Mali a DNA Mini Kit
Tlhaloso ea Kit:
Ka potlako hloekisa DNA ea boleng bo phahameng ba genomic maling a anticoagulated (<1ml).
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
Litlhaloso tsa Kit
Setsi sena se nka Foregene Protease Plus e ncha le lisebelisoa tse ikhethang tsa BL1 le BL2, tse ka khonang ho cheka lisampole tsa mali tsa anticoagulant ka nako e khuts'oane, kahoo ho qoba ho senyeha ha DNA ho isa tekanyong e kholo le ho fumana boholo ba DNA ea genomic.Sistimi e potlakileng ea ts'ebetso ea mali le ts'ebetso e bonolo ea DNA-Feela e nolofatsa haholo ho ntšoa ha genome ea mali, ho etsa hore DNA ea genomic e ka fumanoa ka boleng bo holimo le bohloeki bo phahameng nakong ea metsotso e 40.
DNA-feela silica gel membrane e sebelisitsoeng ka har'a kholomo ea centrifuge ea kit ena ke mofuta o mocha oa thepa e ikhethang ho k'hamphani ea rona, e ka tlamang DNA ka katleho le ka ho khetheha, 'me e ka tlosa RNA, litšila, liprotheine, li-ion le metsoako e meng ea lintho tse phelang liseleng ho isa tekanyong e phahameng.Likaroloana tse fumanoeng tsa DNA li kholo, bohloeki bo phahameng, bo tsitsitse ebile bo tšepahala.Bophahamo bo boholo ba kholomo ea DNA-Feela ke 80 μg DNA.DNA e fumanoeng e ka sebelisoa litekong tsa baeloji ea limolek'hule tse kang tšilo ea enzyme, PCR, hybridization ea Boroa le kaho ea laeborari.
Thepa e ka sebetsa ho fihlela ho 1ml ea mali ka nako ho fumana palo e lekaneng ea DNA ea genomic e hloekileng.
Litlhaloso
| Mali a DNA Mini Kit | |||
| Likahare tsa Kit | DE-05111 | DE-05112 | DE-05113 |
| 50 T | 100 T | 250 T | |
| Buffer BL1 | 15 ml | 30 ml | 75 ml |
| Buffer BL2 * | 15 ml | 30 ml | 75 ml |
| Buffer DC | 100 ml | 200 ml | 500 ml |
| Buffer PW * | 25 ml | 50 ml | 125 ml |
| Sekhahla sa WB1 | 15 ml | 30 ml | 75 ml |
| Buffer EB | 10 ml | 20 ml | 50 ml |
| Foregene Protease Plus | 1.25 mL | 2.5 ml | 6.5 ml |
| DNA-Feela Kholomo | 50 | 100 | 250 |
| Bukana | 1 | 1 | 1 |
*: Buffer BL2 le Buffer PW e na le letsoai la metsi le hlabang.Ka kopo, roala liatlana 'me u nke mehato e nepahetseng ea ts'ireletso ha u sebetsa.
Likarolo le melemo
-Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho qoba laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
-Lebelo le potlakileng: Foregene Protease Plus e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso e 40.
-Bonolo: The centrifugation e etsoa mocheso oa kamore, 4 ° C centrifugation kapa ethanol precipitation ea DNA ha e hlokehe.
-Tshireletso: Ha ho qotsulo ea organic reagent e sebelisoang.
-Boleng bo phahameng: Likaroloana tse nkiloeng tsa genomic DNA li kholo, ha ho na RNA, ha ho RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse fapa-fapaneng.
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisa DNA ea genomic maling a foreshe kapa a leqhoa a anticoagulated.
Phallo ea mosebetsi
Polokelo le shelf bophelo
Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho 2-8°C.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.
Tharollo ea Foregene Protease Plus e na le foromo e ikhethang, e sebetsang ha e bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);e tla ba e sebetsang haholoanyane le e tsitsitseng ha e bolokiloe ho 4 ° C, kahoo ho kgothaletswa ho e boloka ho 4 ° C, hopola hore u se ke ua e boloka ho -20 ° C.
