Ntlafatso ea rona e itšetlehile ka lisebelisoa tse phahameng, litalenta tse babatsehang le matla a theknoloji a tsoelang pele a matlafalitsoeng bakeng santsa RNA kaofela, Ho ithuta haholoanyane ka seo re ka u etsetsang sona, bua le rona neng kapa neng.Re labalabela ho theha mekhatlo e metle le ea nako e telele ea mokhatlo hammoho le uena.
Ntlafatso ea rona e itšetlehile ka lisebelisoa tse phahameng, litalenta tse babatsehang le matla a theknoloji a tsoelang pele a matlafalitsoeng bakeng santsa RNA kaofela, E sebelisa tsamaiso ea pele ea lefats'e bakeng sa ts'ebetso e ka tšeptjoang, tekanyo e tlaase ea ho hlōleha, e loketse khetho ea bareki ba Argentina.Khamphani ea rona e lutse ka har'a litoropo tsa naha tse tsoetseng pele, sephethephethe se bonolo haholo, maemo a ikhethang a sebaka le moruo.Re hahamalla tlhahiso e shebaneng le batho, ka bokhabane, ho fana ka maikutlo, ho haha ​​​​filosofi ea khoebo e ntle haholo.Tsamaiso e thata ea boleng, tšebeletso e phethahetseng, theko e loketseng Argentina ke boemo ba rona molemong oa tlholisano.Haeba ho hlokahala, amohela ho ikopanya le rona ka sebaka sa rona sa marang-rang kapa therisano ea mohala, re tla thabela ho u sebeletsa.

Litlhaloso

50 Preps, 200 Preps

Likarolo tsa Kit

Likarolo le melemo

■ Tshebetso eohle e sebetsa mocheso oa kamore (15-25 ℃), ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Thepa eohle ha e na RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa ka metsotso e 11.
■ Polokeho: Ha ho hlokahale reagent ea tlhaho.
■ Boleng bo phahameng: RNA e hloekisitsoeng e hloekile haholo, ha e na protheine le litšila tse ling, ’me e ka kopana le liteko tse sa tšoaneng tse latelang.

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa liseleng tse hlahisitsoeng ka har'a lipoleiti tse 96, 24, 12 le 6 tsa seliba.

Phallo ea mosebetsi

Setšoantšo

Sets'oants'o sa betri ea gel ea agarose ea Cell Total RNA Isolation Kit se tšoere linomoro tse kaholimo tse fapaneng tsa lisele, 20μl bophahamo ba modumo, nka 2μl e hloekisitsoeng RNA 1%.

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) le 2-8 ℃ nako e telele (likhoeli tse 24).

Buffer Crl1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho eketsa lisebelisoa tsa matsoho tsa borena, ho ba le matla a masene a ho phekola.Re labalabela ho theha mekhatlo e metle le ea nako e telele ea mokhatlo hammoho le uena.
Professional Factory for China Amino Acid, Pharmaceutical, E sebelisa tsamaiso ea lefats'e e ka sehloohong bakeng sa ts'ebetso e ka tšeptjoang, tekanyo e tlaase ea ho hlōleha, e loketse khetho ea bareki ba Argentina.Khamphani ea rona e lutse ka har'a litoropo tsa naha tse tsoetseng pele, sephethephethe se bonolo haholo, maemo a ikhethang a sebaka le moruo.Re hahamalla tlhahiso e shebaneng le batho, ka bokhabane, ho fana ka maikutlo, ho haha ​​​​filosofi ea khoebo e ntle haholo.Tsamaiso e thata ea boleng, tšebeletso e phethahetseng, theko e loketseng Argentina ke boemo ba rona molemong oa tlholisano.Haeba ho hlokahala, amohela ho ikopanya le rona ka sebaka sa rona sa marang-rang kapa therisano ea mohala, re tla thabela ho u sebeletsa.