa lenaneo le felletseng la taolo ea boleng bo holimo ba mahlale, boleng bo holimo bo holimo le tumelo e phahameng, re fumana botumo bo botle mme ra lula indastering ena bakeng saBoleng bo phahameng ba RNA, Khamphani ea rona e nka "litheko tse loketseng, boleng bo holimo, nako e sebetsang ea tlhahiso le ts'ebeletso e ntle ea kamora ho rekisa" joalo ka molao oa rona.Re tšepa ho sebelisana le bareki ba bangata bakeng sa nts'etsopele le melemo nakong e tlang.Rea u amohela ho ikopanya le rona.
Litlhaloso
50 Preps, 200 Preps
Likarolo tsa Kit
| Buffer cRL1, Buffer cRL2 |
| Buffer RW1, Buffer RW2 |
| RNase-Free ddH2O, DNA-Cleaning Column |
| RNA-Feela Kholomo |
Likarolo le melemo
■ Tshebetso eohle e sebetsa mocheso oa kamore (15-25 ℃), ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Thepa eohle ha e na RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa ka metsotso e 11.
■ Polokeho: Ha ho hlokahale reagent ea tlhaho.
■ Boleng bo phahameng: RNA e hloekisitsoeng e hloekile haholo, ha e na protheine le litšila tse ling, ’me e ka kopana le liteko tse sa tšoaneng tse latelang.
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa liseleng tse hlahisitsoeng ka har'a lipoleiti tse 96, 24, 12 le 6 tsa seliba.
Phallo ea mosebetsi
Setšoantšo
Sets'oants'o sa betri ea gel ea agarose ea Cell Total RNA Isolation Kit se tšoere linomoro tse kaholimo tse fapaneng tsa lisele, 20μl bophahamo ba modumo, nka 2μl e hloekisitsoeng RNA 1%.
Polokelo le shelf bophelo
Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) le 2-8 ℃ nako e telele (likhoeli tse 24).
Buffer cRL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho eketsa 2-hydroxy-1-ethanethiol(optional).Ho sebelisa lenaneo le feletseng la tsamaiso ea boleng bo phahameng ba saense, boleng bo phahameng le tumelo e phahameng, re fumana botumo bo botle' me ra hapa indasteri ena bakeng sa Quality Quality for China Longyu Fana ka Nomilin ea Boleng bo Tšepahalang. ntlafatso le melemo.
Boleng bo Phahameng ba China Nomilin, Solvent Extraction, Khamphani ea rona e nka "litheko tse loketseng, boleng bo holimo, nako e sebetsang ea tlhahiso le ts'ebeletso e ntle ea kamora ho rekisa" joalo ka molao oa rona.Re tšepa ho sebelisana le bareki ba bangata bakeng sa nts'etsopele le melemo nakong e tlang.Rea u amohela ho ikopanya le rona.
