Animal miRNA Isolation Kit MicroRNA Extraction and Purification Kits for Animal
Tlhaloso ea Kit:
Ka potlako le ka bokhabane ntša likhechana tsa RNA tsa 20-200nt miRNA, siRNA, snRNA ho tsoa liseleng tse fapaneng tsa liphoofolo.
◆Ts'ebetso eohle e etsoa ka mocheso oa kamore (15-25°C), ntle le ho hlatsoa leqhoa le mocheso o tlase oa centrifugation.
◆Sete e felletseng ea RNase-Free, ha ho na lebaka la ho tšoenyeha ka ho senyeha ha RNA.
◆DNA-Cleaning Column e tlama DNA ka ho khetheha, e le hore sesebelisoa se ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
◆Kotulo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
◆Lebelo le potlakileng: Ts'ebetso e bonolo ebile e ka phethoa nakong ea metsotso e 30.
◆Tšireletseho: ha ho hlokahale ho ntšoa ha reagent ea organic.
◆Boleng bo phahameng: Likaroloana tsa RNA tse ntšitsoeng li hloekile haholo, ha li na protheine le litšila tse ling, 'me li ka fihlela litlhoko tsa ho theosa le noka.
◆Lisebelisoa tse fapaneng tsa liteko
Tlhaloso
Setsi sena se sebelisa "spin column le formula" e ntlafalitsoeng ke k'hamphani ea rona, e ka ntšang likotoana tse nyane tsa RNA tse kang 20-200nt miRNA, siRNA, snRNA ho tsoa liseleng tse fapaneng tsa liphoofolo.The kit e fana ka DNA-Cleaning Column, e ka arolang habonolo supernatant ho tloha lysate ea lisele le ho tlosa DNA le RNA e telele ho tloha sampuling, e bonolo le e bolokang nako;Kholomo ea RNA-feela e ka tlama RNA ka mokhoa o phethahetseng ka foromo e ikhethang, E khona ho sebetsana le lisampole tse ngata ka nako e le 'ngoe.Tsamaiso eohle ke RNase-Free, e le hore miRNA e hloekisitsoeng e se ke ea senyeha;Buffer miRW1, Buffer miRW2 buffer system, e le hore RNA e fumanoeng e se na protheine, DNA, ion, le tšilafalo ea motsoako oa organic.
Likarolo tsa Kit
| Animal miRNA Isolation Kit | ||||
| Likahare tsa Kit | RE-0101T | RE-01011 | RE-01012 | RE-01014 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miRL* | 3ml | 25ml | 50ml | 100ml |
| Buffer miRW1 | 4ml | 35ml | 70ml | 140ml |
| Buffer miRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-Free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| Lekholomo la RNA feela | 5 lihlopha | 50lihlopha | 100lihlopha | 200lihlopha |
| DNA-Ho hloekisa Column | 5 lihlopha | 50lihlopha | 100lihlopha | 200lihlopha |
| IFU | 1 sengoathoana | 1 sengoathoana | 1 sengoathoana | 1 sengoathoana |
Likarolo le melemo
◆Ts'ebetso eohle e etsoa ka mocheso oa kamore (15-25°C), ntle le ho hlatsoa leqhoa le mocheso o tlase oa centrifugation.
◆Sete e felletseng ea RNase-Free, ha ho na lebaka la ho tšoenyeha ka ho senyeha ha RNA.
◆DNA-Cleaning Column e tlama DNA ka ho khetheha, e le hore sesebelisoa se ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
◆Kotulo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
◆Lebelo le potlakileng: Ts'ebetso e bonolo ebile e ka phethoa nakong ea metsotso e 30.
◆Tšireletseho: ha ho hlokahale ho ntšoa ha reagent ea organic.
◆Boleng bo phahameng: Likaroloana tsa RNA tse ntšitsoeng li hloekile haholo, ha li na protheine le litšila tse ling, 'me li ka fihlela litlhoko tsa ho theosa le noka.
◆Lisebelisoa tse fapaneng tsa liteko
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha miRNA ho tsoa ho mefuta e fapaneng ea lisele tse foreshe kapa tse leqhoa.
Storage le Shelf bophelo
Setsi sena se ka bolokoa likhoeli tse 24 tlas'a maemo a omileng mocheso oa kamore (15-25°C), haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho2-8°C.Buffer miRL e ka behoa ho4°Cbakeng sa 1 khoeli ka mor'a ho eketsaβ-mercaptoethanol(ho khothaletsoa ho e eketsa hona joale).
