1kb DNA Lere
Tlhaloso ea Kit:
◮1Kb DNA Ladder e entsoe ka likhoele tse 13 tse habeli tsa DNA, 'me khatello e habeli ea 1kb le 5kb e thusa ho khetholla lihlopha tse fapaneng.Ka tlase ho 1kb, lihlopha tse tharo tsa 250bp, 500bp, le 750bp lia eketsoa, 'me boholo ba sekhechana se sekhutšoane bo ka baloa ho latela sena.Khatiso ea lihlopha tse 'nè tsa 6kb, 7kb, 8kb, le 10kb e fokotsehile, e leng molemo ho fumana phello e ntle ea karohano.
Litlhaloso
1Kb DNA Ladder e entsoe ka likhoele tse 13 tse habeli tsa DNA, 'me khatello e habeli ea 1kb le 5kb e thusa ho khetholla lihlopha tse fapaneng.Ka tlase ho 1kb, lihlopha tse tharo tsa 250bp, 500bp, le 750bp lia eketsoa, 'me boholo ba sekhechana se sekhutšoane bo ka baloa ho latela sena.Khatiso ea lihlopha tse 'nè tsa 6kb, 7kb, 8kb, le 10kb e fokotsehile, e leng molemo ho fumana phello e ntle ea karohano.
Sehlahisoa sena se se se na le 1× Loading Buffer.U ka nka 2-6μl bakeng sa electrophoresis e tobileng ho latela litlhoko tsa teko.Setšoantšo sa electrophoresis se hlakile, se loketse ebile se sebetsa.
Lihlopha tse 13 tsa sehlahisoa sena ho tloha ho tse nyenyane ho ea ho tse kholo ke: 250bp, 500bp, 750bp, 1kb, 1.5kb, 2kb, 3kb, 4kb, 5kb, 6kb, 7kb, 8kb, 10kb.Haeba bophahamo ba modumo e le 5μl, 1kb le 5kb lihlopha ke 100ng;6kb, 7kb, 8kb, le 10kb lihlopha ke 25ng;'me lihlopha tse setseng ke 50ng.
Litlhaloso
| DL-30011 | 50 Preps (250μl) |
| DL-30012 | 200 Preps (1ml) |
Likarolo tsa Kit
Boitsebiso ba mokelikeli oa polokelo
10mM Tris-HCl(pH 8.4) 10mM EDTA
0.02% BPB
5% ea glycerin
Sehlahisoa sa mahloriso
Motsoako oa sehlahisoa o ka ba 130μg/ml
Electropherogram
Maemo a polokelo
Ka kopo, boloka ho 4℃(ka kopo boloka ho -20℃bakeng sa polokelo ea nako e telele)
