Baktheria DNA Isolation Kit Bacterial Genomic DNA Extraction Purification Kits
Tlhaloso ea Kit:
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa ho libaktheria karolong ea kholo ea logarithmic.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
Tlhaloso
Setsi sena se fana ka mokhoa o potlakileng le o bonolo oa ho ntša DNA ea genomic ho litloaelo tsa libaktheria (Gram-negative le Gram-positive) mehloling e sa tšoaneng;e ka sebetsa ka tlase ho 3ml ea litloaelo tsa baktheria karolong ea kholo ea logarithmic (libaktheria tsa 1 × 109).Thepa e tsamaisana le Foregene Protease e sebetsang hantle haholo, e lumellang 15-50μg ea DNA ea boleng bo holimo hore e ntšoe nakong ea hora e le 'ngoe.Ntle le moo, kit e ka boela ea ntša liphatsa tsa lefutso ntle le genome, joalo ka plasmids, Cosmid, BAC, jj.
Thepa ea matrix ea silica gel ea DNA feela e sebelisitsoeng mokokotlong oa spin ke thepa e ncha e ikhethang ea k'hamphani, e ka bapang DNA ka nepo le ka ho khetheha.Bokhoni bo boholo ba ho bapatsa bakeng sa DNA ke 80μg.Sistimi e ikhethang ea buffer le elution e ka eketsa ho tlosoa ha RNA, liprotheine tse sa hloekang, li-ion le metsoako e meng ea tlhaho ka liseleng.Sekhechana sa DNA se nkiloeng se seholo, se hloekile haholo, se tsitsitse ebile ke boleng bo ka tšeptjoang, 'me boholo ba sekhechana sa DNA bo tsitsitse hoo e ka bang 23kb.
Litlhaloso
50 Preps, 100 Preps, 250 Preps
Likarolo tsa Kit
| Buffer ML1 |
| Buffer ML2 |
| Sekhahla sa PW |
| Buffer WB |
| Buffer EB |
| Foregene Protease |
| Lysozyme |
| Buffer TE |
| DNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
-Ha ho tšilafalo ea RNase: Ha ho na tlhoko ea ho eketsa RNase ho tlosa RNA ho DNA ea genomic, ho qoba laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
-Lebelo le potlakileng: Foregene Protease e na le ts'ebetso e phahameng ho feta ea protease e tšoanang 'me e khona ho sileha lisampole tse robehileng tsa baktheria ka nako e khuts'oane.
-Bonolo: The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale hore 4 ° C mocheso o tlaase oa centrifugation kapa ethanol precipitation ea DNA.
-Tshireletso: ha ho hlokahale ho ntšoa ha reagent ea organic.
-Boleng bo phahameng: Likaroloana tse nkiloeng tsa genomic DNA li kholo, ha ho na RNA, ha ho RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse fapa-fapaneng.
Sesebelisoa sa Kit
Setsi sena se loketse ho hloekisa DNA ea genomic ea lisampole tse latelang: Libaktheria tsa gram-negative le baktheria ea Gram-positive mokhahlelong oa kholo ea logarithmic.
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
-Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho 2-8°C.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o kenya tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.
-Forene Protease tharollo e na le mokhoa o ikhethang, o sebetsang ha o bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);ha e bolokiloe ka 4 ° C, mosebetsi oa eona le botsitso li tla ba molemo, kahoo ho kgothaletswa ho e boloka ho 4 ° C, hopola hore u se ke ua e boloka ho -20 ° C.
-Lysozyme ke phofo e omeletseng, e sebetsang ha e bolokiloe ho 2-8 ° C ka nako e telele (likhoeli tse 3).Bakeng sa polokelo e telele, ka kopo e boloke ho -20°C.
