PCR Purification Kit PCR Clean-up System Bakeng sa Ultra-pure DNA
Tlhaloso ea Kit:
Ka potlako hloekisa le ho fumana likhechana tsa DNA tsa boleng bo holimo ho tsoa ho sistimi ea PCR;haeba u batla ho arola le ho fumana likhechana tse khethehileng tsa DNA, ka kopo khetha lisebelisoa tsa ho khutlisa li-gel.
◮ Mefuta e mengata ea ho hlaphoheloa ha DNA: Likaroloana tsa DNA tse khutšoane joalo ka 60bp le tse kholo joalo ka 10kb li ka fumanoa.
◮ Ts'ebetso e phahameng ea ho hlaphoheloa: Ts'ebetso ea ho hlaphoheloa hangata e feta 80%, 'me e phahameng ka ho fetisisa e ka fihla ho feta 95%.
◮ Lebelo le potlakileng:Ho bonolo ho sebetsa, ho hlaphoheloa ha sekhechana sa DNA ho ka phethoa nakong ea metsotso e 15 mme primer dimer e ka tlosoa ka kotloloho ntle le ho hlaphoheloa ha gel.
◮ Safety: Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮ Boleng bo phahameng: Likaroloana tse fumanoeng tsa DNA li hloekile haholo, tse ka kopanang le liteko tse fapaneng tse latelang.
Tlhaloso
Setsi sena se sebelisa "spin column le formula" e ntlafalitsoeng ke k'hamphani ea rona, e ka khutlisetsang likhechana tsa DNA tse hloekileng haholo tsamaisong ea PCR.Kholomo ea tlhoekiso e ka tlama DNA ka nepo, 'me e tsamaellana le reagent e ikhethang ea ho tlosa primer dimer ntle le ho hlaphoheloa ha gel.
Kit e fumana likarolo tse ngata tsa DNA.Tlas'a maemo a tloaelehileng, likhechana tsa DNA tse khutšoanyane joalo ka 60bp li ka fumanoa.Bonyane 30μl ea leseli e ka sebelisoa ho eketsa bongata ba DNA e fumanoeng.
Sesebelisoa se bonolo ho sebetsa mme se na le mehato e fokolang.E hloka feela centrifugation e 'maloa ho sebetsana le lisampole tse ngata ka nako e le' ngoe, 'me likaroloana tsa DNA tse fumanoeng ka bohloeki bo phahameng li ka fumanoa ka metsotso e 15.
Litlhaloso
50 Preps, 100Preps, 250Preps
Likarolo tsa Kit
| Buffer BD* |
| Buffer BD-S |
| Sekhahla sa WB1 |
| Buffer EB |
| DNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
- Mefuta e mengata ea ho hlaphoheloa ha DNA: Likaroloana tsa DNA tse khutšoanyane joaloka 60bp le tse kholo joaloka 10kb li ka fumanoa.
-Tlhaloso e phahameng ea ho hlaphoheloa: Kakaretso ea ho hlaphoheloa e atisa ho feta 80%, 'me e phahameng ka ho fetisisa e ka fihla ho feta 95%.
-Lebelo le potlakileng: Ho bonolo ho sebetsa, ho hlaphoheloa ha sekhechana sa DNA ho ka phethoa nakong ea metsotso e 15 mme primer dimer e ka tlosoa ka kotloloho ntle le ho hlaphoheloa ha gel.
-Tshireletso: Ha ho hlokahale ho ntšoa ha reagent ea organic.
-Boleng bo phahameng: Likaroloana tse fumanoeng tsa DNA li hloekile haholo, tse ka kopanang le liteko tse fapaneng tse latelang.
Sesebelisoa sa Kit
Setha sena se loketse ho khutlisa likhechana tsa DNA (60bp-10kb) lihlahisoa tsa PCR.
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho 2-8°C.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.
