RT-qPCR Kit e tobileng
Tlhaloso ea Kit:
◮ Direct RT-qPCR Kit, e sebelisa Foreasy Reverse Transcriptase le Foreasy HS Taq DNA Polymerase e hlahisoang ke Foregene, e nang le buffer e ikhethang ea karabelo, e etsa hore kit ena e be le khanyetso e matla le e tsamaellanang, 'me e ka leka ka kotloloho ts'ebeliso ea sistimi ea Foregene Lysis joalo ka template.Setsi sena se ka etsa kopo bakeng sa lisebelisoa tsa ho bona tsa COVID-19 nucleic acid le nts'etsopele ea lisebelisoa tsa ho lemoha libaktheria tsa pathogenic tsa nucleic acid.
Tlhaloso
Direct RT-qPCR Kit e fana ka sephutheloana sa RT-qPCR se pakiloeng ka thoko le enzyme e sebetsang hantle haholo.motsoako (Taq, M-MLV, RI), e etsang hore e be bonolo bakeng sa ho theha lisebelisoa tsa morao-rao le sistimi.tokiso, ka har'a netefatso e bonolo.
Litlhaloso
500T, 50,000T
Likarolo tsa Kit
| Likahare tsa Kit (Sistimi ea karabelo ea 20 μL) | IM-05111-01 | IM-05112-01 |
| 500 T | 50,000 T | |
| Motsoako o otlolohileng oa Enzyme | 500 μL×1 | 50 ml×1 |
| 2 × Direct RT-qPCR Buffer | 1 mL×5 | 500 ml×1 |
| Taelo | 1 | 1 |
Mehato ea Ts'ebetso
Pele ho lokisoa ha reagent, li-reagents tsohle tse ka har'a kit li lokela ho qhibiliha ka mocheso oa kamore ebe li tsoakoa ka bonolo.
A: Lokisetsa of template le reagent
1. Lokisetsa template ea RNA e lokiselitsoeng (Ho kgothaletswa ho sebelisa Foregene Total RNA Isolation Kit series kit ho ntša le ho hloekisa template ea RNA) kapa sehlahisoa sa ho senya sehlahisoa (Ho khothalletsoa ho sebelisa tsamaiso ea Foregene Lysis), li-primers tse khethehileng (10 μM) le lisebelisoa tse ling tse amehang, sesebelisoa.
2. Beha 2× Direct RT-qPCR Buffer, RNase-Free ddH2O le 20× ROX Reference Dye(haeba e's hlokahala) ka lebokoseng le leqhoa, ho etsa hore tsena li qhibilihe ka tlhaho, 'me li kopane ka bonolo.
B: Lokisetsa of RT-qPCR tsamaiso
Tafole 1 : Lokisetsa of RT-qPCR stsamaiso
| Karolo | Bolumo | Khatiso ea ho qetela |
| 2 × Direct RT-qPCR Buffer | 10 μL | 1× |
| Motsoako o otlolohileng oa Enzyme | 1μL | |
| Primer ea pele(10 μM) | 0.8 μL | 50-900nM |
| Reverse Primer(10 μM) | 0.8 μL | 50-900nM |
| Probe(4 μM) | 1μL | 200nM |
| Setšoantšo (RNA kapa Lysate) | X μL | |
| 20 × ROX Reference Dye | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Kakaretso ea Bolumo | 20μL |
C: Beha the RT-qPCR karabelo lenaneo
Tafole 2 : RT-qPCR reaction lenaneo tlhophiso (bakeng sa mohlala: tse pedi-mohato mokhoa)
| Mohato | Mocheso | Nako | Lipotoloho | Litaba | |
| 1 | 50 ℃ | 15 mets | 1* | 1 | Phetolelo ea morao |
| 2 | 95℃ | 1 mets | 1 | Pre-denaturation | |
| 3 | 95℃ | 10 sec | 40-45 | Denaturation | |
| 60 ℃ | 30 metsotsoana 2* | Anneal/Katoloso | |||
Storage le Shelf bophelo
-The kit lokela ho bolokoa ka -20± 5℃.Nako ea ho sebetsa ke likhoeli tse 12.
- Qoba makhetlo a mangata a ho qhibiliha a leqhoa (<5 potoloho).
