FFPE DNA Isolation Kit DNA Extraction Purification Kit ho tloha FFPE Samples
Tlhaloso ea Kit:
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa ho lithane tse kentsoeng ke parafun tse kang likarolo tsa parafun le liboloko tsa parafun.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
Tlhaloso
Setsi sena se etselitsoe ho ntša le ho hloekisa DNA ea boleng bo holimo ho tsoa ho lisele tse tsitsitseng tsa formaldehyde kapa lithane tse kentsoeng ka parafine.
DNA ea genomic ea sampole e kentsoeng ka parafine e tla senyeha ka lebaka la nako ea polokelo kapa tikoloho, e leng ho etsang hore ho be thata ho ntša DNA ea genomic.Setsi sena se nka mokhoa o ikhethileng o ntlafalitsoeng, o loketseng ho ntša DNA ea genomic ho lisampole tse kentsoeng ka parafine ka sekhahla se phahameng sa katleho.Ts'ebetso eohle ha e hloke ho sebelisa chefo ea phenol, chloroform kapa pula ea isopropanol.
Setsi sena se sebelisa kholomo e ka kopanyang DNA ka ho khetheha, Foregene Protease Plus e ncha, le sistimi e ikhethang ea buffer, e ka ntšang DNA ea boleng bo holimo e kentsoeng ka parafine nakong ea lihora tse 2-5.
Litlhaloso
50 Preps, 100 Preps, 250 Preps
Likarolo tsa Kit
| Buffer FL1 |
| Buffer FL2* |
| Buffer PW* |
| Buffer WB |
| Buffer EB |
| Foregene Protease Plus |
| DNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
-Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho qoba laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
-Lebelo le potlakileng: Foregene Protease Plus e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa ka hare ho lihora tse 2-5.
-Bonolo: centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4°C centrifugation ea mocheso o tlase kapa pula ea ethanol ea DNA.
-Tshireletso: ha ho hlokahale ho ntšoa ha reagent ea organic.
-Boleng bo phahameng: Likaroloana tse nkiloeng tsa genomic DNA li kholo, ha ho na RNA, ha ho RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse fapa-fapaneng.
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha DNA ea genomic ho tloha lisampoleng tse tsitsitseng tsa formaldehyde le disampole tse kentsoeng ka parafine.
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
-Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho 2-8°C.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o kenya tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.
-Forene Protease Plus tharollo e na le foromo e ikhethang e sebetsang ha e bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);e sebetsa haholo ebile e tsitsitse ha e bolokiloe ho 4 ° C, kahoo ho kgothaletswa ho e boloka ho 4 ° C, hopola hore o se ke oa e boloka ho -20 ° C.
