RT-PCR ke theknoloji e kopanyang reverse transcript RT ea RNA le polymerase chain amplification (PCR) ea cDNA.Theknoloji ea RT-PCR e na le kutloelo-bohloko ebile e na le mekhoa e mengata, 'me e ka sebelisoa ho lemoha boemo ba liphatsa tsa lefutso ka liseleng / liseleng, litaba tsa likokoana-hloko tsa RNA liseleng, le ho kopanya ka ho toba tatellano ea cDNA ea liphatsa tsa lefutso tse itseng.
MOLEMO OA MONA:
✱ Foregene's RT kit e na le botsitso bo matla ho 50-65 ℃ mocheso oa karabelo (65°C e ka bula boemo ba bobeli ba RNA ho etsa hore phetiso e fetoletsoeng e tsoele pele hantle)
✱ Mamello e matla, e ka mamella ethanol e itseng le letsoai la guanidine
| Sub-Letoto | Lebitso la Sehlahiswa | Litlhaloso | Nomoro ea lethathamo | Maemo a polokelo |
| PCR Mix Series | 5ml | PH-01013 | -20 ℃ | |
| 40ml | PH-01015 | |||
| 5ml | PH-01013-B | -20 ℃ | ||
| 40ml | PH-01015-B | |||
| 2 × Mokhoa o phahameng oa PCR reaction premix system | 1ml | DP-20031 | -20 ℃ | |
| 3×1.7ml | DP-20032 | |||
| 3×1.7ml | DP-20032-B | -20 ℃ | ||
| 50ml | DP-20034-B | |||
| 2 × Potlako ea PCR reaction premix system 2 × PCR Mix | 1ml | DP-20041 | -20 ℃ | |
| 3×1.7ml | DP-20042 | |||
| 500 × 20μl rxns | QP-01012 | -20 ℃ | ||
| 2000 × 20μl rxns | QP-01014 | |||
| 500 × 20μl rxns | QP-01022 | -20 ℃ | ||
| 2000 × 20μl rxns | QP-01024 | |||
| Letoto la RT | 25 × 20μl Liketso | RT-01011 | -20 ℃ | |
| 100 × 20μl Karabelo | RT-01012 | |||
| (Master Premix bakeng sa motsoako oa pele oa cDNA bakeng sa nako ea sebele ea PCR) | 200 × 10μl Karabelo | RT-01022 | -20 ℃ | |
| 800 × 10μl Karabelo | RT-01023 | |||
| Master Premix bakeng sa motsoako oa pele oa cDNA bakeng sa Nako ea 'Nete PCR e nang le gDNase | 100 × 20μl Karabelo | RT-01032 | -20 ℃ | |
| Mohale oa Lnc-RTTM Ke(Ka gDNase)(Super Premix bakeng sa motsoako oa pele oa cDNA ho tsoa ho lncRNA) | 25 × 20μl Karabelo | RTL-09098 | -20 ℃ | |
| 50 × 20μl Karabelo | RTL-09099 | |||
| Letoto la RT-PCR | 100 rxns | RT-02011 | -20 ℃ | |
| 500 rxns | RT-02012 | |||
| 100 rxns | RT-02021 | -20 ℃ | ||
| 500 rxns | RT-02022 | |||
| Letoto la RT-qPCR | 100 rxns | RT-02111 | -20 ℃ | |
| 500 rxns | RT-02112 | |||
| 100 rxns | RT-02131 | -20 ℃ | ||
| 500 rxns | RT-02132 | |||
| Letšoao la DNA | 50 Preps | DL-20011 | 4℃ | |
| 200 Preps | DL-20012 | |||
| 50 Preps | DL-30011 | 4℃ | ||
| 200 Preps | DL-30012 |
