SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)
Tlhaloso ea Kit:
◮ PCR e tobileng:Ha ho na tlhoko ea "nucleic acid extaction kit" le "System" e phethiloeng ka mohlala oa 96 ka hora e le 'ngoe kapa ho feta.
◮ Tlhokahalo e tlase ea lisebelisoa le tšebeliso e feto-fetohang haholo:E hloka feela sistimi ea 'nete ea nako ea PCR.
◮ LoD le kutlo e phahameng:Boikutlo ba ho lemoha bo ka fihla boemong bo tlaase ka ho fetisisa joaloka likopi tse 500 / ml.
Tšebeliso e reriloeng
Molao-motheo oa teko
Sets'oants'o sena sa li-primer le probe se etselitsoe ho lemoha tatellano e bolokiloeng ea SARS-CoV-2 (ORF1ab gene, N gene le E gene).Thepa e na le Taolo ea ka Hare e sebelisetsoang ho lekola boleng ba mohlala.Theknoloji ea nako ea 'nete ea PCR e sebelisa polymerase chain reaction (PCR) bakeng sa ho holisa tatellano e khethehileng ea sepheo le li-probes tse tobileng bakeng sa ho lemoha RNA e matlafalitsoeng.Li-probe li ngotsoe ka moqolotsi oa litaba oa fluorescent le lidae tsa quencher.
Lintlha tse Potlakileng
◮Litlhaloso:48 Rxns/kit, 96 Rxns/kit
◮ Tsebo:96.72%
Kutloisiso:97.92%
Likaroloana
| Che. | Karolo | Chelete | Motsoako o ka sehloohong | |||
| 48 rxn | 96 Rxns | |||||
| 1 | Ho lokolloa ha Nucleic acidmoemeli | 1.5 mL / tube | 1 tube | 1.5 mL / tube | 1 tube | Surfactant |
| 2 | Tšireletso ea RNA | 27 μL / tube | 1 tube | 53 μL / tube | 1 tube | RNase inhibitor |
| 3 | SARS-CoV-2tharollo ea karabelo | 800 μL / tube | 1 tube | 1600 μL / tube | 1 tube | Primer, probe, buffer reaction, dNTP |
| 4 | SARS-CoV-2motsoako oa enzyme | 80 μL / tube | 1 tube | 160 μL / tube | 1 tube | Ho qala enzyme ea Taq,Enzyme ea M-MLV |
| 5 | SARS-CoV-2taolo e ntle | 100 μL / tube | 1 tube | 100 μL / tube | 1 tube | Recombinant plasmid e nang le sepheosekhechana, RNA |
| 6 | SARS-CoV-2taolo e mpe | 1200 μL / tube | 1 tube | 1200 μL / tube | 1 tube | TE buffer |
Likarolo le melemo
Mokhoa oa ho sebelisa
Mokhoa oa 1: Mokhoa o tobileng oa PCR
Mokhoa oa 2: Viral RNA Isolation + PCR
Mokhoa oa 1: Mokhoa oa PCR o tobileng o sebelisaMoemeli oa Phallo ea Mohlala
Mokhoa oa ho tseba
Lipalangoang le Polokelo ea Cold Chain
E tiisitsoe khanyeng 'me e bolokiloe ho -20±5℃;
Shelf bophelo
1 selemo
Ho Odara Boitsebiso
