Karolelano e tlase ea A260/A230 hangata e bakoa ke litšila tse nang le maqhubu a phahameng a ho monya ho 230nm.Ha re bone hore na litšila tsena li kenyelletsa eng:

• Lintho tse silafatsang tse tloaelehileng	Leqhubu la ho monya	Karolelano ea phello
  Protheine	~ 230nm le 280nm	Ho fokotseha ha A260/A 280le A260/A 280likarolelano
  Letsoai la Guanidine	220-240 nm	Ho fokotsa palo ea A260/A 280karolelano
  Phenol	~ 270nm	-
  Trizol	~ 230nm le 270nm	Ho fokotsa palo ea A260/A 280karolelano
  EDTA	~230nm	Ho fokotsa palo ea A260/A 280karolelano
  Ethanol	230-240 nm	Ho fokotsa palo ea A260/A 280karolelano

 
 
 
Monyetla oa wavelength le boleng ba phapang ea litšila tse tloaelehileng

Ptšilafalo ea rotein
Tšilafalo ea protheine e ka nkuoa e le tšilafalo e atileng haholo ts'ebetsong ea ho ntša nucleic acid.Protheine e teng pakeng tsa karolo e ka holimo ea metsi le e ka tlaasemanyolomohato .Tšilafalo e tla fokotsa karo-karolelano ea A260/A280 le A260/A230 ka nako e le ’ngoe, ’me karo-karolelano ea A260/A230 e tla fetoha ka ho hlaka ho feta tekanyo ea A260/A280.
Nakong e tlangphetisetso e kgutlangor karabelo ea qPCR, masala a protheine a ka thibela kapa a sitisa tšebetso ea enzyme.Tsela e molemohali ea ho qoba tšilafalo ea protheine ke ho hopola molao-motheo oa "ho e-na le ho feta, palo e nyenyane ka makhetlo a mangata" ha u lakatsa matla a phahametseng tlhaho.

2. Tšilafalo ea Guanidinium
hydrochloride (GuHCl) le guanidine thiocyanate (GTC) li na le phello ea liprotheine tsa denaturing, tse ka senyang lisele tsa lisele ka potlako nakong ea ts'ebetso ea nucleic acid, e bakang protheine denaturation le precipitation.Bophahamo ba maqhubu a ho monya ha GuHCl le GTC bo pakeng tsa 220-240 nm, leletsoai la guanidinium le setseng le tla fokotsa karolelano ea A260/A230.Leha letsoai le setseng la guanidinium le tla fokotsa karo-karolelano,tšusumetso ea liteko tse tlase ha e le hantle ha e na thuso.

3. Tšilafalo ea Trizol
Karolo e ka sehloohong ea Trizol ke phenol.Mosebetsi o ka sehloohong oa phenol ke ho lisa lisele le ho lokolla liprotheine le lintho tsa nucleic acid liseleng.Le hoja phenol e ka senya liprotheine ka katleho, e ke ke ea thibela ka ho feletseng tšebetso ea RNase.Ka hona, 8 -hydroxyquinoline, guanidine isothiocyanate, β-mercaptoethanol, joalo-joalo li kenngoa ho TRIzol ho thibela RNase e sa feleng le e sa tloaelehang.
Ha e ntša RNA ea cellular, Trizol e ka senya lisele ka potlako 'me ea thibela nuclease e lokolloa liseleng,' me Trizol e setseng e tla fokotsa haholo karolelano ea A260 / A230.
Mokhoa oa ho sebetsa: Ha ho etsoa centrifuging, ho tlameha ho hlokomeloa hore phenol e Trizol e qhibiliha habonolo karolong ea metsi tlas'a boemo ba 4 ° le mocheso oa kamore.

4. Masala a Ethanol
Ethanol e sebelisoa ts'ebetsong ea ho qetela ho fokotsa DNA ha e ntse e qhaqha li-ion tsa letsoai tse ka 'nang tsa tlamahanngoa le DNA.Bokgale ba ho monya bo hodimodimotlhōrō ea ho monya eaethanol e boetse e le 230-240 nm, e lenghape e tla fokotsa karo-karolelano ea A260/A230.
Mokhoa oa ho qoba masala a ethanol o ka phetoa habeli nakong ea elution ea ho qetela, ho foka ka har'amosi oa mosimetsotso e 'meli ho lumella ethanol hore e fetohe mouoane ka ho feletseng pele o eketsa buffer bakeng sa elution.
Leha ho le joalo, ho lokela ho tsejoa hore karo-karolelano ke index ea tlhahlobo ea boleng ba RNA feela.Haeba ts'ebetso e boletsoeng ka holimo e laoloa ka thata, ho kheloha lipakeng tsa karolelano le mefuta e tloaelehileng e ke ke ea ba le tšusumetso e kholo litekong tse tlase.
Lihlahisoa tse Amanang:
Animal Total RNA Isolation kit
Semela Total RNA Isolation kit
Cell Total RNA Isolation kit
Plant Total RNA Isolation kit Plus