ForeDirect RT-qPCR Kit
Tlhaloso ea Kit:
◮Bakeng sa ntlafatso ea nako ea 'nete ea RNA ka kotloloho ho tsoa ho likoleke tsa swab ntle le lits'ebetso tsa tlhoekiso ea RNA.Sesebelisoa sena se phethela lipotoloho tsa qRT-PCR nakong ea hora e le 'ngoe.Motsoako oa enzyme ke motsoako o ntlafalitsoeng oa Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Reaction Buffer e na le likarolo tsohle tse hlokahalang, ho kenyeletsoa likarolo tsa buffer tse ntlafalitsoeng, Mg2+, dUTP, le dNTPs.
Tlhaloso
ForeDirect RT-qPCR Kit e etselitsoe ho holisa RNA ka kotloloho ho tsoa ho pokello ea swab ntle le lits'ebetso tsa tlhoekiso ea RNA.Sesebelisoa sena se phethela lipotoloho tsa qRT-PCR nakong ea hora e le 'ngoe.Motsoako oa enzyme ke motsoako o ntlafalitsoeng oa Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Reaction Buffer e na le likarolo tsohle tse hlokahalang, ho kenyeletsoa likarolo tsa buffer tse ntlafalitsoeng, Mg2+, dUTP, le dNTPs.
Litlhaloso
100T
Likarolo tsa Kit
| Moemeli oa ho lokolloa ha Nucleic acid |
| Tšireletso ea RNA |
| 2 × RT-qPCR Buffer |
| Motsoako oa Enzyme(Taq&M-MLV) |
| Litaelo |
Sesebelisoa sa PCR
1. Hang ha li-tubes tsa PCR li se li kentsoe centrifuged ka nakoana, li behe ka har'a tanka ea sampole ea sesebelisoa sa amplification.
2. Thermal protocol
| Mohato | Mocheso | Nako | Lipotoloho | |
| 1 | Phetolelo ea morao | 50 ℃ | 15 mets | 1 |
| 2 | Pre-denaturation | 95℃ | 1 mets | 1 |
| 3 | Denaturation | 95℃ | 10 metsotsoana | 42 |
| 4 | Anneal/Katoloso | 60 ℃ | metsotsoana e 30 | |
Hlokomela:Letšoao la fluorescent le ile la fumanoa hang ka mor'a mohato oa ho atolosa oa potoloho ka 'ngoe.
3. Ka mor'a ho beha, boloka faele 'me u tsamaise lenaneo la karabelo.
Mehato ea Ts'ebetso
Pele ho lokisoa ha reagent, li-reagents tsohle tse ka har'a kit li lokela ho qhibiliha ka mocheso oa kamore ebe li tsoakoa ka bonolo.
Boitokisetso ba reagent
1. Lokisetsa Motsoako oa Release ea Nucleic Acid
| Karolo | Bolumo ka Sample kapa Taolo |
| Moemeli oa ho lokolloa ha Nucleic acid | 5μL |
| Tšireletso ea RNA | 0.5 μL |
2. Lokisetsa Motsoako oa Reaction
| Karolo | Bolumo ka Sample kapa Taolo |
| 2 × RT-qPCR Buffer | 15 μL |
| Motsoako oa Enzyme(Taq&M-MLV) | 1.5 μL |
| Li-Primers le Probes | 1μL |
Storage le Shelf bophelo
-The kit lokela ho bolokoa ka -20± 5℃.Nako ea ho sebetsa ke likhoeli tse 12.
- Qoba makhetlo a mangata a ho qhibiliha a leqhoa (<5 potoloho).
