Blood SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Blood Direct PCR Master Mix bakeng sa Genotyping of Blood
Tlhaloso ea Kit:
Sebelisa ka kotloloho mali a felletseng a anticoagulated ea heparin joalo ka template ea ho holisa PCR, e nang le kutlo e phahameng le bokhoni bo phahameng ba ho holisa.Lenaneo la UNG le khahlanong le tšilafalo le ekelitsoe ho thibela mekhoa e fosahetseng.
◮Sistimi ena e na le matla a ho holisa 'me e khona ho bona ka hloko liphatsa tsa lefutso tsa DNA le likhechana tsa DNA maling.
◮2 × SuperEasyTMMix(UNG) -Heparin Mamello e phahameng ea mali: ho fihla ho 45% maling a motho le 20% maling a toeba.
◮Mohlala o koaletsoe ka botlalo, kahoo ha ho na lebaka la ho tšoenyeha ka tšilafalo ea sampole le liphetho tse fosahetseng tsa PCR.Sistimi e khahlanong le tšilafalo ea PCR
◮2 × SuperEasyTMKopanya (UNG), ka katleho felisa tšilafalo e bakoang ke lihlahisoa tsa PCR, 'me u netefatse ho nepahala le ho nepahala ha amplification.
Litlhaloso
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMMix (UNG) -Heparin: Motheong oa 2 × SuperEasyTMMix-Heparin, dUTP e sebelisoa ho e-na le dTTP, 'me enzyme ea UNG e ka senyang template ea dUTP e kenngoa ka nako e le' ngoe, e ka thibelang ka katleho ho silafala ha lihlahisoa tsa amplification tsa PCR.E na le D-Taq DNA Polymerase e fetotsoeng, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer le stabilizer, joalo-joalo. Boemong ba PCR, kenya feela mali, li-primers le ddH tse nepahetseng.2O ho 2 × SuperEasyTM Mix (UNG) -Heparin e lumellanang le eona e lokelang ho sebelisoa karabelo ea PCR.
6 × DNA Loading Buffer: The Loading Buffer ha e na SDS.Ho khothalletsoa ho sebelisa 6 × DNA Loading Buffer e kenyellelitsoeng le kit ha u etsa agarose gel electrophoresis e le hore u fumane liphello tse ntle tsa electrophoresis.U se ke ua sebelisa Loading Buffer e nang le SDS, ho seng joalo ho tla ba le mohatla o moholo oa khanya e khanyang tseleng nakong ea electrophoresis, e tla ama liphello tsa liteko.
Likarolo tsa Kit
| 2 × SuperEasyTMMotsoako(UNG)-Heparin |
| 6× DNA Loading Buffer |
| Litaelo |
Likarolo le melemo
-Sistimi ena e na le matla a ho holisa 'me e khona ho bona ka hloko liphatsa tsa lefutso tsa DNA le likhechana tsa DNA maling.
-2 × SuperEasyTMMix(UNG) -Heparin Mamello e phahameng ea mali: ho fihla ho 45% maling a motho le 20% maling a toeba.
-Mohlala o koaletsoe ka botlalo, kahoo ha ho na lebaka la ho tšoenyeha ka tšilafalo ea sampole le liphetho tse fosahetseng tsa PCR.Anti-tšilafalo tsamaiso ea PCR 2 × SuperEasyTMKopanya (UNG), ka katleho felisa tšilafalo e bakoang ke lihlahisoa tsa PCR, 'me u netefatse ho nepahala le ho nepahala ha amplification.
Sesebelisoa sa Kit
E nehetsoe ho tsebahatsa PCR e tobileng ea mali a Heparin a anticoagulated.(Ho kenyeletsoa: mali a motho, mali a toeba, mali a khoho, mali a nonyana, mali a khomo, mali a ntja, joalo-joalo)
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
Ts'ebetso eohle ea lipalangoang tsa li-ice box tsa mocheso o tlase, ho netefatsa hore kit e boemong ba <4 ℃.
2 × SuperEasyTMMix(UNG) -Heparin, haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4 ° C bakeng sa polokelo ea nako e khutšoanyane (sebelisa pele ho matsatsi a 10).
6× DNA Loading Buffer, e ka bolokoa ho 4°C kapa -20°C nako e telele.
