Blood SuperDirectᵀᴹ PCR Kit(UNG)-EDTA Blood Direct PCR Master Mix bakeng sa Genotyping of Blood
Litlhaloso
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMMix(UNG) -EDTA :Motheong oa 2×SuperEasyTMMix-EDTA, dUTP e sebelisoa sebakeng sa dTTP, le enzyme ea UNG e ka senyang template e nang le dUTP e kenngoa ka nako e le 'ngoe, e ka thibelang ka katleho ho silafala ha lihlahisoa tsa amplification tsa PCR.E na le D-Taq DNA Polymerase e fetotsoeng, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer le stabilizer, joalo-joalo. Boemong ba PCR, kenya feela mali, li-primers le ddH tse nepahetseng.2O ho 2 × SuperEasy e lumellanangTMMix(UNG) -EDTA e tla sebelisoa karabelong ea PCR.
6 × DNA Loading Buffer: The Loading Buffer ha e na SDS.Ho khothalletsoa ho sebelisa 6 × DNA Loading Buffer e kenyellelitsoeng le kit ha u etsa agarose gel electrophoresis e le hore u fumane liphello tse ntle tsa electrophoresis.U se ke ua sebelisa Loading Buffer e nang le SDS, ho seng joalo ho tla ba le mohatla o moholo oa khanya e khanyang tseleng nakong ea electrophoresis, e tla ama liphello tsa liteko.
Likarolo tsa Kit
2 × SuperEasyTMMotsoako(UNG)-EDTA |
6× DNA Loading Buffer |
Litaelo |
Likarolo le melemo
- Ha ho na tlhoko ea tlhoekiso ea DNA e jang nako e ngata le e theko e boima, ha ho kalafo ea pele, le tšebeliso e tobileng ea mali a felletseng joalo ka template ea PCR.
-Sistimi e na le matla a matla a ho holisa 'me e khona ho lemoha ka hloko liphatsa tsa lefutso tsa DNA le liphatlalatso tsa kantle ho naha maling.
-2 × SuperEasyTMMix(UNG) -EDTA e na le mamello e phahameng ea mali: ho fihla ho 45% maling a motho le 20% maling a toeba.
-Mohlala o koaletsoe ka botlalo, kahoo ha ho na lebaka la ho tšoenyeha ka tšilafalo ea sampole le liphetho tse fosahetseng tsa PCR.Anti-tšilafalo tsamaiso ea PCR 2 × SuperEasyTMMix(UNG) -EDTA, ka katleho e felisa tšilafalo e bakoang ke lihlahisoa tsa PCR, 'me e netefatsa ho nepahala le ho nepahala ha amplification.
Sesebelisoa sa Kit
E nehetsoe ho tsebahatsa PCR e tobileng ea mali a EDTA a anticoagulated.(Ho kenyeletsoa: mali a motho, mali a toeba, mali a khoho, mali a nonyana, mali a khomo, mali a ntja, joalo-joalo)
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
Sekhahla sena se bolokoa ho -20 ° C.
2 × SuperEasyTMMix(UNG) -EDTA,Ha e sebelisoa khafetsa, e ka boela ea bolokoa ho 4°C bakeng sa polokelo ea nako e khutšoanyane (sebelisa pele ho matsatsi a 10).
6× DNA Loading Buffer, E ka bolokoa ho 4°C kapa -20°C nako e telele.