RT-PCR Easyᵀᴹ II(Mehato e Meli)
Tlhaloso ea Kit:
◮Tlhahlobo ea nako ea 'nete ea RNA e ka etsoa kapele le ka nepo.
◮Setsi sena se sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
◮ Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
Litlhaloso
RT-PCR e bonoloTM II(Mehato e 'meli) e na le li-reagents tsohle tsa mehato e 'meli ea RT-PCR, e kopanyang karabelo ea RT le karabelo ea PCR ka har'a kit e le 'ngoe, e fana ka RT Mix e ntlafalitsoeng le PCR Mix.Motsoako o ikhethileng oa RT Mix o ka kopanya cDNA ea boleng bo holimo habonolo le ka mokhoa o atlehileng. 2× PCRBonoloTM KopanyaHape e na le matla a holimo le matlafatso e khethehileng.Motsoako oa lintho tse peli tse phelang o etsa hore ho sibolloa ha jini e reretsoeng ho be bonolo haholoanyane.
Likarolo tsa Kit
| RT-PCRBonoloTM II(Mehato e 'meli) | ||
| Likahare tsa Kit | RT-02021 | RT-02022 |
| 100T(20μltsamaiso) | 500T(20μltsamaiso) | |
| 2 × RT E bonoloTM Kopanya | 1ml | 1.7ml×3 |
| 2× PCRBonoloTM KopanyaHape | 1ml | 1.7ml×3 |
| Primer e sa fetoheng (50μM) | 200μl | 1ml |
| Oligo(dT)18 Primer (50μM) | 200μl | 1ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml |
| Buka ea Tataiso | 1sengoathoana | 1sengoathoana |
Likarolo le melemo
-Tlhahlobo ea nako ea 'nete ea RNA e ka etsoa kapele le ka nepo.
-Setsi sena se sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
-Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
1.Maemo a lipalangoang
Ts'ebetso eohle ea lipalangoang tse tlase tsa mocheso o tlase oa leqhoa, ho etsa bonnete ba hore khiti e boemong ba <4 °C.
2. Maemo a polokelo
Thepa e bolokiloe ka mocheso oa -20 ° C.Boloka sehlahisoa ka sehatsetsing se tsitsitseng sa mocheso ho -20 ° C hang ka mor'a ho fumana.Haeba maemo a polokelo a loketse,tSehlahisoa sa hae se ke ke sa nyenyefatsa ts'ebetso efe kapa efe nakong ea selemo sa 1 sa netefatso.
