Mouse Tail DNA Mini Kit Genomic DNA Extraction Kits ho tloha Mouse Tail
Tlhaloso ea Kit:
Thepa e potlakileng ka ho fetisisa lefatšeng ea ho ntša DNA ea mohatla oa mouse, e ka ntšang DNA ea boleng bo holimo mohatleng oa toeba nakong ea hora e le 'ngoe.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
Tlhaloso
Ka lebaka la sebopeho le thepa ea mohatla oa mouse ka boeona, ho ntšoa ha mouse mohatla genomic DNA e 'nile ea tšoenya bafuputsi: e' ngoe ke hore nako ea ts'ebetso ea mohatla oa mouse e telele 'me DNA ea genomic e senyeha habonolo, e' ngoe e thata ho sebetsana le ho hloeka ha DNA e nkiloeng ea genomic e tlaase.
Setsi sena se sebelisa DNA-Feela Column e ka tlamang DNA ka ho khetheha, Foregene Protease e ncha le sistimi e ikhethang ea buffer, e ka chekang lisampole tsa mohatla oa litoeba nakong ea metsotso e 45, kahoo e fokotsa ho senyeha ha DNA ea genomic le ho khutsufatsa nako ea ts'ebetso ea sampole.Thepa e ka ntša 10-20μg ea DNA ea boleng bo holimo, e hloekileng ea genomic ho tloha 0.5-1cm mohatla oa bana kapa oa batho ba baholo ka hare ho 60-90min.
Thepa ea matrix ea silica gel ea DNA feela e sebelisitsoeng mokokotlong oa spin ke thepa e ncha e ikhethang ea k'hamphani, e ka bapang DNA ka nepo le ka ho khetheha.Bokhoni bo boholo ba ho bapatsa bakeng sa DNA ke 80μg.Sistimi e ikhethang ea buffer le elution e ka eketsa ho tlosoa ha RNA, liprotheine tse sa hloekang, li-ion le metsoako e meng ea tlhaho ka seleng.Sekhechana sa DNA se nkiloeng se seholo, se hloekile, se tsitsitse ebile se ka tšeptjoa ka boleng, 'me boholo ba sekhechana sa DNA bo tsitsitse hoo e ka bang 23kb.
Kit e ka boela ea sebelisoa ho ntša lisele tse ling tsa mouse ka ntle ho mohatla oa mouse, ho akarelletsa le litsebe tsa mouse, mesifa ea mouse, sebete sa mouse le lisele tse ling, e le hore kit e le 'ngoe e ka sebelisoa bakeng sa merero e mengata le ho finyella litlhoko tsa basebelisi bakeng sa liteko tse fapaneng.
Litlhaloso
50 Preps, 100 Preps, 250 Preps
Likarolo tsa Kit
| Buffer TL1 |
| Buffer TL2* |
| Buffer PW* |
| Buffer WB |
| Buffer EB |
| Foregene Protease Plus |
| DNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
-Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho qoba laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
-Lebelo le potlakileng: Foregene Protease Plus e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso e 40.
-E bonolo: The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlaase oa centrifugation kapa ethanol precipitation ea DNA.
-Tshireletso: Ha ho hlokahale ho ntšoa ha reagent ea organic.
-Boleng bo phahameng: DNA e nkiloeng ea genomic e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse sa tšoaneng.
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha DNA ea genomic ho tloha mehatleng e mecha kapa e leqhoa e meholo le ea toeba (e boetse e sebelisetsoa ho hloekisa mouse kapa mesifa ea rat le mouse ea tsebe ea genomic DNA).
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
-Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho 2-8°C.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.
-Forene Protease Plus tharollo e na le foromo e ikhethang, e sebetsa ha e bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);e bolokiloe ka 4 ° C, ts'ebetso le botsitso li tla ba molemo, kahoo ho kgothaletswa ho e boloka ho 4 ° C, hopola hore u se ke ua e boloka ho -20 ° C.
