RT-PCR Easyᵀᴹ I(Mohato o le Mong)
Tlhaloso ea Kit:
◮Sesebelisoa sa mohato o le mong se etsa hore ho fetoleloa ka morao le PCR ho etsoe ka tube e le 'ngoe.E hloka feela ho eketsa template ea RNA, li-primer tsa PCR le RNase-Free ddH2O.
◮Tlhahlobo ea nako ea 'nete ea RNA e ka etsoa kapele le ka nepo.
◮Setsi sena se sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
◮Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
Litlhaloso
Foregene Mohato o le Mong RT-PCR BonoloTM lihlahisoa tsa letoto li lemoha karabelo e kopaneng ho tloha ho RNA ho isa ho DNA e nang le likhoele tse peli, ke hore, transcript le PCR amplification li phetheloa ka mokhoa o ts'oanang oa karabelo ea centrifuge tube le sistimi e ts'oanang ea karabelo, e nolofatsang mehato ea liteko., ntlafatsato ile a teko lenaneo, lentlafala teko bokgabane.
RT-PCR e bonoloTM I(Mohato o le Mong) ke mohato o le mong oa RT-PCR kit e entsoeng ka ho khetheha ke Foregene, e hlokomelang ts'ebetso e tsoelang pele ho tloha RT ho ea ho PCR ka tube e tšoanang.Ts'ebetso e bonolo ebile e potlakile, e ka fokotsang tšilafalo ka katleho nakong ea teko.2×RT-PCR e bonoloTM Motsoako o na le mamello e matla, botsitso ba mocheso, ts'ebetso e phahameng le e tobilengity bakeng sa matlafatso.
Likarolo tsa Kit
| RT-PCR e bonoloTMKe (Mohato o le mong) | ||
| Likahare tsa Kit | RT-02011 | RT-02012 |
| 100T (20μl sistimi) | 500T (20μl sistimi) | |
| 2 × RT-PCR e BonoloTM Kopanya | 1ml | 1.7ml×3 |
| RNase-Free ddH2O | 1.7ml | 1.7ml×3 |
| Buka ea Tataiso | 1 sengoathoana | 1 sengoathoana |
Likarolo le melemo
- Thepa ea mohato o le mong e thusa hore ho ngolisoa ka morao le PCR ho etsoe ka tube e le 'ngoe.E hloka feela ho eketsa template ea RNA, li-primer tsa PCR le RNase-Free ddH2O.
- Tlhahlobo ea nako ea sebele ea RNA e ka etsoa kapele le ka nepo.
- Thepa e sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
- Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
1.Maemo a lipalangoang
Ts'ebetso eohle ea lipalangoang tse tlase tsa mocheso o tlase oa leqhoa, ho etsa bonnete ba hore khiti e boemong ba <4 °C.
2. Maemo a polokelo
Thepa e bolokiloe ka mocheso oa -20 ° C.Boloka sehlahisoa ka sehatsetsing se tsitsitseng sa mocheso ho -20 ° C hang ka mor'a ho fumana.Haeba maemo a polokelo a loketse,tSehlahisoa sa hae se ke ke sa nyenyefatsa ts'ebetso efe kapa efe nakong ea selemo sa 1 sa netefatso.
