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EndoFree Maxi Plasmid Kit (Spin Column)

Tlhaloso ea Kit:

Ts'ebetso eohle ea ho ntša e nka hora e le 'ngoe feela, ho netefatsa ts'ebetso e bonolo le e potlakileng.

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Tlhaloso

EndoFree Maxi Plasmid Kit e sebelisa mokhoa o ikhethileng oa silika oa adsorption ho tlama DNA ea plasmid ka nepo le ka ho khetheha.Ka ho kopanya Endotoxin Removal Buffer P4 le EndoFree Maxi Filtration Columns, endotoxin, liprotheine le litšila tse ling li ka tlosoa ka katleho.Ts'ebetso eohle ea ho ntša e nka hora e le 'ngoe feela, ho netefatsa ts'ebetso e bonolo le e potlakileng.

Palo ea libaktheria tse khothalelitsoeng bakeng sa ts'ebetso e 'ngoe le e' ngoe: Bakeng sa plasmid ea kopi e phahameng, ho khothaletsoa ho sebelisa 100 ml ea media media ea libaktheria, 'me chai eo hangata e leng 500-1500 μg.Bakeng sa plasmid ea kopi e tlase, 200 ml media media culture e khothaletsoa ho hlahisa li-plasmids tse ka bang 200-600 μg.

Likarolo tsa Kit

Sehlahisoa Likaroloana

D909-E(Lipehelo tse 10)
Buffer BL 30 ml
Buffer P1 100 ml 
Buffer P2 100 ml 
Buffer P4 100 ml
Sekhahla sa DW2 44 ml
Buffer EB 30 ml
RNase A(10 mg/ml) 1 ml
DNAPure Maxi Spin Columns 10 likhomphutha
Siringe ea FinePure Maxi Filter 10 likhomphutha
50 ml Li-tube tsa Pokello 10 likhomphutha

Ho fumanoa ha Plasmid DNA Concentration le Bohloeki

Bohloeki le mahloriso a plasmid e ntšitsoeng DNA e ka bonoa ka agarose gel electrophoresis le ultraviolet spectrophotometer.Boleng ba OD260 ba 1 bo tsamaellana le hoo e ka bang 50 μg/ml ea DNA e likhoele habeli.Theko ea OD260/OD280 ea DNA e hloekisitsoeng ea plasmid hangata e ka har'a 1.7-1.9.DNA e hloekisitsoeng ea plasmid e ka sebelisoa ka ho toba litekong tse nang le litlhoko tse phahameng tsa bohloeki, tse kang ho fetisetsa lisele kapa liteko tsa vivo.

Sesebelisoa sa Kit

DNA ea plasmid e ntšitsoeng ke kit ena e ka sebelisoa ts'ebetsong e fapaneng ea tloaelo, ho kenyelletsa le tšilo ea enzymatic, PCR, tatellano, ligation, phetoho hammoho le phetiso ea mefuta e fapaneng ea lisele.

Storage le Shelf bophelo

EndoFree Maxi Plasmid Kit e ka bolokoa mocheso oa kamore (15-25 ° C) tlas'a maemo a omileng ho fihlela likhoeli tse 12.Kenya RNase A ho Buffer P1 pele o sebelisoa pele, kopanya hantle 'me u boloke ho 2-8°C.Motsoako o ka bolokoa ka mokhoa o tsitsitseng ho feta likhoeli tse 6.

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