RT-qPCR Easyᵀᴹ (Mohato o le Mong)-Taqman
Tlhaloso ea Kit:
- E 'ngoe-mohato oa mohato o nolofalletsa ho ngoloa ka morao le PCR ho etsoa ka tube e le 'ngoe, ho hlokahala feela ho eketsa template ea RNA, li-primers tse khethehileng tsa PCR le RNase-Free ddH.2O.
- Tlhahlobo ea nako ea 'nete ea palo ea RNA ea vaerase kapa trace RNA e ka etsoa kapele le ka nepo.
- Thepa e sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
- Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
- RT-qPCR e bonoloTM(Mohato o le mong) -Taqman kit e tla le dae ea ka hare ea ROX, e ka sebelisoang ho tlosa semelo sa matšoao le liphoso tsa matšoao lipakeng tsa liliba, tse loketseng basebelisi ba ho qetela ho li sebelisa mefuteng e fapaneng ea lisebelisoa tsa PCR tse ngata.
Litlhaloso
Lihlahisoa tsa Foregene One-Mohato oa RT-PCR Easy li hlokomela karabelo e kopaneng ho tloha ho RNA ho isa ho DNA e nang le likarolo tse peli, ke hore, phetisetso ea morao-rao le ntlafatso ea PCR e phetheloa ka mokhoa o ts'oanang oa karabelo ea centrifuge le sistimi e ts'oanang ea karabelo, ka hona mehato ea liteko e nolofalitsoe, leano la liteko le ntlafalitsoe, le katleho ea liteko ea ntlafatsoa.
RT-qPCR e bonoloTM (E mongMohato)-Taqmane sebelisa Foregene HotStar Taq DNA Polymerase, sistimi e ntlafalitsoeng ea Taqman qPCR e ka khona ho etsa tlhahlobo ea nako ea 'nete ea RT-qPCR ea trace ea litempele tsa RNA kapele le ka ho khetheha.
Litlhaloso
100 Rxns (20μl system)
500 Rxns (20μl system)
Likarolo tsa Kit
| RT-qPCR EasyTM (Mohato o le Mong) -Taqman | ||
| Likarolo tsa Kit | RT-02131 | RT-02132 |
| 100Rxns (20μl sistimi) | 500 Rxns (20μl system) | |
| 2 × RT-qPCR e BonoloTMKopanya-Taqman | 1ml | 1.7ml×3 |
| 20 × ROX Reference Dye | 100μl | 0.5ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml×3 |
| Taelo Bakeng sa ho Sebelisa | 1 | 1 |
Likarolo le melemo
- Kit ea mohato o le mong e thusa ho fetolela ka morao le PCR hore e etsoe ka har'a tube e le 'ngoe, e hloka feela ho eketsa template ea RNA, li-primer tsa PCR le RNase-Free ddH.2O.
- Tlhahlobo ea nako ea 'nete ea palo ea RNA ea vaerase kapa trace RNA e ka etsoa kapele le ka nepo.
- Thepa e sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
- Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
- RT-qPCR e bonoloTM(Mohato o le mong) -Taqman kit e tla le dae ea ka hare ea ROX, e ka sebelisoang ho tlosa semelo sa matšoao le liphoso tsa matšoao lipakeng tsa liliba, tse loketseng basebelisi ba ho qetela ho li sebelisa mefuteng e fapaneng ea lisebelisoa tsa PCR tse ngata.
Litekanyetso tsa kit
Sesebelisoa sa Kit
- Setsi sena se sebelisetsoa ho lemoha kapele le ka nepo li-RNA tsa trace joalo ka livaerase tsa RNA.
- E loketse ka ho khetheha bakeng sa ho lemoha ka potlako litempele tsa RNA tse nang le litaba tse phahameng tsa GC kapa sebopeho se rarahaneng sa bobeli.
Mokhoa oa ho sebetsa
Maemo a polokelo
1.Lipalangoangmaemo
Ts'ebetso eohle ea lipalangoang tsa li-ice box tsa mocheso o tlase, ho netefatsa hore kit e boemong ba <4 ℃.
2.Polokelo maemo
Thepa e bolokiloe ho -20 ℃.Boloka sehlahisoa ka sehatsetsing sa mocheso sa kamehla ho-20 ℃ hang ka mor'a ho fumana.Haeba maemo a polokelo a loketse, sehlahisoae ke ke ea nyenyefatsa ts'ebetso efe kapa efe nakong ea selemo sa 1 sa ho sebetsa.
