China Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish) Moetsi le Mofani |Foregene

Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)

Tlhaloso ea Kit:

Tšebeliso e tobileng ea li-zebrafish lysate e le template bakeng sa ho hōlisa PCR, ha ho na ho ntšoa ka thōko ho DNA, ka potlako le ka kutloisiso e phahameng.E kenyellelitse UNG tsamaiso e khahlanong le tšilafalo ho felisa lintho tse fosahetseng.

◮Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.

◮Tlhokahalo ea sampole e nyane, nka peo e le 'ngoe feela.

◮Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, 'me ho buuoa ho bonolo.

◮Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Litlhaloso

Setsi sena se sebelisa mokhoa o ikhethileng oa lysis buffer system ho lokolla kapele DNA ea genomic ho liqoaha le lithane tse ling tsa tlhapi ea metsi a hloekileng, mapheoana a mohatla kapa lisampole tsa mahe a tlhapi bakeng sa karabelo ea PCR, ka hona e loketse haholo tlhahlobo e kholo ea liphatsa tsa lefutso.Mokhoa oa ho lokolla DNA ea genomic ho tswa ho lysis buffer e phethoa ka hare ho metsotso e 10-30 ho 65 ° C.Ha ho na mekhoa e meng e kang ho tlosoa ha protheine le RNA e hlokahalang, 'me DNA ea trace e lokolotsoeng e ka sebelisoa e le template bakeng sa karabelo ea PCR.

2 × PCR EasyTM Mix (UNG) e sebelisa dUTP ho e-na le dTTP motheong oa 2 × PCR EasyTM Mix, mme e eketsa enzyme ea UNG (Uracil-N-glycosylase) e ka senyang template e nang le dUTP ka nako e le 'ngoe.Pele ho karabelo ea PCR, enzyme ea UNG e sebelisoa ho nyenyefatsa sehlahisoa sa PCR se nang le uracil.Enzyme ea UNG e ke ke ea e-ba le phello leha e le efe ho template e se nang uracil, ka tsela eo e netefatsa ho nepahala le ho nepahala ha amplification le ho thibela monyetla oa tlhahlobo e kholo ea liphatsa tsa lefutso.Tšoaetso ea lihlahisoa tsa PCR e etsahetse.

D-Taq DNA polymerase ke DNA polymerase e entsoeng ka ho khetheha ke Foregene bakeng sa karabelo e tobileng ea PCR.D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho holisa DNA ka mokhoa o nepahetseng lits'ebetsong tse fapaneng tse rarahaneng tsa karabelo, mme lebelo la amplification le ka fihla ho 2Kb/min, e loketseng ka ho khetheha bakeng sa karabelo ea Direct PCR.

Litlhaloso

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Likarolo tsa Kit

Karolo ea I	Buffer FP
	Foregene Protease
	6× DNA Loading Buffer
Karolo ea II	2 × PCR e bonolo^TMMotsoako(UNG)
Litaelo

Likarolo le melemo

■ Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.

■ Tlhokahalo ea sampole e nyane, joalo ka ha 1mg tlhapi ea caudal fin kapa mahe a 10 a tlhapi a ka lekoa.

■ Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, ’me ho buuoa ho bonolo.

■ Sistimi ea PCR e khahlanong le tšilafalo ea 2× PCR EasyTM Mix (UNG), e ka felisang ka katleho tšilafalo e bakoang ke lihlahisoa tsa PCR le ho netefatsa ho nepahala le ho nepahala ha amplification.

■ Sistimi e ntlafalitsoeng ea PCR e etsa hore PCR e be le maemo a holimo le mamello e matla ho PCR reaction inhibitors.

Litekanyetso tsa kit

Kopo: Litlhapi tsa Zebra le litlhapi tse ling tsa metsi a hloekileng.

DNA e lokollotsoeng ho tsoa ho sampole lysis: e sebelisoa feela joalo ka template ea PCR.

Thepa e ka sebelisoa bakeng sa merero e latelang: ho tsebahatsa transgenosis, genotyping ea liphoofolo, joalo-joalo.

Mokhoa oa ho sebetsa

Storage le Shelf bophelo

Karolo ea I ea kit ena e lokela ho bolokoa ho 2-8 ℃.

Reagent Buffer FP e ka bolokoa likhoeli tse 12 tlas'a maemo a omileng;e ka bolokoa ho 2-8 ℃ bakeng sa polokelo e telele.

v Reagent Foregene Protease e na le foromo e ikhethang.Ho netefatsa ts'ebetso ea eona le botsitso, ka kopo e boloke ho 4 ℃ bakeng sa likhoeli tse 12.

vReagent 6 × DNA Loading Buffer e ka bolokoa ho 4 ℃ kapa -20 ℃ nako e telele.

Karolo ea II ea kit ena e lokela ho bolokoa ho -20 ℃.

vReagent 2 × PCR EasyTM Mix(UNG), haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4 ℃ bakeng sa polokelo ea nako e khutšoanyane (sebelisa pele ho matsatsi a 10).

E fetileng: Zebra Fish Direct PCR Kit Direct PCR Lysis Reagent(zebrafish)

E 'ngoe: Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Mohatla)(bakeng sa Genotyping)

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