Foregene DNA Identification System Y Plus (Non-Extraction)
Tlhaloso ea Kit:
ForegeneSistimi ea ho tsebahatsa DNA Y Plus e sebelisa theknoloji ea mebala e tšeletseng ea ho ngola fluorescent ho holisa 20 Y chromosome core loci, 15 preferred loci, 6 alternative loci le 3 Y indel loci ka nako e le 'ngoe..
Tlhaloso
ForegeneSistimi ea ho tsebahatsa DNA Y Plus e sebelisa theknoloji ea mebala e tšeletseng ea ho ngola fluorescent ho holisa 20 Y chromosome core loci, 15 preferred loci, 6 alternative loci le 3 Y indel loci ka nako e le 'ngoe..
Litaba tsa Kit
Tokisetso ea tsamaiso ea amplification
Theho hlophisoatsa stloaelehileng(mahala)Sistimi ea amplification
| Cbahanyetsi | 25 μltsamaiso (μl) | Sistimi ea 10μl (μl) | |
| Master Mix | 4 × Master MixVII | 6.25 | 2.5 |
| 5×Y Plus Primer Mix | 5.0 | 2.0 | |
| Metsi a deionized | 13.75 | 5.5 | |
| Bthupa letheba | Bophara ba 1.2mm | Bophara ba 1.2mm | |
| Tmolumo oa otal reaction | 25 μl | 10 μl | |
Tlhahlobo ea Lintlha
Kerafo e Ekelitsoeng 1:'Mapa oa Allelic Ladder Typing
Kerafo e Ekelitsoeng 2:Tekanyetso ea ho thaepa ea DNA ea 9948'mapa
Reagent Storage
1. Ka kopo boloka ka tlase ho -20 ° C (Haeba e sa sebelisoe nakoana), ka mor'a ho amohela lisebelisoa tse leqhoa le leqhoa le omileng kapa liphutheloana tsa leqhoa la gel.
2. Ka kopo boloka karolo ea pre-reaction 4 × premix VII ho -20 ° C, ka mor'a hore kit e ntšoe hore e sebelisoe, 'me li-reagents tse ling tse setseng li bolokiloe ho 4 ° C ho qoba ho ba leqhoa khafetsa le ho qhibiliha.Haeba tekanyo e le 'ngoe e le nyane, ho kgothaletswa ho boloka ho -20 ° C ka mor'a aliquoting.
3. Boloka likarolo ka mor'a karabelo ho 4 ° C, qoba ho qhoqhoa khafetsa le ho qhibiliha, 'me u se ke ua ama li-reagents pele u arabela ho qoba ho silafala.
