Blood SuperDirectᵀᴹ PCR Kit-EDTA Blood Direct PCR Master Mix bakeng sa Genotyping of Blood
Tlhaloso ea Kit:
Sebelisa ka kotloloho mali a EDTA a anticoagulated e le template ea ho holisa PCR, e nang le kutlo e phahameng le matla a ho holisa.
◮Ha ho na tlhoko ea tlhoekiso ea DNA e jang nako e ngata le e theko e boima, ha ho na kalafo, le tšebeliso e tobileng ea mali a felletseng joalo ka template ea PCR.
◮Sistimi ena e na le matla a ho holisa 'me e khona ho bona ka hloko liphatsa tsa lefutso tsa DNA le likhechana tsa DNA maling.
◮2 × SuperEasyTMMix-EDTA Mamello e phahameng ea mali: ho fihla ho 45% maling a motho le 20% maling a toeba.
◮Mohlala o koaletsoe ka botlalo, kahoo ha ho na lebaka la ho tšoenyeha ka tšilafalo ea sampole le liphetho tse fosahetseng tsa PCR.
Litlhaloso
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMMix-EDTA: E na le D-Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer le stabilizer, joalo-joalo2O ho 2 × SuperEasy e lumellanangTMMix-EDTA e tla sebelisoa karabelong ea PCR.
6 × DNA Loading Buffer: The Loading Buffer ha e na SDS.Ho khothalletsoa ho sebelisa 6 × DNA Loading Buffer e kenyellelitsoeng le kit ha u etsa agarose gel electrophoresis e le hore u fumane liphello tse ntle tsa electrophoresis.U se ke ua sebelisa Loading Buffer e nang le SDS, ho seng joalo ho tla ba le mohatla o moholo oa khanya e khanyang tseleng nakong ea electrophoresis, e tla ama liphello tsa liteko.
Likarolo tsa Kit
| 2 × SuperEasyTMKopanya-EDTA |
| 6× DNA Loading Buffer |
| Litaelo |
Likarolo le melemo
-Ha ho na tlhokahalo ea ho hloekisa DNA e jang nako le e theko e boima, ha ho na phekolo ea pele, le tšebeliso e tobileng ea mali a feletseng e le template ea PCR.
-Sistimi e na le matla a matla a ho holisa 'me e khona ho lemoha ka hloko liphatsa tsa lefutso tsa DNA le liphatlalatso tsa kantle ho naha maling.
-2 × SuperEasyTMMix-EDTA Mamello e phahameng ea mali: ho fihla ho 45% maling a motho le 20% maling a toeba.
-Mohlala o koaletsoe ka botlalo, kahoo ha ho na lebaka la ho tšoenyeha ka tšilafalo ea sampole le liphetho tse fosahetseng tsa PCR.
Sesebelisoa sa Kit
E nehetsoe ho tsebahatsa PCR e tobileng ea mali a EDTA a anticoagulated.(Ho kenyeletsoa: mali a motho, mali a toeba, mali a khoho, mali a nonyana, mali a khomo, mali a ntja, joalo-joalo)
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
Sekhahla sena se lokela ho bolokoa ka mocheso oa -20 ° C.
2 × SuperEasyTMMix-EDTA, haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4 ℃ bakeng sa polokelo ea nako e khutšoanyane (sebelisa pele ho matsatsi a 10).
6× DNA Loading Buffer, e ka bolokoa ho 4°C kapa -20°C nako e telele.
