Mohale oa PCR
Tlhaloso ea Kit:
◮Botšepehi bo phahameng: 6linako tsa enzyme e tloaelehileng ea Taq;
◮Ka potlako alebelo la ho ntlafatsa:lebelo la amplification ke 5-10sec/kb, e leng makhetlo a 6-12 a enzyme e tloaelehileng ea Taq;
◮Ho ikamahanya le maemo ho feta template: e ka holisa ka nepo litempele tse fapaneng tse rarahaneng tsa DNA tse nang le litaba tse phahameng tsa GC mme ho thata ho li holisa;
◮E phahamengMatla a ho holisa: palo ea lipotoloho tsa amplification e tlase ho feta ea enzyme e tloaelehileng ea Taq;
◮Smatlafala Mamello ya tikoloho: e behiloe ho 37°C ka beke, e boloka ho feta90%ts'ebetso;
◮E na le ts'ebetso ea 5'→ 3' DNA polymerase le 5'→ 3' exonuclease, ntle le ts'ebetso ea 3'→5' ea exonuclease.
Litlhaloso tsa Kit
Sehlahisoa sena se kenyelletsa moloko o mocha o ikhethang oa Foregene oa Taq——Forgene Taq Hero, dNTP e ntlafalitsoeng, MgCl2, buffer reaction, PCR reaction enhancer, optimizer le stabilizer.Mohale oa 2 × PCRTMSistimi ea Mix e na le mamello e phahameng ho li-inhibitors tsa PCR ho feta sistimi e tloaelehileng ea PCR Mix, 'me e khona ho sebetsana habonolo le ntlafatso ea PCR ea litempele tse fapaneng tse rarahaneng.Sistimi e ikhethang ea karabelo le ts'ebetso e phahameng ea Taq Hero li etsa hore karabelo ea PCR e be le bokhoni bo phahameng ba ho holisa, boits'oaro le kutlo.
Litlhaloso
Sehlopha sa Kit
PH-01013: 2× PCR MohaleTMKopanya 1.7ml ×3
PH-01014: 2× PCR MohaleTMKopanya 1.7ml × 6
PH-01015: 2× PCR MohaleTMKopanya 1.7ml ×24
Likarolo tsa Kit
2 × PCR HeroTMMotsoako o kenyelletsa:0.1U Taq Hero/μl, 400μM dNTPs, 20mM Tris-HCl(pH8.8), 100mM KCl, 5mM MgCl2, li-stabilizers tse ling le li-enhancer.
Likarolo le melemo
-Botšepehi bo phahameng:6linako tsa enzyme e tloaelehileng ea Taq;
- Ka potlako alebelo la ho ntlafatsa:lebelo la amplification ke 5-10sec/kb, e leng makhetlo a 6-12 a enzyme e tloaelehileng ea Taq;
-Ho ikamahanya le maemo ho feta template: e ka holisa ka nepo litempele tse fapaneng tse rarahaneng tsa DNA tse nang le litaba tse phahameng tsa GC mme ho thata ho li holisa;
- E phahamengMatla a ho holisa: palo ea lipotoloho tsa amplification e tlase ho feta ea enzyme e tloaelehileng ea Taq;
-Smatlafala Mamello ya tikoloho: e behiloe ho 37°C ka beke, e boloka ho feta90%ts'ebetso;
-E na le ts'ebetso ea 5'→ 3' DNA polymerase le 5'→ 3' ts'ebetso ea exonuclease, ntle le ts'ebetso ea 3'→ 5' ea exonuclease.
Sesebelisoa sa Kit
- Matlafatso ea PCR ea likhechana tsa DNA
- DNA labeling
- tatellano ea DNA
- PCR hammoho le mohatla
Polokelo le shelf bophelo
E ka bolokoa nako e telele ho -20 ° C;bakeng sa tšebeliso ea khafetsa, e ka bolokoa ho 4 ° C ka nako e khutšoanyane.
