Lnc-RT Heroᵀᴹ I(With gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA)
Litlhaloso
25×20μl Rxns, 50×20μl Rxns
Lnc-RT Hero TM I (Ka gDNase) ke mokhoa oa ho fetolela ka morao o etselitsoeng lncRNA ka potlako ho tlosa tšilafalo ea DNA ea genomic.5 × gDNase Mix e ka tlosa ka potlako genome e setseng ho RNA ho 42 ° C bakeng sa metsotso ea 2, ka katleho e qoba ho kena-kenana le genome ho liphello tsa qPCR.
5×L-RT HeroTM Mix e na le Foregene LncRNA Reverse Transcriptase e entsoeng ka ho khetheha ke Foregene, e leng mofuta o mocha oa reverse transcriptase ka ho khetheha bakeng sa lncRNA le li-template tse ling tse telele tsa RNA tse telele, tse nang le kamano e matla ea RNA le bokhoni bo phahameng ba ho rekota.Sistimi e ntlafalitsoeng e etsa hore sekhahla sa khatiso e khutleleng e potlake 'me e khone ho ngola litempele tsa RNA habonolo ka litaba tse phahameng tsa GC le sebopeho se rarahaneng sa bobeli.The strand cDNA synthesis ea pele e ka phethoa ka 42 ° C ka metsotso e 15.
Likarolo tsa Kit
| 5× gDNase Mix |
| 5× Motsoako oa L-RT HeroTM |
| RNase-Free ddH2O |
| Litaelo |
Likarolo le melemo
■Bokhoni bo nepahetseng ba ho tlosa gDNA, e ka tlosa gDNA ka template nakong ea metsotso e 2.
■ E khona ho fetola phetiso ea lncRNA hantle.Ha sehlahiswa sa kgatiso e kgutlang se le tlasa qPCR, boleng ba Ct bo tlase ho feta ba sistimi e tlwaelehileng ya reverse transcript.
■ Sistimi e sebetsang hantle ea reverse transcript, ho nka metsotso e 15 feela ho phethela tšebetso ea cDNA ea pele.
■ Lithempleite tse rarahaneng: lithempleite tse nang le litaba tse phahameng tsa GC le sebopeho sa bobeli se rarahaneng le tsona li ka khutlisoa ka katleho e phahameng.
■ High-sensitivity reverse transcription system, litempele tsa pg-level le tsona li ka fumana cDNA ea boleng bo holimo.
■ Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso o nepahetseng oa karabelo ke 42℃, 'me e ntse e na le ts'ebetso e ntle ea ho fetolela ho 50℃.
Sesebelisoa sa Kit
lncRNA e amanang le tlhahlobo ea bongata.
lncRNA tlhahlobo e felletseng ea palo.
E ka sekaseka ka potlako le ka nepo trace RNA joalo ka vaerase ea RNA.
Phetolelo ea morao ea litempele tsa RNA tse nang le litaba tse phahameng tsa GC kapa sebopeho se rarahaneng sa bobeli.
Phallo ea mosebetsi
Storage le Shelf bophelo
The kit lokela ho bolokoa ka -20°C. Boloka sehlahisoa ka sehatsetsing se sa khaotseng sa mocheso ho -20°C hang ka mor'a ho fumana.Haeba maemo a polokelo a loketse, sehlahisoa se ke ke sa nyenyefatsa ts'ebetso leha e le efe nakong ea selemo sa 1 sa ho netefatsa.
Tataiso ea Tlhahlobo ea Mathata
The following is an analysis of the problems that may be encountered in the use of Lnc-RT HeroTM I (With gDNase) series kits, hoping to be helpful to your experiments. In addition, we have dedicated technical support to help you with other experimental or technical problems beyond the operating instructions and questions. If you have any needs, please contact us: 028-83361257 or E-mail: Tech@foregene.com.
Non-amplification e khethehileng
1. Moralo o sa utloahaleng oa primer
Keletso: Etsa li-primers ho latela melao-motheo ea moralo oa pele.
2. Masalla a Genome.
Tlhahiso: Etsa bonnete ba hore mohato oa pele oa mocheso oa ho tlosa gDNA ke 42 ° C, 'me nako ea ho arabela e ka boela ea eketsoa ho 5min.
Ha ho lets'oao la ho holisa ka RT-qPCR
1. RNA e senyehile.
Keletso: Thepa ea RNA e ntšoa e lokela ho ba e ncha ka hohle kamoo ho ka khonehang, 'me ho sebelisoe RNA ea boleng bo holimo le e hloekileng.
2. RNA e na le li-inhibitors.
Keletso: Reverse transcription inhibitors ka kakaretso e kenyelletsa SDS, guanidine salts, EDTA, joalo-joalo Ho khothalletsoa ho hlatsoa precipitate ea RNA ka 70% ethanol ho tlosa li-inhibitors.
3. Litaba tsa moralo oa mantlha.
Keletso: Ho ea ka molao-motheo oa moralo oa pele, hlophisa bocha li-primers bakeng sa tlhahlobo.
Sehlopha se shebiloeng se hlaha taolong e se nang letho
1. Tšilafalo ea lisebelisoa tsa ts'ebetso kapa li-reagents.
Keletso: Lisebelisuoa tsohle kapa lisebelisoa tsa teko li lokela ho etsoa ka mokhoa o ikemetseng.E-ba hlokolosi 'me u be bonolo ha u tšoara ho thibela lisampole tsa DNA hore li se ke tsa kenngoa ka har'a pipette kapa tsa tšolloa ka har'a tube ea centrifuge.
2. Tšilafalo e etsahetse nakong ea ho lokisetsa mokhoa oa ho arabela oa PCR.
Keletso: Ela hloko litemoso tse hlokahalang ha u tšoara, joalo ka: ho roala liatlana tsa latex, u sebelisa malebela a sefe.Sebelisa Nako ea 'Nete ea PCR Mix ka har'a sistimi e thibelang tšilafalo.
3. Li-primers li senyehile.
Tlhahiso: Sebelisa electrophoresis ea SDS-PAGE ho bona hore na li-primers li senyehile, 'me u nke sebaka ka lisebelisoa tse ncha bakeng sa liteko tsa ho lemoha fluorescence.
Buka ea litaelo:
Lnc-RT HeroTM I(With gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA) Buka ea Taelo
