RT Easyᵀᴹ II Master Premix bakeng sa motsoako oa pele oa cDNA bakeng sa nako ea 'nete ea PCR
Tlhaloso ea Kit:
- Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 20 feela ho phethela motsoako oa cDNA ea pele.
- Sistimi e phahameng ea sensitivity reverse transcript, litempele tsa boemo ba pg le tsona li ka fumana cDNA ea boleng bo holimo.
- Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso oa karabelo ea sistimi e ka ba holimo ho 50 ℃, 'me e na le ts'ebetso e ntle ea ho fetolela ka morao.
- Esita le disampole tse sa hloekang tsa RNA (joala bo fihlang ho 60%, letsoai la guanidine ho fihla ho 750mM) li ka ba tlas'a karabelo e fapaneng ea transcript.
- 2× RT KAPA-E bonoloTM Mix e kentse li-primers tse ntlafalitsoeng tsa khatiso (Random Primer, Oligo(dT)18 Sethome).
Litlhaloso tsa Kit
Foregene's 2× RT KAPA-E bonoloTMMotsoako o ka kopanya cDNA ea strand ea pele mochesong o ka holimo ho 50 ° C, e leng se loketseng karabelo ea morao-rao ea litempele tse rarahaneng tsa RNA.
RNA e hloekisitsoeng ka laboratoring hangata e na le masala a joala le letsoai la guanidine, tse nang le thepa e matla ea ho thibela ho li-reverse transcriptases, e leng se bakang liphello tse sa thabiseng tsa ho ngola reverse kapa katleho e tlaase ea ho ngola.Foregene 2 × RT Mix e bonts'a mamello e phahameng haholo ea joala le letsoai la guanidine.Mamello e phahameng ka ho fetisisa ea joala ho lisampole tsa RNA ke 60%, 'me mamello e phahameng ka ho fetisisa ea letsoai la guanidine ke 750mM.Esita le RNA e sa hloekang e ka sebelisoa bakeng sa karabelo ea reverse transcript le Foregene 2 × RT OR-EasyTMKopanya.
2× RT KAPA-E bonoloTMMix ke sesebelisoa sa reverse reverse transcript se etselitsoeng nako ea 'nete PCR.Sistimi ena e sebetsa hantle haholo 'me ha e hloke ho kenya li-primers,' me e khona ho etsa li-reverse transcript ho litempele tse nyane tsa RNA.Sistimi e ikhethileng ea karabelo e etsa hore karabelo ea RT e be bonolo, e potlake hape e sebetse hantle.The strand cDNA synthesis ea pele e ka phethoa ka metsotso e 20.Sehlahisoa sena se ka sebelisoa hammoho le sehlahisoa sa Foregene's fluorescent quantitative Real Time PCR Easy.TMho fumana liphetho tsa liteko tsa boleng bo holimo.
Litlhaloso
| RT BonoloTMII Master Premix bakeng sa motsoako oa pele oa cDNA bakeng sa Real Time PCR | |||
| Likarolo tsa Kit | RT-01021 | RT-01022 | RT-01023 |
| 50 T (10μl sistimi) | 200 T(10μl sistimi) | 800 T (10μl sistimi) | |
| 2× RT KAPA-E bonoloTMKopanya | 0.25ml | 1ml | 1ml × 4 |
| RNase-Free ddH2O | 1.7ml | 1.7ml | 1.7ml × 3 |
| Buka ea Tataiso | 1 sengoathoana | 1 sengoathoana | 1 sengoathoana |
Likarolo le melemo
- Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 20 feela ho phethela motsoako oa cDNA ea pele.
- Sistimi e phahameng ea sensitivity reverse transcript, litempele tsa boemo ba pg le tsona li ka fumana cDNA ea boleng bo holimo.
- Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso oa karabelo ea sistimi e ka ba holimo ho 50 ℃, 'me e na le ts'ebetso e ntle ea ho fetolela ka morao.
- Esita le disampole tse sa hloekang tsa RNA (joala bo fihlang ho 60%, letsoai la guanidine ho fihla ho 750mM) li ka ba tlas'a karabelo e fapaneng ea transcript.
- 2× RT KAPA-E bonoloTMMix e kentse li-primers tse ntlafalitsoeng tsa khatiso (Random Primer, Oligo(dT)18Sethome).
Sesebelisoa sa Kit
- E sebelisoa ka kotloloho ho Nako ea 'Nete ea PCR ho sekaseka ka bongata polelo ea lefutso
- E khona ho sekaseka kapele le ka nepo palo e nyane ea RNA joalo ka livaerase tsa RNA
- Phetolelo ea morao ea litempele tsa RNA tse nang le litaba tse phahameng tsa GC kapa sebopeho se rarahaneng sa bobeli
Phallo ea mosebetsi
Polokelo le shelf bophelo
1. Maemo a lipalangoang
Ts'ebetso eohle ea lipalangoang tse tlase tsa mocheso o tlase oa leqhoa, ho netefatsa hore khiti e boemong ba <4°C.
2. Maemo a polokelo
RT BonoloTMII e bolokiloe ho -20°C.Boloka sehlahisoa ka sehatsetsing se tsitsitseng sa mocheso ho -20 ° C hang ka mor'a ho fumana.Haeba maemo a polokelo a loketse, sehlahisoa se ke ke sa nyenyefatsa ts'ebetso leha e le efe nakong ea selemo sa 1 sa ho netefatsa.
