Viral RNA Isolation Kit bakeng sa Tlhoekiso ea Viral RNA ho tsoa Plasma, Serum, le Mehlala e meng.
Tlhaloso ea Kit:
Katse.No.RE-02011/02014
Bakeng sa tlhoekiso ea RNA ea kokoana-hloko ho tsoa ho plasma, serum, maro a 'mele a se nang lisele, li-supernatant tsa setso sa lisele.
Ka potlako arola le ho hloekisa RNA ea kokoana-hloko ho lisampole tse kang plasma, serum, maro a 'mele a se nang lisele le li-supernatant tsa cell culture.
-Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.Thepa eohle ha e na RNase-Free
-E bonolo - ts'ebetso eohle e phethoa ka mocheso oa kamore
-Ka potlako-ts'ebetso e ka phethoa ka metsotso e 20
-Sehlahisoa se phahameng sa RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle
-Sireletsehile-ha ho na reagent ea tlhaho e sebelisoang
-Mehlala e meholo ea ho sebetsa - ho fihlela ho 200μl lisampole li ka sebetsoa nako le nako.
-Boleng bo phahameng-RNA e hloekisitsoeng e hloekile haholo, ha e na liprotheine le litšila tse ling, 'me e ka kopana le mekhoa e fapaneng ea liteko tse tlaase.
Litlhaloso
Thepa e sebelisa "spin column le formula" e ntlafalitsoeng ke Foregene, e ka ntšang RNA ea boleng bo holimo le ea boleng bo holimo ho tsoa lisampoleng tse kang plasma, serum, mokelikeli oa 'mele o se nang lisele le matla a matla a lisele.Kit e eketsa ka ho khetheha Linear Acrylamide, e ka nkang RNA e nyane habonolo ho tsoa lisampoleng.RNA-Feela Column e ka tlama RNA hantle.Kit e ka sebetsana le palo e kholo ea lisampole ka nako e le 'ngoe.
Thepa eohle ha e na RNase, kahoo RNA e hloekisitsoeng e ke ke ea senyeha.Buffer viRW1 le Buffer viRW2 e ka etsa bonnete ba hore varal nucleic acid e fumanoeng ha e na protheine, nuclease kapa litšila tse ling, e ka sebelisoang ka kotloloho bakeng sa liteko tsa baeloji ea limolek'hule.
Litlhaloso
50 Preps, 200 Preps
Likarolo tsa Kit
| Linear Acrylamide |
| Buffer viRL |
| Buffer viRW1, Buffer viRW2 |
| RNase-Free ddH2O |
| RNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
-Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.Thepa eohle ha e na RNase-Free
-E bonolo - ts'ebetso eohle e phethoa ka mocheso oa kamore
-Ka potlako-ts'ebetso e ka phethoa ka metsotso e 20
-Sehlahisoa se phahameng sa RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle
-Sireletsehile-ha ho na reagent ea tlhaho e sebelisoang
-Mehlala e meholo ea ho sebetsa - ho fihlela ho 200μl lisampole li ka sebetsoa nako le nako.
-Boleng bo phahameng-RNA e hloekisitsoeng e hloekile haholo, ha e na liprotheine le litšila tse ling, 'me e ka kopana le mekhoa e fapaneng ea liteko tse tlaase.
Litekanyetso tsa kit
Sesebelisoa sa Kit:
E loketse ho ntšoa le ho hloekisoa ha kokoana-hloko ea RNA ka lisampole tse kang plasma, serum, mokelikeli oa 'mele o se nang lisele le cell culture supernatant.
Phallo ea mosebetsi
Maemo a polokelo
- Thepa e ka bolokoa likhoeli tse 24 mocheso oa kamore (15-25 ℃), kapa 2-8 ℃ nako e telele.
-Linear Acrylamide tharollo e ka bolokoa ka mocheso oa kamore ka matsatsi a 7.Kamora ho amohela kit, ka kopo ntša tharollo ea Linear Acrylamide 'me u e boloke ho -20 ℃.
-Ka mor'a ho eketsa Linear Acrylamide ho Buffer viRL, e ka bolokoa ho 2-8 ℃ ho fihlela ho 48h.Ka kopo sebelisa tharollo e ncha e lokiselitsoeng.
Litaelo tsa tlhahlobo ea mathata
The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail：Tech@foregene.com。
Ha ho RNA e ka ntšoang kapa chai ea nucleic acid e tlase
Hangata ho na le lintlha tse ngata tse amang katleho ea ho hlaphoheloa, joalo ka: sampole ea litaba tsa RNA, mokhoa oa ts'ebetso, bophahamo ba molumo, joalo-joalo.
Tlhahlobo ea lisosa tse tloaelehileng:
1.Bate ea leqhoa kapa mocheso o tlase (4 ° C) centrifugation nakong ea ts'ebetso.
Tlhahiso: Mocheso oa kamore (15-25 ° C) o sebetsa, ha ho mohla ho hlatsoa leqhoa le mocheso o tlase oa centrifuge.
2. Polokelo e sa nepahalang ea mohlala kapa polokelo ea mohlala nako e telele haholo.
Tlhahiso: Boloka lisampole ho -80 ° C kapa u hatsele ka har'a metsi a naetrojene, 'me u qobe tšebeliso ea ho qhibiliha khafetsa;leka ho sebelisa lisampole tse sa tsoa bokelloa bakeng sa ho ntša RNA.
3.Sample e sa lekaneng ea lysis
Keletso: Ka kopo etsa bonnete ba hore sampole le tharollo e sebetsang (Linear Acrylamide) li tsoakoe ka botlalo 'me li kentsoe metsotso e 10 mocheso oa kamore (15-25 ° C)
4.The eluent e kentsoe ka phoso
Keletso: Etsa bonnete ba hore RNase-Free ddH2O e kentsoe bohareng ba lera la kholomo ea tlhoekiso.
5.Molumo o sa nepahalang oa ethanol ea anhydrous ho Buffer viRW2
Tlhahiso: Ka kopo latela litaelo, eketsa molumo o nepahetseng oa ethanol ea anhydrous ho Buffer viRW2 'me u li kopanye hantle pele u sebelisa kit.
6.Tsebeliso e fosahetseng ea sampole.
Tlhahiso: 200µl ea sampole ka 500μl ea Buffer viRL.Bophahamo bo feteletseng ba sampole bo tla fella ka ho fokotseha ha sekhahla sa ho ntšoa ha RNA.
7.Molumo o sa nepahalang oa tlhahiso kapa tlhahiso e sa fellang.
Tlhahiso: Molumo o hlakileng oa kholomo ea tlhoekiso ke 30-50μl;haeba phello ea elution e sa khotsofatse, ho kgothaletswa ho eketsa RNase-Free ddH e futhumetseng2O le ho eketsa nako ea ho beha mocheso oa kamore, joalo ka 5-10min
8.Kolumo ea tlhoekiso e na le masalla a ethanol ka mor'a ho hlatsoa Buffer viRW2.
Tlhahiso: Haeba ethanol e ntse e sala ka mor'a hore e hlatsoe ka Buffer viRW2 le centrifugation e se nang letho bakeng sa 2min, kholomo ea tlhoekiso e ka siuoa mocheso oa kamore bakeng sa 5min ka mor'a hore ho be le letho-tube centrifugation ho tlosa ethanol e setseng ka botlalo.
Ho senyeha ha limolek'hule tsa RNA tse hloekisitsoeng
Boleng ba RNA e hloekisitsoeng bo amana le lintlha tse kang polokelo ea sampuli, tšilafalo ea RNase, le ts'ebetso.
Tlhahlobo ea lisosa tse tloaelehileng:
1.Lisampole tse bokeletsoeng ha lia ka tsa bolokoa ka nako.
Tlhahiso: Haeba sampole e sa sebelisoe ka nako kamora ho bokelloa, ka kopo e boloke ho -80 ℃ kapa metsi a naetrojene hang-hang.Bakeng sa ho ntša limolek'hule tsa RNA, leka ho sebelisa lisampole tse sa tsoa bokelloa neng kapa neng ha ho khoneha.
2.Collected disampole ba ne ba hoama le ho qhibiliha khafetsa.
Tlhahiso: Qoba ho qhoqhoa khafetsa le ho qhibiliha (e seng ho feta hanngoe) nakong ea ho bokella le ho boloka sampole, ho seng joalo chai ea nucleic acid e tla fokotseha.
3.RNase e ile ea hlahisoa ka kamoreng ea ho buuoa kapa ho ne ho se na liatlana tse lahliloeng, limaske, joalo-joalo.
Tlhahiso: Ho ntšoa ha liteko tsa limolek'hule tsa RNA ho etsoa hantle ka phaposing e arohaneng ea tšebetso ea RNA, 'me tafole ea liteko e hloekisoa pele ho teko.Apara liatlana le limaske tse lahloang nakong ea liteko ho qoba ho senyeha ha RNA ho bakoang ke kenyelletso ea RNase.
4.The reagent e silafalitsoe ke RNase nakong ea tšebeliso.
Tlhahiso: Kenya sebaka ka Viral RNA Isolation Kit e ncha bakeng sa liteko tse amanang.
5. Tšilafalo ea RNase ea liphaephe tsa centrifuge, malebela a pipette, joalo-joalo. Tlhahiso: Etsa bonnete ba hore li-tubes tsa centrifuge, malebela a pipette, le lipeipi kaofela ha li na RNase.
Limolek'hule tsa RNA tse hloekisitsoeng li ile tsa ama liteko tse tlase
Limolek'hule tsa RNA tse hloekisitsoeng ke kholomo ea tlhoekiso li tla ama liteko tse tlase tsa metsi haeba ho na le li-ion tsa letsoai kapa liprotheine tse ngata haholo, joalo ka: reverse transcription, Northern Blot, joalo-joalo.
1.Ho na le li-ion tse setseng tsa letsoai ka har'a limolek'hule tsa eluted RNA.
Keletso: Etsa bonnete ba hore molumo o nepahetseng oa ethanol ea anhydrous e kentsoe Buffer viRW2, 'me u hlatsoe kholomo ea tlhoekiso habeli ho latela lebelo le nepahetseng la centrifugation litaelong tsa ts'ebetso; Haeba ho ntse ho e-na le li-ion tsa letsoai tse setseng, u ka eketsa Buffer viRW2 kholomong ea tlhoekiso, 'me u e tlohele mocheso oa kamore bakeng sa 5min.Ebe u etsa centrifugation ho tlosa silafatso ea ion ea letsoai ka tekanyo e kholo ka ho fetisisa
2.Ho na le ethanol e setseng ka har'a limolek'hule tsa RNA
Tlhahiso: hang ha o netefatsa hore litšiea tsa tlhoekiso li hlatsoitsoe ke Buffer viRW2, etsa centrifugation e se nang letho ho latela lebelo la centrifugal ho litaelo tsa ts'ebetso.Haeba ho ntse ho e-na le ethanol e setseng, e ka tloheloa metsotso e 5 mocheso oa kamore ka mor'a hore ho be le centrifugation e se nang letho ho tlosa ethanol e setseng ka tekanyo e kholo ka ho fetisisa.
Litaelo:
Viral RNA Isolation Kit Instruction Manual
