Kakaretso ea Plasmid Mini Kit Kakaretso ea Plasmid DNA Extraction Miniprep Mini Kits
Tlhaloso ea Kit:
Ka potlako hloekisa plasmid DNA ea boleng bo holimo ho tsoa ho libaktheria tse fetotsoeng bakeng sa liteko tse tloaelehileng tsa baeloji ea limolek'hule joalo ka phetoho le tšilo ea enzyme.
◮Tlosa RNA ntle le ho eketsa RNase
◮E loketseng- centrifugation e etsoa ka mocheso oa kamore
◮Ka potlako- ts'ebetso e ka phethoa ka metsotso e 20
◮Sireletsehile- ha ho na reagent ea tlhaho e sebelisoang
◮Bohloeki bo phahameng—OD260/280≈1.7-1.9
Litlhaloso tsa Kit
Lihlahisoa tsa FOREGENE'S nucleic acid extraction and purification series li ka ntšoa le ho hloekisoa ho li-nucleic acid tsa boleng bo holimo ho tsoa mehloling e fapaneng le lisampole tsa likarolo tse rarahaneng.Lihlahisoa tsa DNA Extractive li thehiloe holim'a DNA-Feela silica membrane purification columns le li-reagents tse ikhethang tse ka hloekisang DNA ea boleng bo phahameng ho tsoa mefuteng e fapaneng.
The General Plasmid Mini Kit e sebelisa theknoloji ea kholomo ea tlhoekiso ea DNA-Feela le mokhoa o sebetsang oa SDS lysis ho fumana plasmid ea boleng bo holimo ho libaktheria nakong ea metsotso e 20.DNA-Feela silica gel membrane e sebelisitsoeng kholomong ea spin ke ntho e ncha e ikhethang ea FOREGENE, e ka bapatsang DNA hantle le ka ho hlaka, le ho tlosa RNA, liprotheine tse sa hloekang, li-ion le metsoako e meng ea lintho tse phelang liseleng ho isa tekanyong e kholo.Boholo bo tlamang ba DNA ea plasmid ea kholomo ea tlhoekiso ea DNA-Feela ke 60μg DNA.Palo ea plasmid DNA e hlileng e nkiloeng e amana le boholo ba tharollo ea baktheria, palo ea likopi tsa plasmid (kopi e phahameng, kopi e tlaase), mathata, maemo a setso le lintlha tse ling.
Litlhaloso
| Kakaretso ea Plasmid Mini Kit | |||
| Likahare tsa Kit | DE-01001 | DE-01002 | DE-01003 |
| 50T | 100T | 250T | |
| Buffer S1 | 10ml | 20ml | 50ml |
| Buffer S2 | 10ml | 20ml | 50ml |
| Buffer S3 | 25ml | 50ml | 125ml |
| Sekhahla sa PW | 25ml | 50ml | 125ml |
| Buffer WB2 | 15ml | 30ml | 75ml |
| Buffer EB | 10ml | 20ml | 50ml |
| DNA-Feela Kholomo | 50 | 100 | 250 |
| Bukana | 1 | 1 | 1 |
Likarolo le melemo
- Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho plasmid DNA ntle le ho eketsa RNase nakong ea teko, ho qoba ho silafatsoa ke laboratori ka RNase e sa tloaelehang.
- Lebelo le potlakileng: Ho ntšoa ha Plasmid DNA ho ka phethoa nakong ea metsotso e 20.
- E Sireletsehile: Ha ho hlokahale ho ntšoa ha reagent ea organic.
- Boleng bo phahameng: Plasma e fumanoeng ka ho ntšoa ha e na RNA, ha e na RNase le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
Sesebelisoa sa Kit
Ho ntšoa le ho hloekisa DNA ea Plasmid bakeng sa libaktheria tsa Gram-negative.
Phallo ea mosebetsi
Setšoantšo
Polokelo le shelf bophelo
Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ℃);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ka 2–8℃.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka bateng ea metsi ea 37 ℃ metsotso e 10 ho qhala metsi, 'me u e kopanye pele u e sebelisa.
