Barekisi ba Phahameng ba China ba Iketselitseng PCR Nucleic Acid Sistimi, Lisebelisoa tsa ho Nkoa ha Acid Nucleic
Tlhaloso ea Kit:
RNase-Mahala
E sebetsa ka mocheso oa kamore (15-25 ℃)
Ka DNA-Cleaning Column
Kotulo e phahameng ea RNA
Ka potlako: Qetella ho ntša ka mor'a metsotso e 11
Polokeho: Ha ho na Organic Chemical
Re natefeloa ke botumo bo botle ka mokhoa o makatsang har'a bareki ba rona bakeng sa thepa ea rona ea boleng bo holimo, lebelo le mabifi hammoho le ts'ehetso e sebetsang ka ho fetisisa bakeng sa Top Suppliers China Automated PCR.Ho ntšoa ha Nucleic AcidSistimi, Lisebelisoa tsa ho Rata Nucleic Acid, Tšireletseho ka lebaka la boqapi ke tšepiso ea rona ho e mong.
Re natefeloa ke botumo bo botle ka tsela e makatsang har'a bareki ba rona bakeng sa thepa ea rona ea boleng bo holimo, lebelo le mabifi hammoho le ts'ehetso e sebetsang hoChina Nucleic Acid Extract Machine, Ho ntšoa ha Nucleic Acid, Re fana ka litšebeletso tsa setsebi, karabo e potlakileng, ho fana ka nako, boleng bo botle le theko e ntle ho bareki ba rona.Khotsofalo le mokitlane o motle ho moreki e mong le e mong ke tsona tse tlang pele bophelong ba rona.Re shebana le lintlha tsohle tsa ts'ebetso ea odara bakeng sa bareki ho fihlela ba fumane litharollo tse bolokehileng le tse utloahalang ka litšebeletso tse ntle tsa thepa le litšenyehelo tsa moruo.Ho ipapisitse le sena, lihlahisoa tsa rona le tharollo li rekisoa hantle haholo linaheng tsa Afrika, Middle East le Asia Boroa-bochabela.Ho latela filosofi ea khoebo ea "moreki pele, tsoela pele", re amohela bareki ba tsoang hae le kantle ho naha ho sebelisana le rona.
Tlhaloso
50 Preps, 200 Preps
Setsi sena se sebelisa "spin column le formula" e ntlafalitsoeng ke Foregene, e ka ntšang RNA ea boleng bo holimo le ea boleng bo holimo liseleng tse entsoeng ka lipoleiti tse 96, 24, 12 le 6.
Thepa e fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo supernatant le cell lysate, ho tlama le ho tlosa DNA ea genomic.Opereishene e bonolo ebile e boloka nako.
Kholomo ea RNA-feela e ka tlama RNA hantle ka foromo e ikhethang.Palo e kholo ea disampole e ka sebetswa ka nako e le nngwe.
Likarolo tsa Kit
| Sebopeho sa Kit | RE-03111 | RE-03114 |
| 50 T | 200 T | |
| Buffer cRL1* | 25 ml | 100 ml |
| Buffer cRL2 | 15 ml | 60 ml |
| Buffer RW1* | 25 ml | 100 ml |
| Buffer RW2 | 24 ml | 96 ml |
| RNase-Free ddH2O | 10 ml | 40 ml |
| RNA-Feela Kholomo | 50 | 200 |
| DNA-Ho hloekisa Column | 50 | 200 |
| Taelo | 1 | 1 |
*Ka kopo, roala liatlana 'me u nke mehato ea ts'ireletso nakong ea ts'ebetso kaha Buffer cRL1 le Buffer RW1 li na le matsoai a chaotropic a halefisang.
Likarolo le melemo
■ Tshebetso eohle e sebetsa mocheso oa kamore (15-25 ℃), ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Thepa eohle ha e na RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa ka metsotso e 11.
■ Polokeho: Ha ho hlokahale reagent ea tlhaho.
■ Boleng bo phahameng: RNA e hloekisitsoeng e hloekile haholo, ha e na protheine le litšila tse ling, ’me e ka kopana le liteko tse sa tšoaneng tse latelang.
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa liseleng tse hlahisitsoeng ka har'a lipoleiti tse 96, 24, 12 le 6 tsa seliba.
Phallo ea mosebetsi
Setšoantšo
Sets'oants'o sa betri ea gel ea agarose ea Cell Total RNA Isolation Kit se tšoere linomoro tse kaholimo tse fapaneng tsa lisele, 20μl bophahamo ba modumo, nka 2μl e hloekisitsoeng RNA 1%.
Polokelo le shelf bophelo
Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele (likhoeli tse 24).
Buffer cRL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho kenya 2-hydroxy-1-ethanethiol(optional).Re thabela botumo bo botle ka tsela e makatsang har'a bareki ba rona bakeng sa thepa ea rona ea boleng bo holimo, e mabifi hammoho le tšehetso e sebetsang ka ho fetisisa bakeng sa Top Suppliers China Automated PCR.Ho ntšoa ha Nucleic AcidSistimi, Lisebelisoa tsa ho Rata Nucleic Acid, Tšireletseho ka lebaka la boqapi ke tšepiso ea rona ho e mong.
Top SuppliersChina Nucleic Acid Extract Machine, Nucleic Acid Extraction, Re fana ka litšebeletso tsa litsebi, karabo e potlakileng, ho fana ka thepa ka nako, boleng bo botle le theko e ntle ka ho fetisisa ho bareki ba rona.Khotsofalo le mokitlane o motle ho moreki e mong le e mong ke tsona tse tlang pele bophelong ba rona.Re shebana le lintlha tsohle tsa ts'ebetso ea odara bakeng sa bareki ho fihlela ba fumane litharollo tse bolokehileng le tse utloahalang ka litšebeletso tse ntle tsa thepa le litšenyehelo tsa moruo.Ho ipapisitse le sena, lihlahisoa tsa rona le tharollo li rekisoa hantle haholo linaheng tsa Afrika, Middle East le Asia Boroa-bochabela.Ho latela filosofi ea khoebo ea "moreki pele, tsoela pele", re amohela bareki ba tsoang hae le kantle ho naha ho sebelisana le rona.
