Ka mokitlane o motle oa khoebo, tšebeletso e ntle ka mor'a thekiso le lisebelisoa tsa morao-rao tsa tlhahiso, re fumane botumo bo botle har'a bareki ba rona lefatšeng ka bophara bakeng sasete e felletseng ea li-reagents, Morero oa feme ea rona e lokela ho ba ho fana ka thepa ea boleng bo holimo ka theko e ntle ka ho fetisisa.Re ntse re lebelletse ho hlophisa mokhatlo le uena!
Ka mokitlane o motle oa khoebo, tšebeletso e ntle ka mor'a thekiso le lisebelisoa tsa morao-rao tsa tlhahiso, re fumane botumo bo botle har'a bareki ba rona lefatšeng ka bophara bakeng sasete e felletseng ea li-reagents, Re ipapisitse le thepa ea boleng bo holimo, theko ea tlholisano, le tšebeletso ea rona e felletseng, joale re bokelletse matla le boiphihlelo bo tšoanelehang, 'me re iketselitse botumo bo botle haholo tšimong.Hammoho le tsoelo-pele e tsoelang pele, ha re itlame feela ka khoebo ea malapeng ea Chaena empa hape le 'maraka oa machaba.E se eka u ka susumetsoa ke lintho tsa rona tsa boleng bo holimo le tšebeletso e chesehang.Ha re buleng khaolo e ncha ea melemo ea bobeli le ho hapa habeli.

Litlhaloso

50 Preps, 200 Preps

Likarolo tsa Kit

Likarolo le melemo

■ Tshebetso eohle e sebetsa mocheso oa kamore (15-25 ℃), ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Thepa eohle ha e na RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa ka metsotso e 11.
■ Polokeho: Ha ho hlokahale reagent ea tlhaho.
■ Boleng bo phahameng: RNA e hloekisitsoeng e hloekile haholo, ha e na protheine le litšila tse ling, ’me e ka kopana le liteko tse sa tšoaneng tse latelang.

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa liseleng tse hlahisitsoeng ka har'a lipoleiti tse 96, 24, 12 le 6 tsa seliba.

Phallo ea mosebetsi

Setšoantšo

Sets'oants'o sa betri ea gel ea agarose ea Cell Total RNA Isolation Kit se tšoere linomoro tse kaholimo tse fapaneng tsa lisele, 20μl bophahamo ba modumo, nka 2μl e hloekisitsoeng RNA 1%.

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) le 2-8 ℃ nako e telele (likhoeli tse 24).

Buffer cRL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho eketsa 2-hydroxy-1-ethanethiol(optional) g.Re ntse re lebelletse ho hlophisa mokhatlo le uena!
Fektheri ka kotloloho China Chemical Reagent, Research Chemical, E ipapisitse le thepa ea boleng bo holimo, theko ea tlholisano, le ts'ebeletso ea rona e felletseng, joale re bokelletse matla a tšoanelehang le boiphihlelo, 'me re iketselitse botumo bo botle haholo tšimong.Hammoho le tsoelo-pele e tsoelang pele, ha re itlame feela ka khoebo ea malapeng ea Chaena empa hape le 'maraka oa machaba.E se eka u ka susumetsoa ke lintho tsa rona tsa boleng bo holimo le tšebeletso e chesehang.Ha re buleng khaolo e ncha ea melemo ea bobeli le ho hapa habeli.