China Plant Peo(Nnyane le Hare) Direct PCR Kit I-UNG(ntle le Sampling Tools) Moetsi le Mofani |Foregene

Peo ea Jala(E Nnyane le e Mahareng) Direct PCR Kit I-UNG(ntle le Lisebelisoa tsa Sampling)

Peo ea Semela(E Nnyane le e Bohareng) Direct PCR Kit I-UNG(ntle le Lisebelisoa tsa Sampling) Sets'oants'o se Tsoang

Tlhaloso ea Kit:

Tšebeliso e tobileng ea lysate ea peo e le template bakeng sa ho hōlisa PCR, ha ho na ho ntšoa ka thōko ho DNA, ka potlako le ka kutloisiso e phahameng.Lenaneo la UNG le khahlanong le tšilafalo le ekelitsoe ho thibela mekhoa e fosahetseng.

◮Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.

◮Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.

◮Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, 'me ho buuoa ho bonolo.

◮Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Litlhaloso

Sehlahisoa sena se sebelisa mokhoa o ikhethang oa lysis reaction system, o ka lokollang DNA ea genomic ka potlako ho tloha lisampole tsa peo ea limela tse nang le polysaccharide e tlaase le polyphenol content (e kang: raese, koro, koae, poone, linaoa tsa soya, joalo-joalo) bakeng sa ho sebelisoa ka liketso tsa PCR, kahoo e loketse ka ho khetheha Teko e kholo ea liphatsa tsa lefutso.E le ho fihlela litlhoko tsa liteko tse fapaneng tsa ho lemoha, setsi sena se ka sebelisa mefuta e mengata ea lisampole (joalo ka peo e felletseng, maqeba a manyane, kapa lisampole tsa fatše tsa lipeo tse ngata) bakeng sa liteko.Har'a tsona, bakeng sa lisampole tsa peo tse ntseng li hloka ho mela, o ka khetha ho khaola maqeba a manyane (1-5mg) ntle le peo ea embryo bakeng sa liteko;bakeng sa liteko tse hlokang ho etsoa sampole ka palo e kholo ea lisampole, o ka khetha ho kopanya lisampole tse ngata le ho li kopanya.Ka mor'a ho sila ka likaroloana tse nang le bophara ba hoo e ka bang 0.5mm, teko e ka etsoa (bonyane 0.1% ea sampuli e reretsoeng e ka fumanoa).

Litlhaloso

50T,200T,500T,2000T

Likarolo tsa Kit

Seed Seed Direct PCR Kit I-UNG Peo ea Jala (E Nnyane le e Mahareng) Direct PCR Kit-UNG
Sebopeho sa Kit		TP-0312T	TP-03121	TP-03122	TP-03123
Sebopeho sa Kit		50 T	200 T	500 T	2000 T
Karolo ea I	Buffer SP1	10ml	40ml	100ml	400ml
	Buffer SP2	3ml	10ml	25ml	100ml
	6× DNA Loading Buffer	1.5ml	1.5ml	1.5ml	1.5ml×4
Karolo ea II	2× Peō ea PCR e Bonolo^TMMotsoako(UNG)	500μl	1mlx2	1.7ml×3	1.7ml×12
Bukana		1	1	1	1

Likarolo le melemo

- Ha ho na tlhoko e jang nako ebile e theko e boima ea tlhoekiso ea DNA e hlokahalang.

- Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.

- Ha ho na liphekolo tse khethehileng tse kang ho sila le ho sila tse hlokahalang, 'me ts'ebetso e bonolo.

- Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.

- Anti-tšilafalo tsamaiso ea PCR 2× Peō PCR Bonolo^TMMix (UNG) e ka felisa ka katleho tšilafalo e bakoang ke lihlahisoa tsa PCR le ho netefatsa ho nepahala le ho nepahala ha amplification.